Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1542 and GFF1543 are separated by 7 nucleotides GFF1543 and GFF1544 are separated by 40 nucleotides GFF1544 and GFF1545 are separated by 104 nucleotides GFF1545 and GFF1546 are separated by 36 nucleotides
GFF1542 - hypothetical protein, at 190,133 to 190,453
GFF1542
GFF1543 - 3-oxoacyl-[acyl-carrier-protein] reductase FabG, at 190,461 to 191,213
GFF1543
GFF1544 - hypothetical protein, at 191,254 to 192,423
GFF1544
GFF1545 - tRNA (guanine-N(7)-)-methyltransferase, at 192,528 to 193,307
GFF1545
GFF1546 - putative GTP-binding protein EngB, at 193,344 to 193,979
GFF1546
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1 at 190.270 kb on - strand, within GFF1542 at 190.382 kb on - strand, within GFF1542 at 190.444 kb on + strand at 190.445 kb on - strand at 190.457 kb on + strand at 190.462 kb on + strand at 190.462 kb on + strand at 190.462 kb on + strand at 190.462 kb on + strand at 190.463 kb on - strand at 190.463 kb on - strand at 190.463 kb on - strand at 190.463 kb on - strand at 190.467 kb on - strand at 190.467 kb on - strand at 190.470 kb on + strand at 190.470 kb on + strand at 190.470 kb on + strand at 190.471 kb on - strand at 190.471 kb on - strand at 190.471 kb on - strand at 190.471 kb on - strand at 190.471 kb on - strand at 190.471 kb on - strand at 190.525 kb on + strand at 190.526 kb on - strand at 190.526 kb on - strand at 190.677 kb on + strand, within GFF1543 at 190.677 kb on + strand, within GFF1543 at 190.678 kb on - strand, within GFF1543 at 190.744 kb on + strand, within GFF1543 at 190.744 kb on + strand, within GFF1543 at 190.744 kb on + strand, within GFF1543 at 190.745 kb on - strand, within GFF1543 at 190.745 kb on - strand, within GFF1543 at 190.745 kb on - strand, within GFF1543 at 190.745 kb on - strand, within GFF1543 at 190.745 kb on - strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.753 kb on + strand, within GFF1543 at 190.754 kb on - strand, within GFF1543 at 190.754 kb on - strand, within GFF1543 at 190.754 kb on - strand, within GFF1543 at 190.754 kb on - strand, within GFF1543 at 190.773 kb on + strand, within GFF1543 at 190.774 kb on - strand, within GFF1543 at 190.774 kb on - strand, within GFF1543 at 190.774 kb on - strand, within GFF1543 at 190.774 kb on - strand, within GFF1543 at 190.859 kb on + strand, within GFF1543 at 190.859 kb on + strand, within GFF1543 at 190.860 kb on - strand, within GFF1543 at 190.860 kb on - strand, within GFF1543 at 190.860 kb on - strand, within GFF1543 at 190.921 kb on + strand, within GFF1543 at 190.922 kb on - strand, within GFF1543 at 190.922 kb on - strand, within GFF1543 at 190.922 kb on - strand, within GFF1543 at 190.924 kb on + strand, within GFF1543 at 190.924 kb on + strand, within GFF1543 at 190.925 kb on - strand, within GFF1543 at 191.034 kb on + strand, within GFF1543 at 191.034 kb on + strand, within GFF1543 at 191.035 kb on - strand, within GFF1543 at 191.035 kb on - strand, within GFF1543 at 191.035 kb on - strand, within GFF1543 at 191.178 kb on - strand at 191.184 kb on + strand at 191.192 kb on + strand at 191.192 kb on + strand at 191.192 kb on + strand at 191.192 kb on + strand at 191.198 kb on + strand at 191.199 kb on - strand at 191.218 kb on + strand at 191.218 kb on + strand at 191.218 kb on + strand at 191.219 kb on - strand at 191.219 kb on - strand at 191.219 kb on - strand at 191.219 kb on - strand at 191.219 kb on - strand at 191.229 kb on - strand at 191.242 kb on + strand at 191.242 kb on + strand at 191.242 kb on + strand at 191.242 kb on + strand at 191.242 kb on + strand at 191.242 kb on + strand at 191.243 kb on - strand at 191.243 kb on - strand at 191.243 kb on - strand at 191.243 kb on - strand at 191.243 kb on - strand at 191.244 kb on + strand at 191.244 kb on + strand at 191.245 kb on - strand at 191.245 kb on - strand at 191.245 kb on - strand at 191.245 kb on - strand at 191.401 kb on + strand, within GFF1544 at 191.440 kb on - strand, within GFF1544 at 191.451 kb on - strand, within GFF1544 at 191.558 kb on + strand, within GFF1544 at 191.990 kb on + strand, within GFF1544 at 192.044 kb on - strand, within GFF1544 at 192.450 kb on + strand at 192.450 kb on + strand at 192.451 kb on - strand at 192.451 kb on - strand at 192.451 kb on - strand at 192.451 kb on - strand at 192.451 kb on - strand at 192.451 kb on - strand at 192.510 kb on + strand at 192.510 kb on + strand at 192.584 kb on + strand at 192.584 kb on + strand at 192.584 kb on + strand at 192.584 kb on + strand at 192.584 kb on + strand at 192.584 kb on + strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.585 kb on - strand at 192.586 kb on + strand at 192.586 kb on + strand at 192.586 kb on + strand at 192.607 kb on + strand, within GFF1545 at 192.640 kb on - strand, within GFF1545 at 192.706 kb on + strand, within GFF1545 at 192.738 kb on + strand, within GFF1545 at 192.738 kb on + strand, within GFF1545 at 192.738 kb on + strand, within GFF1545 at 192.738 kb on + strand, within GFF1545 at 192.859 kb on - strand, within GFF1545 at 192.938 kb on + strand, within GFF1545 at 192.944 kb on + strand, within GFF1545 at 192.944 kb on + strand, within GFF1545 at 192.944 kb on + strand, within GFF1545 at 192.945 kb on - strand, within GFF1545 at 192.947 kb on + strand, within GFF1545 at 192.947 kb on + strand, within GFF1545 at 192.948 kb on - strand, within GFF1545 at 192.973 kb on + strand, within GFF1545 at 192.974 kb on - strand, within GFF1545 at 193.010 kb on - strand, within GFF1545 at 193.010 kb on - strand, within GFF1545 at 193.147 kb on - strand, within GFF1545 at 193.159 kb on + strand, within GFF1545 at 193.168 kb on + strand, within GFF1545 at 193.169 kb on - strand, within GFF1545 at 193.197 kb on - strand, within GFF1545 at 193.210 kb on - strand, within GFF1545 at 193.227 kb on + strand, within GFF1545 at 193.227 kb on + strand, within GFF1545 at 193.305 kb on + strand at 193.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 190,270 - GFF1542 0.43 -1.3 190,382 - GFF1542 0.78 -1.3 190,444 + +0.0 190,445 - -2.0 190,457 + -1.0 190,462 + +0.8 190,462 + -0.2 190,462 + +0.3 190,462 + +1.2 190,463 - -0.2 190,463 - +0.6 190,463 - +0.0 190,463 - +0.4 190,467 - -0.7 190,467 - -0.2 190,470 + -0.1 190,470 + +0.0 190,470 + -1.6 190,471 - -1.6 190,471 - -0.4 190,471 - +1.0 190,471 - +0.7 190,471 - -1.1 190,471 - -0.4 190,525 + -2.0 190,526 - -1.2 190,526 - +0.0 190,677 + GFF1543 0.29 +0.2 190,677 + GFF1543 0.29 +0.7 190,678 - GFF1543 0.29 -1.1 190,744 + GFF1543 0.38 -1.0 190,744 + GFF1543 0.38 +0.0 190,744 + GFF1543 0.38 -0.1 190,745 - GFF1543 0.38 +0.0 190,745 - GFF1543 0.38 -0.8 190,745 - GFF1543 0.38 -0.8 190,745 - GFF1543 0.38 -0.3 190,745 - GFF1543 0.38 -1.1 190,753 + GFF1543 0.39 -1.3 190,753 + GFF1543 0.39 +0.4 190,753 + GFF1543 0.39 +0.7 190,753 + GFF1543 0.39 +0.6 190,753 + GFF1543 0.39 +0.5 190,753 + GFF1543 0.39 -0.7 190,753 + GFF1543 0.39 -0.6 190,754 - GFF1543 0.39 -1.0 190,754 - GFF1543 0.39 +0.0 190,754 - GFF1543 0.39 -1.9 190,754 - GFF1543 0.39 -0.6 190,773 + GFF1543 0.41 -1.0 190,774 - GFF1543 0.42 -2.3 190,774 - GFF1543 0.42 +0.3 190,774 - GFF1543 0.42 -0.6 190,774 - GFF1543 0.42 -1.2 190,859 + GFF1543 0.53 -0.4 190,859 + GFF1543 0.53 +0.0 190,860 - GFF1543 0.53 +0.4 190,860 - GFF1543 0.53 -0.9 190,860 - GFF1543 0.53 -1.4 190,921 + GFF1543 0.61 -0.3 190,922 - GFF1543 0.61 -1.2 190,922 - GFF1543 0.61 -0.1 190,922 - GFF1543 0.61 -0.3 190,924 + GFF1543 0.61 +0.2 190,924 + GFF1543 0.61 -0.9 190,925 - GFF1543 0.62 -0.3 191,034 + GFF1543 0.76 -0.6 191,034 + GFF1543 0.76 -0.6 191,035 - GFF1543 0.76 -1.0 191,035 - GFF1543 0.76 +0.7 191,035 - GFF1543 0.76 -1.6 191,178 - -0.2 191,184 + -1.6 191,192 + -0.6 191,192 + -1.3 191,192 + -1.8 191,192 + -0.6 191,198 + -1.0 191,199 - -1.2 191,218 + -0.6 191,218 + +0.2 191,218 + +0.4 191,219 - -2.0 191,219 - +0.4 191,219 - -0.9 191,219 - +0.1 191,219 - -1.3 191,229 - -1.4 191,242 + +1.4 191,242 + +1.0 191,242 + +0.3 191,242 + -0.5 191,242 + -0.2 191,242 + -1.6 191,243 - +0.6 191,243 - -1.0 191,243 - -1.5 191,243 - +0.1 191,243 - -1.6 191,244 + -2.0 191,244 + -1.6 191,245 - -0.6 191,245 - -0.4 191,245 - +0.1 191,245 - -1.0 191,401 + GFF1544 0.13 -1.0 191,440 - GFF1544 0.16 +0.0 191,451 - GFF1544 0.17 -1.0 191,558 + GFF1544 0.26 +0.0 191,990 + GFF1544 0.63 +1.0 192,044 - GFF1544 0.68 -1.0 192,450 + -1.3 192,450 + -1.3 192,451 - +0.1 192,451 - -0.9 192,451 - -0.5 192,451 - -0.4 192,451 - -1.0 192,451 - +0.6 192,510 + -1.7 192,510 + +0.2 192,584 + -0.9 192,584 + +0.7 192,584 + -2.0 192,584 + +0.5 192,584 + -0.9 192,584 + +1.0 192,585 - -0.2 192,585 - -0.3 192,585 - -0.4 192,585 - +0.1 192,585 - +0.2 192,585 - -1.0 192,585 - +0.0 192,585 - -0.1 192,586 + +0.3 192,586 + +0.2 192,586 + -1.0 192,607 + GFF1545 0.10 -0.3 192,640 - GFF1545 0.14 +1.0 192,706 + GFF1545 0.23 -0.6 192,738 + GFF1545 0.27 -1.8 192,738 + GFF1545 0.27 +0.0 192,738 + GFF1545 0.27 -0.2 192,738 + GFF1545 0.27 -1.0 192,859 - GFF1545 0.42 -1.2 192,938 + GFF1545 0.53 +1.0 192,944 + GFF1545 0.53 +0.0 192,944 + GFF1545 0.53 +0.4 192,944 + GFF1545 0.53 -1.3 192,945 - GFF1545 0.53 -1.0 192,947 + GFF1545 0.54 +1.3 192,947 + GFF1545 0.54 -1.0 192,948 - GFF1545 0.54 -0.9 192,973 + GFF1545 0.57 +0.2 192,974 - GFF1545 0.57 +0.3 193,010 - GFF1545 0.62 +0.2 193,010 - GFF1545 0.62 -1.3 193,147 - GFF1545 0.79 -1.6 193,159 + GFF1545 0.81 -0.7 193,168 + GFF1545 0.82 +0.0 193,169 - GFF1545 0.82 -1.0 193,197 - GFF1545 0.86 +1.2 193,210 - GFF1545 0.87 -0.7 193,227 + GFF1545 0.90 -0.3 193,227 + GFF1545 0.90 +0.0 193,305 + -0.8 193,346 - +1.0
Or see this region's nucleotide sequence