Experiment: Trimethoprim stress 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS17695 and ABZR88_RS17700 are separated by 12 nucleotides ABZR88_RS17700 and ABZR88_RS17705 are separated by 112 nucleotides ABZR88_RS17705 and ABZR88_RS17710 are separated by 18 nucleotides
ABZR88_RS17695: ABZR88_RS17695 - polysaccharide ABC transporter ATP-binding protein, at 4,325,213 to 4,326,535
_RS17695
ABZR88_RS17700: ABZR88_RS17700 - ABC transporter permease, at 4,326,548 to 4,327,402
_RS17700
ABZR88_RS17705: ABZR88_RS17705 - tyrosine-protein kinase, at 4,327,515 to 4,329,938
_RS17705
ABZR88_RS17710: ABZR88_RS17710 - polysaccharide biosynthesis/export family protein, at 4,329,957 to 4,330,736
_RS17710
Position (kb)
4327
4328
4329
4330 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4326.631 kb on + strand at 4326.719 kb on + strand, within ABZR88_RS17700 at 4326.777 kb on + strand, within ABZR88_RS17700 at 4326.911 kb on - strand, within ABZR88_RS17700 at 4326.911 kb on - strand, within ABZR88_RS17700 at 4326.912 kb on + strand, within ABZR88_RS17700 at 4326.912 kb on + strand, within ABZR88_RS17700 at 4326.992 kb on + strand, within ABZR88_RS17700 at 4327.121 kb on + strand, within ABZR88_RS17700 at 4327.210 kb on + strand, within ABZR88_RS17700 at 4327.452 kb on - strand at 4327.452 kb on - strand at 4327.489 kb on + strand at 4327.676 kb on + strand at 4327.676 kb on + strand at 4327.735 kb on - strand at 4327.739 kb on + strand at 4327.739 kb on + strand at 4327.762 kb on + strand, within ABZR88_RS17705 at 4327.766 kb on + strand, within ABZR88_RS17705 at 4327.810 kb on - strand, within ABZR88_RS17705 at 4327.897 kb on - strand, within ABZR88_RS17705 at 4327.985 kb on + strand, within ABZR88_RS17705 at 4328.031 kb on - strand, within ABZR88_RS17705 at 4328.115 kb on - strand, within ABZR88_RS17705 at 4328.120 kb on + strand, within ABZR88_RS17705 at 4328.177 kb on + strand, within ABZR88_RS17705 at 4328.222 kb on + strand, within ABZR88_RS17705 at 4328.502 kb on - strand, within ABZR88_RS17705 at 4328.511 kb on - strand, within ABZR88_RS17705 at 4328.567 kb on - strand, within ABZR88_RS17705 at 4328.570 kb on + strand, within ABZR88_RS17705 at 4328.577 kb on - strand, within ABZR88_RS17705 at 4328.814 kb on + strand, within ABZR88_RS17705 at 4328.924 kb on - strand, within ABZR88_RS17705 at 4329.189 kb on - strand, within ABZR88_RS17705 at 4329.446 kb on - strand, within ABZR88_RS17705 at 4329.551 kb on - strand, within ABZR88_RS17705 at 4329.597 kb on + strand, within ABZR88_RS17705 at 4329.620 kb on + strand, within ABZR88_RS17705 at 4329.660 kb on - strand, within ABZR88_RS17705 at 4329.810 kb on + strand at 4329.851 kb on - strand at 4329.909 kb on + strand at 4330.013 kb on + strand at 4330.014 kb on - strand at 4330.140 kb on + strand, within ABZR88_RS17710 at 4330.333 kb on - strand, within ABZR88_RS17710 at 4330.440 kb on - strand, within ABZR88_RS17710 at 4330.529 kb on + strand, within ABZR88_RS17710 at 4330.662 kb on - strand at 4330.664 kb on + strand at 4330.665 kb on - strand at 4330.816 kb on - strand at 4330.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim stress 0.05 mg/ml remove 4,326,631 + -1.6 4,326,719 + ABZR88_RS17700 0.20 -1.2 4,326,777 + ABZR88_RS17700 0.27 -1.9 4,326,911 - ABZR88_RS17700 0.42 -1.4 4,326,911 - ABZR88_RS17700 0.42 -0.3 4,326,912 + ABZR88_RS17700 0.43 -0.8 4,326,912 + ABZR88_RS17700 0.43 -1.5 4,326,992 + ABZR88_RS17700 0.52 -1.2 4,327,121 + ABZR88_RS17700 0.67 -1.4 4,327,210 + ABZR88_RS17700 0.77 -1.7 4,327,452 - -0.1 4,327,452 - -0.1 4,327,489 + -0.7 4,327,676 + -1.4 4,327,676 + -1.5 4,327,735 - -1.3 4,327,739 + -1.4 4,327,739 + -1.9 4,327,762 + ABZR88_RS17705 0.10 -2.0 4,327,766 + ABZR88_RS17705 0.10 -1.6 4,327,810 - ABZR88_RS17705 0.12 -3.3 4,327,897 - ABZR88_RS17705 0.16 -1.8 4,327,985 + ABZR88_RS17705 0.19 -1.1 4,328,031 - ABZR88_RS17705 0.21 -0.7 4,328,115 - ABZR88_RS17705 0.25 -2.3 4,328,120 + ABZR88_RS17705 0.25 -1.0 4,328,177 + ABZR88_RS17705 0.27 -1.7 4,328,222 + ABZR88_RS17705 0.29 -0.4 4,328,502 - ABZR88_RS17705 0.41 +1.6 4,328,511 - ABZR88_RS17705 0.41 -1.0 4,328,567 - ABZR88_RS17705 0.43 -2.9 4,328,570 + ABZR88_RS17705 0.44 -1.1 4,328,577 - ABZR88_RS17705 0.44 -0.2 4,328,814 + ABZR88_RS17705 0.54 -0.9 4,328,924 - ABZR88_RS17705 0.58 -0.6 4,329,189 - ABZR88_RS17705 0.69 -2.3 4,329,446 - ABZR88_RS17705 0.80 -0.5 4,329,551 - ABZR88_RS17705 0.84 +0.2 4,329,597 + ABZR88_RS17705 0.86 -1.2 4,329,620 + ABZR88_RS17705 0.87 -1.8 4,329,660 - ABZR88_RS17705 0.88 -1.5 4,329,810 + -0.7 4,329,851 - -0.7 4,329,909 + -0.0 4,330,013 + -0.9 4,330,014 - -0.9 4,330,140 + ABZR88_RS17710 0.23 -0.9 4,330,333 - ABZR88_RS17710 0.48 -0.4 4,330,440 - ABZR88_RS17710 0.62 -0.6 4,330,529 + ABZR88_RS17710 0.73 +0.9 4,330,662 - -0.1 4,330,664 + -0.6 4,330,665 - -2.2 4,330,816 - -0.3 4,330,930 + +0.4
Or see this region's nucleotide sequence