Experiment: Kanamycin sulfate stress 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS14070 and ABZR88_RS14075 are separated by 118 nucleotides ABZR88_RS14075 and ABZR88_RS14080 are separated by 5 nucleotides ABZR88_RS14080 and ABZR88_RS14085 are separated by 198 nucleotides ABZR88_RS14085 and ABZR88_RS14090 are separated by 21 nucleotides ABZR88_RS14090 and ABZR88_RS14095 overlap by 1 nucleotides
ABZR88_RS14070: ABZR88_RS14070 - phosphoglucosamine mutase, at 3,498,569 to 3,499,957
_RS14070
ABZR88_RS14075: ABZR88_RS14075 - phosphatase PAP2 family protein, at 3,500,076 to 3,500,639
_RS14075
ABZR88_RS14080: ABZR88_RS14080 - ZIP family metal transporter, at 3,500,645 to 3,501,367
_RS14080
ABZR88_RS14085: ABZR88_RS14085 - MarR family winged helix-turn-helix transcriptional regulator, at 3,501,566 to 3,502,018
_RS14085
ABZR88_RS14090: ABZR88_RS14090 - DUF5522 domain-containing protein, at 3,502,040 to 3,502,219
_RS14090
ABZR88_RS14095: ABZR88_RS14095 - MBL fold metallo-hydrolase, at 3,502,219 to 3,503,157
_RS14095
Position (kb)
3500
3501
3502 Strain fitness (log2 ratio)
-1
0
1 at 3499.963 kb on - strand at 3499.969 kb on - strand at 3499.996 kb on - strand at 3499.997 kb on + strand at 3499.998 kb on - strand at 3500.052 kb on + strand at 3501.025 kb on - strand, within ABZR88_RS14080 at 3501.460 kb on + strand at 3501.461 kb on - strand at 3501.522 kb on - strand at 3501.580 kb on + strand at 3501.707 kb on + strand, within ABZR88_RS14085 at 3501.771 kb on - strand, within ABZR88_RS14085 at 3501.969 kb on + strand, within ABZR88_RS14085 at 3502.193 kb on - strand, within ABZR88_RS14090 at 3502.198 kb on - strand, within ABZR88_RS14090 at 3502.204 kb on + strand at 3502.268 kb on + strand at 3502.318 kb on + strand, within ABZR88_RS14095 at 3502.333 kb on - strand, within ABZR88_RS14095
Per-strain Table
Position Strand Gene LocusTag Fraction Kanamycin sulfate stress 0.05 mg/ml remove 3,499,963 - -0.7 3,499,969 - +0.0 3,499,996 - -0.2 3,499,997 + +0.0 3,499,998 - -0.5 3,500,052 + -0.7 3,501,025 - ABZR88_RS14080 0.53 -0.8 3,501,460 + -0.3 3,501,461 - +0.1 3,501,522 - -0.0 3,501,580 + -1.0 3,501,707 + ABZR88_RS14085 0.31 -0.8 3,501,771 - ABZR88_RS14085 0.45 -1.2 3,501,969 + ABZR88_RS14085 0.89 -1.0 3,502,193 - ABZR88_RS14090 0.85 -0.1 3,502,198 - ABZR88_RS14090 0.88 -0.4 3,502,204 + -0.3 3,502,268 + +0.1 3,502,318 + ABZR88_RS14095 0.11 +0.0 3,502,333 - ABZR88_RS14095 0.12 -0.9
Or see this region's nucleotide sequence