Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4021 and GFF4022 are separated by 32 nucleotides GFF4022 and GFF4023 are separated by 39 nucleotides GFF4023 and GFF4024 overlap by 4 nucleotides
GFF4021 - 2-keto-4-carboxy-3-hexenedioate hydratase, at 4,761 to 5,744
GFF4021
GFF4022 - Betaine aldehyde dehydrogenase, at 5,777 to 7,318
GFF4022
GFF4023 - High-affinity branched-chain amino acid transport system permease protein LivH, at 7,358 to 8,209
GFF4023
GFF4024 - hypothetical protein, at 8,206 to 9,195
GFF4024
Position (kb) 5
6
7
8 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.802 kb on + strand at 4.803 kb on - strand at 5.001 kb on + strand, within GFF4021 at 5.002 kb on - strand, within GFF4021 at 5.283 kb on + strand, within GFF4021 at 5.283 kb on + strand, within GFF4021 at 5.316 kb on + strand, within GFF4021 at 5.317 kb on - strand, within GFF4021 at 5.319 kb on + strand, within GFF4021 at 5.449 kb on - strand, within GFF4021 at 5.449 kb on - strand, within GFF4021 at 5.539 kb on - strand, within GFF4021 at 5.577 kb on + strand, within GFF4021 at 5.594 kb on - strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.608 kb on + strand, within GFF4021 at 5.609 kb on - strand, within GFF4021 at 5.609 kb on - strand, within GFF4021 at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.665 kb on + strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.666 kb on - strand at 5.753 kb on + strand at 6.039 kb on - strand, within GFF4022 at 6.159 kb on - strand, within GFF4022 at 6.159 kb on - strand, within GFF4022 at 6.159 kb on - strand, within GFF4022 at 6.215 kb on + strand, within GFF4022 at 6.224 kb on + strand, within GFF4022 at 6.224 kb on + strand, within GFF4022 at 6.224 kb on + strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.225 kb on - strand, within GFF4022 at 6.353 kb on + strand, within GFF4022 at 6.629 kb on + strand, within GFF4022 at 6.630 kb on - strand, within GFF4022 at 6.630 kb on - strand, within GFF4022 at 6.630 kb on - strand, within GFF4022 at 6.707 kb on + strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.708 kb on - strand, within GFF4022 at 6.914 kb on + strand, within GFF4022 at 6.914 kb on + strand, within GFF4022 at 6.914 kb on + strand, within GFF4022 at 6.915 kb on - strand, within GFF4022 at 6.915 kb on - strand, within GFF4022 at 7.049 kb on + strand, within GFF4022 at 7.230 kb on - strand at 7.230 kb on - strand at 7.496 kb on + strand, within GFF4023 at 7.496 kb on + strand, within GFF4023 at 8.132 kb on + strand at 8.132 kb on + strand at 8.133 kb on - strand at 8.133 kb on - strand at 8.287 kb on + strand at 8.287 kb on + strand at 8.287 kb on + strand at 8.287 kb on + strand at 8.287 kb on + strand at 8.288 kb on - strand at 8.288 kb on - strand at 8.317 kb on + strand, within GFF4024 at 8.317 kb on + strand, within GFF4024
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 4,802 + -0.2 4,803 - +1.3 5,001 + GFF4021 0.24 -0.1 5,002 - GFF4021 0.24 -1.2 5,283 + GFF4021 0.53 +0.6 5,283 + GFF4021 0.53 -1.1 5,316 + GFF4021 0.56 +0.2 5,317 - GFF4021 0.57 +0.2 5,319 + GFF4021 0.57 -0.5 5,449 - GFF4021 0.70 -0.8 5,449 - GFF4021 0.70 -0.2 5,539 - GFF4021 0.79 -0.8 5,577 + GFF4021 0.83 +0.4 5,594 - GFF4021 0.85 -0.4 5,608 + GFF4021 0.86 +0.6 5,608 + GFF4021 0.86 +1.0 5,608 + GFF4021 0.86 +0.5 5,608 + GFF4021 0.86 -1.0 5,608 + GFF4021 0.86 +0.5 5,609 - GFF4021 0.86 +0.3 5,609 - GFF4021 0.86 +0.4 5,665 + -0.4 5,665 + +2.6 5,665 + -0.3 5,665 + -1.0 5,665 + -0.2 5,665 + +1.1 5,665 + +0.3 5,666 - +0.6 5,666 - +0.2 5,666 - -2.3 5,666 - +0.0 5,666 - -1.7 5,666 - -0.9 5,666 - -0.4 5,666 - -0.3 5,753 + -2.4 6,039 - GFF4022 0.17 -1.5 6,159 - GFF4022 0.25 -0.5 6,159 - GFF4022 0.25 +0.7 6,159 - GFF4022 0.25 -0.7 6,215 + GFF4022 0.28 -0.1 6,224 + GFF4022 0.29 +0.2 6,224 + GFF4022 0.29 +1.4 6,224 + GFF4022 0.29 -1.0 6,225 - GFF4022 0.29 +1.0 6,225 - GFF4022 0.29 +0.9 6,225 - GFF4022 0.29 -0.1 6,225 - GFF4022 0.29 -0.2 6,353 + GFF4022 0.37 -0.1 6,629 + GFF4022 0.55 -0.6 6,630 - GFF4022 0.55 -0.2 6,630 - GFF4022 0.55 +0.3 6,630 - GFF4022 0.55 -0.7 6,707 + GFF4022 0.60 -0.8 6,708 - GFF4022 0.60 +0.0 6,708 - GFF4022 0.60 -1.7 6,708 - GFF4022 0.60 -0.1 6,708 - GFF4022 0.60 -0.2 6,914 + GFF4022 0.74 -0.4 6,914 + GFF4022 0.74 -0.2 6,914 + GFF4022 0.74 -0.8 6,915 - GFF4022 0.74 +0.7 6,915 - GFF4022 0.74 +0.3 7,049 + GFF4022 0.82 +0.1 7,230 - +0.4 7,230 - +0.0 7,496 + GFF4023 0.16 -0.3 7,496 + GFF4023 0.16 -0.3 8,132 + +0.1 8,132 + +0.8 8,133 - +0.3 8,133 - -1.0 8,287 + -0.9 8,287 + -1.7 8,287 + -0.1 8,287 + +0.8 8,287 + -0.6 8,288 - -0.4 8,288 - +0.3 8,317 + GFF4024 0.11 -0.6 8,317 + GFF4024 0.11 +0.7
Or see this region's nucleotide sequence