Experiment: MHII_biocarbonate_serum_Chelated; 35C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS16050 and RR41_RS16055 are separated by 45 nucleotides RR41_RS16055 and RR41_RS16060 overlap by 4 nucleotides RR41_RS16060 and RR41_RS16065 are separated by 124 nucleotides RR41_RS16065 and RR41_RS16070 overlap by 32 nucleotides
RR41_RS16050: RR41_RS16050 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,331,641 to 3,332,450
_RS16050
RR41_RS16055: RR41_RS16055 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,332,496 to 3,332,978
_RS16055
RR41_RS16060: RR41_RS16060 - polynucleotide adenylyltransferase PcnB, at 3,332,975 to 3,334,438
_RS16060
RR41_RS16065: RR41_RS16065 - ComEA family DNA-binding protein, at 3,334,563 to 3,334,973
_RS16065
RR41_RS16070: RR41_RS16070 - nucleoside triphosphate pyrophosphohydrolase, at 3,334,942 to 3,335,748
_RS16070
Position (kb)
3332
3333
3334
3335 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3331.994 kb on + strand, within RR41_RS16050 at 3332.052 kb on + strand, within RR41_RS16050 at 3332.439 kb on + strand at 3332.482 kb on + strand at 3332.482 kb on + strand at 3332.483 kb on - strand at 3332.485 kb on + strand at 3332.486 kb on - strand at 3332.497 kb on + strand at 3332.521 kb on - strand at 3333.098 kb on - strand at 3333.098 kb on - strand at 3333.244 kb on + strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.247 kb on - strand, within RR41_RS16060 at 3333.247 kb on - strand, within RR41_RS16060 at 3333.350 kb on + strand, within RR41_RS16060 at 3333.350 kb on + strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.353 kb on + strand, within RR41_RS16060 at 3333.354 kb on - strand, within RR41_RS16060 at 3333.354 kb on - strand, within RR41_RS16060 at 3333.354 kb on - strand, within RR41_RS16060 at 3333.458 kb on - strand, within RR41_RS16060 at 3333.481 kb on + strand, within RR41_RS16060 at 3333.481 kb on + strand, within RR41_RS16060 at 3333.482 kb on - strand, within RR41_RS16060 at 3333.482 kb on - strand, within RR41_RS16060 at 3333.482 kb on - strand, within RR41_RS16060 at 3333.560 kb on + strand, within RR41_RS16060 at 3333.609 kb on + strand, within RR41_RS16060 at 3333.610 kb on - strand, within RR41_RS16060 at 3333.659 kb on - strand, within RR41_RS16060 at 3333.679 kb on + strand, within RR41_RS16060 at 3333.749 kb on + strand, within RR41_RS16060 at 3333.750 kb on - strand, within RR41_RS16060 at 3333.752 kb on + strand, within RR41_RS16060 at 3333.752 kb on + strand, within RR41_RS16060 at 3333.753 kb on - strand, within RR41_RS16060 at 3333.753 kb on - strand, within RR41_RS16060 at 3333.799 kb on - strand, within RR41_RS16060 at 3333.799 kb on - strand, within RR41_RS16060 at 3333.799 kb on - strand, within RR41_RS16060 at 3333.799 kb on - strand, within RR41_RS16060 at 3333.799 kb on - strand, within RR41_RS16060 at 3333.799 kb on - strand, within RR41_RS16060 at 3333.827 kb on + strand, within RR41_RS16060 at 3333.890 kb on - strand, within RR41_RS16060 at 3333.890 kb on - strand, within RR41_RS16060 at 3333.903 kb on - strand, within RR41_RS16060 at 3334.016 kb on - strand, within RR41_RS16060 at 3334.016 kb on - strand, within RR41_RS16060 at 3334.033 kb on - strand, within RR41_RS16060 at 3334.036 kb on - strand, within RR41_RS16060 at 3334.036 kb on - strand, within RR41_RS16060 at 3334.043 kb on - strand, within RR41_RS16060 at 3334.186 kb on - strand, within RR41_RS16060 at 3334.301 kb on + strand at 3334.302 kb on - strand at 3334.307 kb on + strand at 3334.308 kb on - strand at 3334.334 kb on - strand at 3334.334 kb on - strand at 3334.334 kb on - strand at 3334.334 kb on - strand at 3334.336 kb on - strand at 3334.336 kb on - strand at 3334.454 kb on - strand at 3334.454 kb on - strand at 3334.466 kb on - strand at 3334.466 kb on - strand at 3334.469 kb on + strand at 3334.470 kb on - strand at 3334.489 kb on + strand at 3334.489 kb on + strand at 3334.489 kb on + strand at 3334.489 kb on + strand at 3334.490 kb on - strand at 3334.490 kb on - strand at 3334.490 kb on - strand at 3334.490 kb on - strand at 3334.495 kb on + strand at 3334.496 kb on - strand at 3334.526 kb on - strand at 3334.565 kb on - strand at 3334.566 kb on + strand at 3334.567 kb on - strand at 3334.573 kb on - strand at 3334.583 kb on + strand at 3334.625 kb on + strand, within RR41_RS16065 at 3334.666 kb on + strand, within RR41_RS16065 at 3334.671 kb on + strand, within RR41_RS16065 at 3334.671 kb on + strand, within RR41_RS16065 at 3334.671 kb on + strand, within RR41_RS16065 at 3334.672 kb on - strand, within RR41_RS16065 at 3334.698 kb on + strand, within RR41_RS16065 at 3334.698 kb on + strand, within RR41_RS16065 at 3334.707 kb on + strand, within RR41_RS16065 at 3334.725 kb on + strand, within RR41_RS16065 at 3334.725 kb on + strand, within RR41_RS16065 at 3334.725 kb on + strand, within RR41_RS16065 at 3334.726 kb on - strand, within RR41_RS16065 at 3334.726 kb on - strand, within RR41_RS16065 at 3334.757 kb on + strand, within RR41_RS16065 at 3334.758 kb on - strand, within RR41_RS16065 at 3334.776 kb on - strand, within RR41_RS16065 at 3334.790 kb on + strand, within RR41_RS16065 at 3334.799 kb on + strand, within RR41_RS16065 at 3334.814 kb on + strand, within RR41_RS16065 at 3334.814 kb on + strand, within RR41_RS16065 at 3334.814 kb on + strand, within RR41_RS16065 at 3334.815 kb on - strand, within RR41_RS16065 at 3334.815 kb on - strand, within RR41_RS16065 at 3334.837 kb on + strand, within RR41_RS16065 at 3334.837 kb on + strand, within RR41_RS16065 at 3334.837 kb on + strand, within RR41_RS16065 at 3334.837 kb on + strand, within RR41_RS16065 at 3334.837 kb on + strand, within RR41_RS16065 at 3334.838 kb on - strand, within RR41_RS16065 at 3334.892 kb on - strand, within RR41_RS16065 at 3334.892 kb on - strand, within RR41_RS16065 at 3334.966 kb on + strand at 3334.966 kb on + strand at 3334.994 kb on - strand at 3335.068 kb on + strand, within RR41_RS16070 at 3335.109 kb on + strand, within RR41_RS16070 at 3335.109 kb on + strand, within RR41_RS16070 at 3335.110 kb on - strand, within RR41_RS16070 at 3335.110 kb on - strand, within RR41_RS16070 at 3335.113 kb on + strand, within RR41_RS16070 at 3335.113 kb on + strand, within RR41_RS16070 at 3335.161 kb on + strand, within RR41_RS16070 at 3335.161 kb on + strand, within RR41_RS16070 at 3335.162 kb on - strand, within RR41_RS16070 at 3335.162 kb on - strand, within RR41_RS16070 at 3335.162 kb on - strand, within RR41_RS16070 at 3335.162 kb on - strand, within RR41_RS16070 at 3335.358 kb on + strand, within RR41_RS16070 at 3335.359 kb on - strand, within RR41_RS16070 at 3335.359 kb on - strand, within RR41_RS16070 at 3335.368 kb on + strand, within RR41_RS16070 at 3335.369 kb on - strand, within RR41_RS16070 at 3335.369 kb on - strand, within RR41_RS16070 at 3335.428 kb on + strand, within RR41_RS16070 at 3335.428 kb on + strand, within RR41_RS16070 at 3335.428 kb on + strand, within RR41_RS16070 at 3335.428 kb on + strand, within RR41_RS16070 at 3335.429 kb on - strand, within RR41_RS16070 at 3335.429 kb on - strand, within RR41_RS16070
Per-strain Table
Position Strand Gene LocusTag Fraction MHII_biocarbonate_serum_Chelated; 35C remove 3,331,994 + RR41_RS16050 0.44 -1.6 3,332,052 + RR41_RS16050 0.51 +1.4 3,332,439 + +1.4 3,332,482 + -0.6 3,332,482 + -1.8 3,332,483 - +0.8 3,332,485 + +1.7 3,332,486 - +1.4 3,332,497 + -1.1 3,332,521 - -0.9 3,333,098 - +0.1 3,333,098 - -0.9 3,333,244 + RR41_RS16060 0.18 -0.2 3,333,245 - RR41_RS16060 0.18 -2.4 3,333,245 - RR41_RS16060 0.18 -1.2 3,333,245 - RR41_RS16060 0.18 +1.9 3,333,245 - RR41_RS16060 0.18 -0.9 3,333,245 - RR41_RS16060 0.18 -1.8 3,333,247 - RR41_RS16060 0.19 +0.4 3,333,247 - RR41_RS16060 0.19 -0.0 3,333,350 + RR41_RS16060 0.26 -0.9 3,333,350 + RR41_RS16060 0.26 -0.6 3,333,351 - RR41_RS16060 0.26 -1.8 3,333,351 - RR41_RS16060 0.26 +0.2 3,333,351 - RR41_RS16060 0.26 -0.8 3,333,351 - RR41_RS16060 0.26 +0.3 3,333,353 + RR41_RS16060 0.26 -0.6 3,333,354 - RR41_RS16060 0.26 -0.2 3,333,354 - RR41_RS16060 0.26 +1.0 3,333,354 - RR41_RS16060 0.26 -2.0 3,333,458 - RR41_RS16060 0.33 -0.6 3,333,481 + RR41_RS16060 0.35 -1.6 3,333,481 + RR41_RS16060 0.35 -2.2 3,333,482 - RR41_RS16060 0.35 -2.1 3,333,482 - RR41_RS16060 0.35 -2.7 3,333,482 - RR41_RS16060 0.35 -0.5 3,333,560 + RR41_RS16060 0.40 -0.6 3,333,609 + RR41_RS16060 0.43 -1.4 3,333,610 - RR41_RS16060 0.43 -0.6 3,333,659 - RR41_RS16060 0.47 +0.1 3,333,679 + RR41_RS16060 0.48 -0.2 3,333,749 + RR41_RS16060 0.53 -1.8 3,333,750 - RR41_RS16060 0.53 -0.6 3,333,752 + RR41_RS16060 0.53 -1.6 3,333,752 + RR41_RS16060 0.53 -1.9 3,333,753 - RR41_RS16060 0.53 -0.6 3,333,753 - RR41_RS16060 0.53 -1.3 3,333,799 - RR41_RS16060 0.56 +1.1 3,333,799 - RR41_RS16060 0.56 -1.8 3,333,799 - RR41_RS16060 0.56 -0.9 3,333,799 - RR41_RS16060 0.56 +0.4 3,333,799 - RR41_RS16060 0.56 +0.8 3,333,799 - RR41_RS16060 0.56 -1.8 3,333,827 + RR41_RS16060 0.58 -1.2 3,333,890 - RR41_RS16060 0.62 -0.6 3,333,890 - RR41_RS16060 0.62 -0.2 3,333,903 - RR41_RS16060 0.63 -0.9 3,334,016 - RR41_RS16060 0.71 +0.1 3,334,016 - RR41_RS16060 0.71 -1.4 3,334,033 - RR41_RS16060 0.72 -1.6 3,334,036 - RR41_RS16060 0.72 -0.6 3,334,036 - RR41_RS16060 0.72 -0.6 3,334,043 - RR41_RS16060 0.73 -0.6 3,334,186 - RR41_RS16060 0.83 -1.6 3,334,301 + -0.2 3,334,302 - +1.4 3,334,307 + +0.7 3,334,308 - -0.6 3,334,334 - -0.6 3,334,334 - -0.2 3,334,334 - -1.4 3,334,334 - -2.2 3,334,336 - -1.2 3,334,336 - -1.8 3,334,454 - +0.4 3,334,454 - -0.2 3,334,466 - +1.4 3,334,466 - -2.3 3,334,469 + -2.1 3,334,470 - +1.4 3,334,489 + +1.4 3,334,489 + +0.8 3,334,489 + -0.6 3,334,489 + +0.1 3,334,490 - -2.6 3,334,490 - +1.4 3,334,490 - +1.4 3,334,490 - -0.1 3,334,495 + -1.3 3,334,496 - -0.7 3,334,526 - -1.7 3,334,565 - -1.2 3,334,566 + -1.1 3,334,567 - -1.4 3,334,573 - -0.2 3,334,583 + -0.9 3,334,625 + RR41_RS16065 0.15 -1.9 3,334,666 + RR41_RS16065 0.25 -0.2 3,334,671 + RR41_RS16065 0.26 -0.8 3,334,671 + RR41_RS16065 0.26 -0.2 3,334,671 + RR41_RS16065 0.26 -1.8 3,334,672 - RR41_RS16065 0.27 +2.4 3,334,698 + RR41_RS16065 0.33 +2.4 3,334,698 + RR41_RS16065 0.33 +1.0 3,334,707 + RR41_RS16065 0.35 +0.1 3,334,725 + RR41_RS16065 0.39 +0.1 3,334,725 + RR41_RS16065 0.39 -2.1 3,334,725 + RR41_RS16065 0.39 -0.2 3,334,726 - RR41_RS16065 0.40 -0.8 3,334,726 - RR41_RS16065 0.40 +2.0 3,334,757 + RR41_RS16065 0.47 -0.2 3,334,758 - RR41_RS16065 0.47 +1.1 3,334,776 - RR41_RS16065 0.52 -0.8 3,334,790 + RR41_RS16065 0.55 -0.6 3,334,799 + RR41_RS16065 0.57 -2.2 3,334,814 + RR41_RS16065 0.61 -0.7 3,334,814 + RR41_RS16065 0.61 -0.9 3,334,814 + RR41_RS16065 0.61 -1.2 3,334,815 - RR41_RS16065 0.61 -0.9 3,334,815 - RR41_RS16065 0.61 -0.6 3,334,837 + RR41_RS16065 0.67 -1.2 3,334,837 + RR41_RS16065 0.67 +0.3 3,334,837 + RR41_RS16065 0.67 -1.4 3,334,837 + RR41_RS16065 0.67 +0.8 3,334,837 + RR41_RS16065 0.67 -0.6 3,334,838 - RR41_RS16065 0.67 +1.4 3,334,892 - RR41_RS16065 0.80 +1.4 3,334,892 - RR41_RS16065 0.80 -0.6 3,334,966 + +2.2 3,334,966 + +0.1 3,334,994 - -1.2 3,335,068 + RR41_RS16070 0.16 -1.9 3,335,109 + RR41_RS16070 0.21 -1.4 3,335,109 + RR41_RS16070 0.21 +2.4 3,335,110 - RR41_RS16070 0.21 -1.4 3,335,110 - RR41_RS16070 0.21 +1.0 3,335,113 + RR41_RS16070 0.21 -3.5 3,335,113 + RR41_RS16070 0.21 +2.0 3,335,161 + RR41_RS16070 0.27 +0.2 3,335,161 + RR41_RS16070 0.27 +0.4 3,335,162 - RR41_RS16070 0.27 -2.3 3,335,162 - RR41_RS16070 0.27 -0.6 3,335,162 - RR41_RS16070 0.27 -0.6 3,335,162 - RR41_RS16070 0.27 +0.4 3,335,358 + RR41_RS16070 0.52 +0.4 3,335,359 - RR41_RS16070 0.52 -0.8 3,335,359 - RR41_RS16070 0.52 +0.4 3,335,368 + RR41_RS16070 0.53 +1.0 3,335,369 - RR41_RS16070 0.53 +2.4 3,335,369 - RR41_RS16070 0.53 -0.3 3,335,428 + RR41_RS16070 0.60 -0.6 3,335,428 + RR41_RS16070 0.60 -0.4 3,335,428 + RR41_RS16070 0.60 -1.6 3,335,428 + RR41_RS16070 0.60 +0.5 3,335,429 - RR41_RS16070 0.60 -0.6 3,335,429 - RR41_RS16070 0.60 +0.7
Or see this region's nucleotide sequence