Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF72 and GFF73 are separated by 298 nucleotides GFF73 and GFF74 are separated by 124 nucleotides GFF74 and GFF75 are separated by 83 nucleotides GFF75 and GFF76 are separated by 45 nucleotides
GFF72 - Tol-Pal system protein TolQ, at 82,131 to 82,937
GFF72
GFF73 - hypothetical protein, at 83,236 to 83,745
GFF73
GFF74 - hypothetical protein, at 83,870 to 84,277
GFF74
GFF75 - hypothetical protein, at 84,361 to 84,993
GFF75
GFF76 - Holliday junction ATP-dependent DNA helicase RuvB, at 85,039 to 86,070
GFF76
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 82.997 kb on - strand at 83.180 kb on + strand at 83.180 kb on + strand at 83.181 kb on - strand at 83.606 kb on + strand, within GFF73 at 83.606 kb on + strand, within GFF73 at 83.606 kb on + strand, within GFF73 at 83.607 kb on - strand, within GFF73 at 83.607 kb on - strand, within GFF73 at 83.713 kb on - strand at 84.034 kb on - strand, within GFF74 at 84.250 kb on - strand at 84.265 kb on - strand at 84.265 kb on - strand at 84.319 kb on - strand at 84.392 kb on + strand at 84.959 kb on + strand at 84.960 kb on - strand at 84.960 kb on - strand at 85.006 kb on + strand at 85.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 82,997 - +0.0 83,180 + +0.3 83,180 + +2.1 83,181 - +0.2 83,606 + GFF73 0.73 -0.8 83,606 + GFF73 0.73 +0.1 83,606 + GFF73 0.73 -2.5 83,607 - GFF73 0.73 +0.0 83,607 - GFF73 0.73 +0.0 83,713 - +0.1 84,034 - GFF74 0.40 -0.6 84,250 - -0.9 84,265 - -0.2 84,265 - -2.0 84,319 - -0.6 84,392 + -0.0 84,959 + -0.2 84,960 - +0.4 84,960 - -1.3 85,006 + -0.0 85,007 - -0.5
Or see this region's nucleotide sequence