Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF722 and GFF723 are separated by 145 nucleotides GFF723 and GFF724 are separated by 112 nucleotides GFF724 and GFF725 overlap by 8 nucleotides
GFF722 - Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, at 769,736 to 771,103
GFF722
GFF723 - hypothetical protein, at 771,249 to 771,485
GFF723
GFF724 - hypothetical protein, at 771,598 to 772,233
GFF724
GFF725 - Dihydrolipoyl dehydrogenase, at 772,226 to 773,641
GFF725
Position (kb)
771
772
773 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 770.730 kb on - strand, within GFF722 at 770.838 kb on - strand, within GFF722 at 771.122 kb on - strand at 771.197 kb on + strand at 771.291 kb on + strand, within GFF723 at 771.354 kb on + strand, within GFF723 at 771.354 kb on + strand, within GFF723 at 771.355 kb on - strand, within GFF723 at 771.355 kb on - strand, within GFF723 at 771.466 kb on - strand at 771.466 kb on - strand at 771.466 kb on - strand at 771.898 kb on + strand, within GFF724 at 771.899 kb on - strand, within GFF724 at 772.062 kb on - strand, within GFF724 at 772.234 kb on + strand at 772.234 kb on + strand at 772.234 kb on + strand at 772.235 kb on - strand at 772.235 kb on - strand at 772.238 kb on + strand at 772.239 kb on - strand at 772.274 kb on + strand at 772.404 kb on - strand, within GFF725 at 772.409 kb on + strand, within GFF725 at 772.409 kb on + strand, within GFF725 at 772.410 kb on - strand, within GFF725 at 772.430 kb on + strand, within GFF725 at 772.643 kb on + strand, within GFF725 at 772.644 kb on - strand, within GFF725 at 772.730 kb on + strand, within GFF725 at 772.817 kb on + strand, within GFF725
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 770,730 - GFF722 0.73 -1.1 770,838 - GFF722 0.81 +1.2 771,122 - +1.2 771,197 + -0.3 771,291 + GFF723 0.18 -0.1 771,354 + GFF723 0.44 +0.7 771,354 + GFF723 0.44 -0.3 771,355 - GFF723 0.45 +0.1 771,355 - GFF723 0.45 -1.1 771,466 - -0.2 771,466 - +1.1 771,466 - +0.2 771,898 + GFF724 0.47 +0.1 771,899 - GFF724 0.47 -1.0 772,062 - GFF724 0.73 -2.5 772,234 + +0.6 772,234 + +0.2 772,234 + +0.3 772,235 - -0.8 772,235 - +1.1 772,238 + +0.5 772,239 - -0.2 772,274 + +0.6 772,404 - GFF725 0.13 +0.0 772,409 + GFF725 0.13 +0.5 772,409 + GFF725 0.13 +1.2 772,410 - GFF725 0.13 +1.9 772,430 + GFF725 0.14 -0.5 772,643 + GFF725 0.29 -0.4 772,644 - GFF725 0.30 +0.2 772,730 + GFF725 0.36 +2.5 772,817 + GFF725 0.42 +0.2
Or see this region's nucleotide sequence