Strain Fitness in Xanthobacter sp. DMC5 around GFF66

Experiment: D,L-Lactate and casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF64 and GFF65 are separated by 18 nucleotidesGFF65 and GFF66 are separated by 9 nucleotidesGFF66 and GFF67 are separated by 198 nucleotides GFF64 - Adenine phosphoribosyltransferase, at 73,982 to 74,518 GFF64 GFF65 - hypothetical protein, at 74,537 to 75,241 GFF65 GFF66 - Methionine aminopeptidase, at 75,251 to 76,078 GFF66 GFF67 - DNA mismatch repair protein MutL, at 76,277 to 78,124 GFF67 Position (kb) 75 76 77Strain fitness (log2 ratio) -2 -1 0 1 2 3at 74.754 kb on + strand, within GFF65at 74.856 kb on + strand, within GFF65at 74.857 kb on - strand, within GFF65at 74.877 kb on + strand, within GFF65at 74.877 kb on + strand, within GFF65at 74.921 kb on + strand, within GFF65at 75.264 kb on + strandat 75.264 kb on + strandat 75.264 kb on + strandat 75.264 kb on + strandat 75.264 kb on + strandat 75.264 kb on + strandat 75.265 kb on - strandat 75.279 kb on + strandat 75.654 kb on + strand, within GFF66at 75.654 kb on + strand, within GFF66at 75.855 kb on + strand, within GFF66at 75.873 kb on + strand, within GFF66at 76.071 kb on + strandat 76.133 kb on + strandat 76.134 kb on - strandat 76.141 kb on + strandat 76.141 kb on + strandat 76.141 kb on + strandat 76.142 kb on - strandat 76.142 kb on - strandat 76.142 kb on - strandat 76.245 kb on - strandat 76.725 kb on - strand, within GFF67at 77.033 kb on + strand, within GFF67at 77.033 kb on + strand, within GFF67at 77.034 kb on - strand, within GFF67at 77.034 kb on - strand, within GFF67at 77.034 kb on - strand, within GFF67

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C)
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74,754 + GFF65 0.31 -0.1
74,856 + GFF65 0.45 +0.6
74,857 - GFF65 0.45 -1.0
74,877 + GFF65 0.48 -0.1
74,877 + GFF65 0.48 +1.2
74,921 + GFF65 0.54 -0.5
75,264 + +0.5
75,264 + +0.8
75,264 + +0.4
75,264 + +0.4
75,264 + -1.1
75,264 + -0.7
75,265 - -0.1
75,279 + +1.5
75,654 + GFF66 0.49 -1.1
75,654 + GFF66 0.49 +0.4
75,855 + GFF66 0.73 +0.2
75,873 + GFF66 0.75 -2.1
76,071 + -0.4
76,133 + +2.8
76,134 - -1.2
76,141 + -0.3
76,141 + -0.7
76,141 + +0.3
76,142 - -1.8
76,142 - -1.0
76,142 - +0.9
76,245 - -1.0
76,725 - GFF67 0.24 +0.9
77,033 + GFF67 0.41 -0.0
77,033 + GFF67 0.41 +0.5
77,034 - GFF67 0.41 +0.8
77,034 - GFF67 0.41 +0.4
77,034 - GFF67 0.41 +1.0

Or see this region's nucleotide sequence