Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4721 and GFF4722 are separated by 97 nucleotides GFF4722 and GFF4723 are separated by 97 nucleotides GFF4723 and GFF4724 are separated by 3 nucleotides GFF4724 and GFF4725 are separated by 46 nucleotides
GFF4721 - hypothetical protein, at 132,716 to 133,117
GFF4721
GFF4722 - 50S ribosomal protein L31, at 133,215 to 133,439
GFF4722
GFF4723 - Multidrug resistance ABC transporter ATP-binding/permease protein BmrA, at 133,537 to 135,351
GFF4723
GFF4724 - hypothetical protein, at 135,355 to 135,885
GFF4724
GFF4725 - Bifunctional transcriptional activator/DNA repair enzyme Ada, at 135,932 to 137,011
GFF4725
Position (kb) 133
134
135
136 Strain fitness (log2 ratio) -1
0
1
2
3 at 132.644 kb on + strand at 132.645 kb on - strand at 132.645 kb on - strand at 132.651 kb on + strand at 132.652 kb on - strand at 132.652 kb on - strand at 132.652 kb on - strand at 132.664 kb on + strand at 133.454 kb on - strand at 133.520 kb on - strand at 133.643 kb on - strand at 133.786 kb on + strand, within GFF4723 at 133.934 kb on - strand, within GFF4723 at 134.204 kb on - strand, within GFF4723 at 134.455 kb on + strand, within GFF4723 at 134.455 kb on + strand, within GFF4723 at 134.648 kb on - strand, within GFF4723 at 134.777 kb on - strand, within GFF4723 at 135.311 kb on - strand at 135.758 kb on - strand, within GFF4724 at 135.758 kb on - strand, within GFF4724 at 135.804 kb on + strand, within GFF4724 at 135.805 kb on - strand, within GFF4724 at 135.806 kb on + strand, within GFF4724 at 135.807 kb on - strand, within GFF4724 at 135.911 kb on + strand at 135.911 kb on + strand at 135.912 kb on - strand at 135.912 kb on - strand at 136.119 kb on + strand, within GFF4725 at 136.270 kb on + strand, within GFF4725 at 136.271 kb on - strand, within GFF4725
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 132,644 + -1.0 132,645 - -0.6 132,645 - -0.3 132,651 + -0.1 132,652 - -0.8 132,652 - -0.5 132,652 - -1.1 132,664 + -1.6 133,454 - +1.2 133,520 - +0.7 133,643 - -0.4 133,786 + GFF4723 0.14 -1.5 133,934 - GFF4723 0.22 +0.2 134,204 - GFF4723 0.37 -0.4 134,455 + GFF4723 0.51 -0.3 134,455 + GFF4723 0.51 -0.8 134,648 - GFF4723 0.61 -0.6 134,777 - GFF4723 0.68 -1.2 135,311 - +1.8 135,758 - GFF4724 0.76 +3.0 135,758 - GFF4724 0.76 +2.2 135,804 + GFF4724 0.85 +1.9 135,805 - GFF4724 0.85 +0.8 135,806 + GFF4724 0.85 +3.0 135,807 - GFF4724 0.85 +2.1 135,911 + +0.9 135,911 + -0.9 135,912 - +0.3 135,912 - -1.1 136,119 + GFF4725 0.17 +1.2 136,270 + GFF4725 0.31 -0.2 136,271 - GFF4725 0.31 -0.4
Or see this region's nucleotide sequence