Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF399 and GFF400 are separated by 118 nucleotides GFF400 and GFF401 overlap by 4 nucleotides GFF401 and GFF402 overlap by 4 nucleotides GFF402 and GFF403 are separated by 6 nucleotides
GFF399 - hypothetical protein, at 411,787 to 413,106
GFF399
GFF400 - Peptidoglycan-associated lipoprotein, at 413,225 to 415,441
GFF400
GFF401 - hypothetical protein, at 415,438 to 415,542
GFF401
GFF402 - hypothetical protein, at 415,539 to 416,174
GFF402
GFF403 - Membrane-bound lytic murein transglycosylase C, at 416,181 to 418,478
GFF403
Position (kb)
413
414
415
416 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 412.237 kb on + strand, within GFF399 at 412.237 kb on + strand, within GFF399 at 412.237 kb on + strand, within GFF399 at 412.363 kb on + strand, within GFF399 at 412.435 kb on - strand, within GFF399 at 412.468 kb on + strand, within GFF399 at 412.519 kb on + strand, within GFF399 at 412.519 kb on + strand, within GFF399 at 412.520 kb on - strand, within GFF399 at 412.621 kb on + strand, within GFF399 at 412.621 kb on + strand, within GFF399 at 412.621 kb on + strand, within GFF399 at 412.674 kb on + strand, within GFF399 at 412.675 kb on - strand, within GFF399 at 412.675 kb on - strand, within GFF399 at 412.696 kb on + strand, within GFF399 at 412.696 kb on + strand, within GFF399 at 412.698 kb on + strand, within GFF399 at 412.698 kb on + strand, within GFF399 at 412.699 kb on - strand, within GFF399 at 412.718 kb on - strand, within GFF399 at 412.747 kb on + strand, within GFF399 at 412.870 kb on + strand, within GFF399 at 412.880 kb on - strand, within GFF399 at 412.886 kb on + strand, within GFF399 at 412.890 kb on + strand, within GFF399 at 412.890 kb on + strand, within GFF399 at 412.912 kb on + strand, within GFF399 at 412.912 kb on + strand, within GFF399 at 412.913 kb on - strand, within GFF399 at 412.937 kb on - strand, within GFF399 at 412.952 kb on - strand, within GFF399 at 412.997 kb on - strand at 413.009 kb on - strand at 413.026 kb on + strand at 413.027 kb on - strand at 413.027 kb on - strand at 413.135 kb on + strand at 413.135 kb on + strand at 413.135 kb on + strand at 413.136 kb on - strand at 413.178 kb on + strand at 413.178 kb on + strand at 413.179 kb on - strand at 413.179 kb on - strand at 413.228 kb on + strand at 413.229 kb on - strand at 413.648 kb on + strand, within GFF400 at 413.648 kb on + strand, within GFF400 at 413.877 kb on - strand, within GFF400 at 414.015 kb on - strand, within GFF400 at 414.015 kb on - strand, within GFF400 at 414.612 kb on - strand, within GFF400 at 415.174 kb on + strand, within GFF400 at 415.444 kb on + strand at 415.444 kb on + strand at 415.468 kb on + strand, within GFF401 at 415.468 kb on + strand, within GFF401 at 415.468 kb on + strand, within GFF401 at 415.468 kb on + strand, within GFF401 at 415.469 kb on - strand, within GFF401 at 415.469 kb on - strand, within GFF401 at 415.603 kb on + strand, within GFF402 at 415.604 kb on - strand, within GFF402 at 416.077 kb on - strand, within GFF402 at 416.077 kb on - strand, within GFF402 at 416.133 kb on - strand at 416.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 412,237 + GFF399 0.34 +0.5 412,237 + GFF399 0.34 -0.7 412,237 + GFF399 0.34 -0.1 412,363 + GFF399 0.44 +0.7 412,435 - GFF399 0.49 +0.8 412,468 + GFF399 0.52 -0.5 412,519 + GFF399 0.55 -0.4 412,519 + GFF399 0.55 -2.2 412,520 - GFF399 0.56 -0.2 412,621 + GFF399 0.63 -0.6 412,621 + GFF399 0.63 +0.2 412,621 + GFF399 0.63 -0.2 412,674 + GFF399 0.67 -0.2 412,675 - GFF399 0.67 +0.6 412,675 - GFF399 0.67 +0.8 412,696 + GFF399 0.69 -0.5 412,696 + GFF399 0.69 +0.1 412,698 + GFF399 0.69 -0.3 412,698 + GFF399 0.69 -0.1 412,699 - GFF399 0.69 -0.9 412,718 - GFF399 0.71 +0.0 412,747 + GFF399 0.73 +1.1 412,870 + GFF399 0.82 -0.3 412,880 - GFF399 0.83 +0.1 412,886 + GFF399 0.83 -0.4 412,890 + GFF399 0.84 -0.2 412,890 + GFF399 0.84 +0.1 412,912 + GFF399 0.85 -0.5 412,912 + GFF399 0.85 +0.6 412,913 - GFF399 0.85 -0.6 412,937 - GFF399 0.87 +0.4 412,952 - GFF399 0.88 +0.4 412,997 - -0.5 413,009 - -0.9 413,026 + +0.3 413,027 - +0.7 413,027 - +0.1 413,135 + +0.1 413,135 + +1.1 413,135 + -0.9 413,136 - -0.5 413,178 + -0.1 413,178 + -0.4 413,179 - -2.0 413,179 - -0.7 413,228 + -0.3 413,229 - -0.4 413,648 + GFF400 0.19 +0.4 413,648 + GFF400 0.19 +0.4 413,877 - GFF400 0.29 -0.1 414,015 - GFF400 0.36 -0.1 414,015 - GFF400 0.36 -0.9 414,612 - GFF400 0.63 -1.6 415,174 + GFF400 0.88 -0.2 415,444 + +0.5 415,444 + -0.8 415,468 + GFF401 0.29 +1.1 415,468 + GFF401 0.29 -0.7 415,468 + GFF401 0.29 +0.6 415,468 + GFF401 0.29 -1.0 415,469 - GFF401 0.30 +0.9 415,469 - GFF401 0.30 +0.6 415,603 + GFF402 0.10 +0.2 415,604 - GFF402 0.10 -0.3 416,077 - GFF402 0.85 -2.8 416,077 - GFF402 0.85 -0.4 416,133 - +1.3 416,230 + +0.1
Or see this region's nucleotide sequence