Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF299 and GFF300 are separated by 3 nucleotides GFF300 and GFF301 overlap by 4 nucleotides GFF301 and GFF302 overlap by 4 nucleotides GFF302 and GFF303 are separated by 3 nucleotides GFF303 and GFF304 are separated by 1 nucleotides
GFF299 - Single-stranded DNA-binding protein, at 313,243 to 313,668
GFF299
GFF300 - ATP-dependent zinc metalloprotease FtsH, at 313,672 to 314,595
GFF300
GFF301 - hypothetical protein, at 314,592 to 315,494
GFF301
GFF302 - hypothetical protein, at 315,491 to 316,069
GFF302
GFF303 - hypothetical protein, at 316,073 to 316,333
GFF303
GFF304 - hypothetical protein, at 316,335 to 316,610
GFF304
Position (kb)
314
315
316 Strain fitness (log2 ratio)
-1
0
1 at 313.634 kb on + strand at 313.634 kb on + strand at 313.635 kb on - strand at 313.686 kb on + strand at 313.686 kb on + strand at 313.686 kb on + strand at 313.971 kb on - strand, within GFF300 at 313.971 kb on - strand, within GFF300 at 313.971 kb on - strand, within GFF300 at 314.119 kb on + strand, within GFF300 at 314.384 kb on + strand, within GFF300 at 314.384 kb on + strand, within GFF300 at 314.384 kb on + strand, within GFF300 at 314.385 kb on - strand, within GFF300 at 314.462 kb on + strand, within GFF300 at 314.463 kb on - strand, within GFF300 at 314.463 kb on - strand, within GFF300 at 314.463 kb on - strand, within GFF300 at 314.463 kb on - strand, within GFF300 at 314.463 kb on - strand, within GFF300 at 314.545 kb on - strand at 314.545 kb on - strand at 314.907 kb on + strand, within GFF301 at 314.907 kb on + strand, within GFF301 at 314.907 kb on + strand, within GFF301 at 315.084 kb on - strand, within GFF301 at 315.085 kb on + strand, within GFF301 at 315.086 kb on - strand, within GFF301 at 315.283 kb on + strand, within GFF301 at 315.283 kb on + strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.461 kb on - strand at 315.461 kb on - strand at 315.526 kb on + strand at 315.526 kb on + strand at 315.921 kb on + strand, within GFF302 at 315.921 kb on + strand, within GFF302 at 315.921 kb on + strand, within GFF302 at 316.017 kb on + strand at 316.017 kb on + strand at 316.018 kb on - strand at 316.260 kb on + strand, within GFF303
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 313,634 + +0.5 313,634 + +0.7 313,635 - +0.8 313,686 + +0.4 313,686 + +0.3 313,686 + -1.0 313,971 - GFF300 0.32 -0.6 313,971 - GFF300 0.32 -0.3 313,971 - GFF300 0.32 -0.1 314,119 + GFF300 0.48 -1.5 314,384 + GFF300 0.77 +0.4 314,384 + GFF300 0.77 -1.7 314,384 + GFF300 0.77 +0.4 314,385 - GFF300 0.77 -0.2 314,462 + GFF300 0.85 +1.1 314,463 - GFF300 0.86 -0.1 314,463 - GFF300 0.86 -0.0 314,463 - GFF300 0.86 -0.0 314,463 - GFF300 0.86 -0.6 314,463 - GFF300 0.86 -0.0 314,545 - -0.5 314,545 - +0.1 314,907 + GFF301 0.35 +0.5 314,907 + GFF301 0.35 +0.7 314,907 + GFF301 0.35 -0.9 315,084 - GFF301 0.54 +0.3 315,085 + GFF301 0.55 -0.9 315,086 - GFF301 0.55 -0.6 315,283 + GFF301 0.77 +0.3 315,283 + GFF301 0.77 +0.8 315,284 - GFF301 0.77 +0.3 315,284 - GFF301 0.77 -0.8 315,284 - GFF301 0.77 +0.3 315,284 - GFF301 0.77 -0.2 315,461 - -1.4 315,461 - +0.2 315,526 + +1.0 315,526 + -0.8 315,921 + GFF302 0.74 +0.5 315,921 + GFF302 0.74 +1.0 315,921 + GFF302 0.74 +0.4 316,017 + -0.4 316,017 + -0.2 316,018 - +0.4 316,260 + GFF303 0.72 +0.2
Or see this region's nucleotide sequence