Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF24 and GFF25 are separated by 29 nucleotides GFF25 and GFF26 overlap by 4 nucleotides GFF26 and GFF27 overlap by 34 nucleotides GFF27 and GFF28 are separated by 54 nucleotides
GFF24 - FMNH(2)-dependent dimethylsulfone monooxygenase, at 29,401 to 30,564
GFF24
GFF25 - Flavin reductase, at 30,594 to 31,109
GFF25
GFF26 - Acetyl-CoA:oxalate CoA-transferase, at 31,106 to 32,308
GFF26
GFF27 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 32,275 to 33,018
GFF27
GFF28 - HTH-type transcriptional repressor RspR, at 33,073 to 33,801
GFF28
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-1
0
1 at 30.147 kb on + strand, within GFF24 at 30.215 kb on + strand, within GFF24 at 30.254 kb on - strand, within GFF24 at 30.254 kb on - strand, within GFF24 at 30.301 kb on + strand, within GFF24 at 30.574 kb on + strand at 30.762 kb on + strand, within GFF25 at 30.763 kb on - strand, within GFF25 at 30.763 kb on - strand, within GFF25 at 31.059 kb on + strand at 31.060 kb on - strand at 31.060 kb on - strand at 31.075 kb on - strand at 31.075 kb on - strand at 31.075 kb on - strand at 31.502 kb on + strand, within GFF26 at 31.502 kb on + strand, within GFF26 at 31.503 kb on - strand, within GFF26 at 31.539 kb on - strand, within GFF26 at 31.539 kb on - strand, within GFF26 at 31.664 kb on + strand, within GFF26 at 31.725 kb on - strand, within GFF26 at 31.725 kb on - strand, within GFF26 at 31.762 kb on - strand, within GFF26 at 31.784 kb on + strand, within GFF26 at 31.844 kb on + strand, within GFF26 at 31.948 kb on + strand, within GFF26 at 32.183 kb on + strand, within GFF26 at 32.184 kb on + strand, within GFF26 at 32.185 kb on - strand, within GFF26 at 32.642 kb on - strand, within GFF27 at 32.642 kb on - strand, within GFF27 at 32.995 kb on + strand at 32.996 kb on - strand at 33.033 kb on + strand at 33.033 kb on + strand at 33.034 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 30,147 + GFF24 0.64 +0.2 30,215 + GFF24 0.70 -0.1 30,254 - GFF24 0.73 -0.7 30,254 - GFF24 0.73 -0.1 30,301 + GFF24 0.77 +1.3 30,574 + +0.4 30,762 + GFF25 0.33 -0.2 30,763 - GFF25 0.33 +0.0 30,763 - GFF25 0.33 +0.5 31,059 + +1.2 31,060 - -0.5 31,060 - -0.5 31,075 - -0.5 31,075 - -0.2 31,075 - -0.3 31,502 + GFF26 0.33 -0.3 31,502 + GFF26 0.33 -0.9 31,503 - GFF26 0.33 -0.2 31,539 - GFF26 0.36 -1.0 31,539 - GFF26 0.36 -1.6 31,664 + GFF26 0.46 +0.3 31,725 - GFF26 0.51 +0.4 31,725 - GFF26 0.51 +1.3 31,762 - GFF26 0.55 +0.4 31,784 + GFF26 0.56 +1.5 31,844 + GFF26 0.61 +0.2 31,948 + GFF26 0.70 -1.2 32,183 + GFF26 0.90 +1.1 32,184 + GFF26 0.90 -0.5 32,185 - GFF26 0.90 -0.2 32,642 - GFF27 0.49 -0.2 32,642 - GFF27 0.49 +0.1 32,995 + +1.0 32,996 - +0.9 33,033 + +0.1 33,033 + -0.1 33,034 - -0.2
Or see this region's nucleotide sequence