Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF251 and GFF252 are separated by 123 nucleotides GFF252 and GFF253 are separated by 195 nucleotides GFF253 and GFF254 are separated by 42 nucleotides
GFF251 - Protein-methionine-sulfoxide reductase heme-binding subunit MsrQ, at 265,033 to 265,896
GFF251
GFF252 - hypothetical protein, at 266,020 to 267,132
GFF252
GFF253 - 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxonon atrienedioate hydrolase, at 267,328 to 267,927
GFF253
GFF254 - Thymidylate synthase, at 267,970 to 268,764
GFF254
Position (kb) 266
267
268 Strain fitness (log2 ratio) -2
-1
0
1 at 265.054 kb on - strand at 265.054 kb on - strand at 265.054 kb on - strand at 265.054 kb on - strand at 265.135 kb on + strand, within GFF251 at 265.135 kb on + strand, within GFF251 at 265.135 kb on + strand, within GFF251 at 265.135 kb on + strand, within GFF251 at 265.136 kb on - strand, within GFF251 at 265.136 kb on - strand, within GFF251 at 265.136 kb on - strand, within GFF251 at 265.225 kb on + strand, within GFF251 at 265.225 kb on + strand, within GFF251 at 265.226 kb on - strand, within GFF251 at 265.226 kb on - strand, within GFF251 at 265.226 kb on - strand, within GFF251 at 265.226 kb on - strand, within GFF251 at 265.229 kb on + strand, within GFF251 at 265.500 kb on + strand, within GFF251 at 265.550 kb on + strand, within GFF251 at 265.550 kb on + strand, within GFF251 at 265.550 kb on + strand, within GFF251 at 265.551 kb on - strand, within GFF251 at 265.551 kb on - strand, within GFF251 at 265.551 kb on - strand, within GFF251 at 265.597 kb on + strand, within GFF251 at 265.604 kb on + strand, within GFF251 at 265.605 kb on - strand, within GFF251 at 265.605 kb on - strand, within GFF251 at 265.667 kb on + strand, within GFF251 at 266.096 kb on + strand at 266.096 kb on + strand at 266.097 kb on - strand at 266.097 kb on - strand at 266.097 kb on - strand at 266.148 kb on - strand, within GFF252 at 266.501 kb on + strand, within GFF252 at 266.501 kb on + strand, within GFF252 at 266.501 kb on + strand, within GFF252 at 266.502 kb on - strand, within GFF252 at 266.502 kb on - strand, within GFF252 at 266.604 kb on - strand, within GFF252 at 266.604 kb on - strand, within GFF252 at 267.190 kb on + strand at 267.191 kb on - strand at 267.199 kb on - strand at 267.199 kb on - strand at 267.199 kb on - strand at 267.201 kb on + strand at 267.201 kb on + strand at 267.201 kb on + strand at 267.202 kb on - strand at 267.230 kb on + strand at 267.231 kb on - strand at 267.271 kb on - strand at 267.271 kb on - strand at 267.329 kb on + strand at 267.330 kb on - strand at 267.593 kb on + strand, within GFF253 at 267.711 kb on - strand, within GFF253 at 267.711 kb on - strand, within GFF253 at 267.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 265,054 - +0.3 265,054 - -2.1 265,054 - +1.3 265,054 - -0.3 265,135 + GFF251 0.12 +0.5 265,135 + GFF251 0.12 -2.1 265,135 + GFF251 0.12 +0.5 265,135 + GFF251 0.12 +0.3 265,136 - GFF251 0.12 -0.1 265,136 - GFF251 0.12 -0.1 265,136 - GFF251 0.12 +0.6 265,225 + GFF251 0.22 -1.4 265,225 + GFF251 0.22 +0.2 265,226 - GFF251 0.22 -0.5 265,226 - GFF251 0.22 +0.0 265,226 - GFF251 0.22 -0.4 265,226 - GFF251 0.22 +0.7 265,229 + GFF251 0.23 -0.4 265,500 + GFF251 0.54 -0.8 265,550 + GFF251 0.60 -0.5 265,550 + GFF251 0.60 +1.6 265,550 + GFF251 0.60 +0.8 265,551 - GFF251 0.60 -1.4 265,551 - GFF251 0.60 -0.4 265,551 - GFF251 0.60 -1.2 265,597 + GFF251 0.65 -0.2 265,604 + GFF251 0.66 -0.5 265,605 - GFF251 0.66 +0.5 265,605 - GFF251 0.66 -0.6 265,667 + GFF251 0.73 -0.7 266,096 + -0.4 266,096 + -1.0 266,097 - -2.1 266,097 - -0.5 266,097 - -0.2 266,148 - GFF252 0.12 +0.1 266,501 + GFF252 0.43 +0.6 266,501 + GFF252 0.43 -0.5 266,501 + GFF252 0.43 -0.3 266,502 - GFF252 0.43 -0.6 266,502 - GFF252 0.43 -1.5 266,604 - GFF252 0.52 +0.2 266,604 - GFF252 0.52 -1.3 267,190 + +0.5 267,191 - -1.1 267,199 - +0.5 267,199 - -0.5 267,199 - -0.4 267,201 + +0.8 267,201 + +0.9 267,201 + +0.7 267,202 - -0.7 267,230 + -1.3 267,231 - -0.3 267,271 - +0.7 267,271 - -0.1 267,329 + -1.6 267,330 - -0.2 267,593 + GFF253 0.44 -0.0 267,711 - GFF253 0.64 -0.2 267,711 - GFF253 0.64 -0.6 267,972 - -0.7
Or see this region's nucleotide sequence