Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2412 and GFF2413 are separated by 168 nucleotides GFF2413 and GFF2414 are separated by 34 nucleotides GFF2414 and GFF2415 are separated by 3 nucleotides GFF2415 and GFF2416 overlap by 4 nucleotides
GFF2412 - Putative non-heme bromoperoxidase BpoC, at 69,470 to 70,261
GFF2412
GFF2413 - Imidazoleglycerol-phosphate dehydratase, at 70,430 to 71,023
GFF2413
GFF2414 - hypothetical protein, at 71,058 to 71,543
GFF2414
GFF2415 - Imidazole glycerol phosphate synthase subunit HisH, at 71,547 to 72,203
GFF2415
GFF2416 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino] imidazole-4-carboxamide isomerase, at 72,200 to 72,928
GFF2416
Position (kb) 71
72 Strain fitness (log2 ratio) -1
0
1 at 70.069 kb on - strand, within GFF2412 at 70.300 kb on - strand at 70.300 kb on - strand at 70.300 kb on - strand at 70.379 kb on - strand at 71.217 kb on + strand, within GFF2414 at 71.217 kb on + strand, within GFF2414 at 71.217 kb on + strand, within GFF2414 at 71.217 kb on + strand, within GFF2414 at 71.364 kb on + strand, within GFF2414
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 70,069 - GFF2412 0.76 +0.4 70,300 - +0.7 70,300 - -0.2 70,300 - -1.2 70,379 - +0.4 71,217 + GFF2414 0.33 -0.0 71,217 + GFF2414 0.33 -0.6 71,217 + GFF2414 0.33 -0.5 71,217 + GFF2414 0.33 +1.0 71,364 + GFF2414 0.63 +0.9
Or see this region's nucleotide sequence