Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF154 and GFF155 are separated by 222 nucleotides GFF155 and GFF156 overlap by 4 nucleotides GFF156 and GFF157 are separated by 100 nucleotides GFF157 and GFF158 are separated by 68 nucleotides
GFF154 - sugar efflux transporter, at 164,624 to 165,805
GFF154
GFF155 - hypothetical protein, at 166,028 to 166,534
GFF155
GFF156 - hypothetical protein, at 166,531 to 167,190
GFF156
GFF157 - RNA pyrophosphohydrolase, at 167,291 to 167,755
GFF157
GFF158 - hypothetical protein, at 167,824 to 168,231
GFF158
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1 at 165.561 kb on - strand, within GFF154 at 165.711 kb on + strand at 165.711 kb on + strand at 165.711 kb on + strand at 165.714 kb on + strand at 165.715 kb on - strand at 165.715 kb on - strand at 165.715 kb on - strand at 165.760 kb on + strand at 165.760 kb on + strand at 165.760 kb on + strand at 165.910 kb on - strand at 166.602 kb on - strand, within GFF156 at 166.602 kb on - strand, within GFF156 at 166.697 kb on + strand, within GFF156 at 166.811 kb on + strand, within GFF156 at 166.862 kb on + strand, within GFF156 at 166.862 kb on + strand, within GFF156 at 167.221 kb on + strand at 167.222 kb on - strand at 167.933 kb on - strand, within GFF158 at 168.087 kb on + strand, within GFF158 at 168.119 kb on - strand, within GFF158
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 165,561 - GFF154 0.79 +0.3 165,711 + +0.1 165,711 + +0.3 165,711 + +0.6 165,714 + -0.2 165,715 - -1.3 165,715 - -0.2 165,715 - -0.1 165,760 + +0.3 165,760 + -0.3 165,760 + +0.5 165,910 - -0.5 166,602 - GFF156 0.11 +1.0 166,602 - GFF156 0.11 -0.1 166,697 + GFF156 0.25 -0.2 166,811 + GFF156 0.42 -0.3 166,862 + GFF156 0.50 -0.1 166,862 + GFF156 0.50 +0.4 167,221 + -0.1 167,222 - -0.4 167,933 - GFF158 0.27 +0.1 168,087 + GFF158 0.64 +1.6 168,119 - GFF158 0.72 +1.7
Or see this region's nucleotide sequence