Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF134 and GFF135 are separated by 105 nucleotides GFF135 and GFF136 are separated by 107 nucleotides GFF136 and GFF137 are separated by 206 nucleotides GFF137 and GFF138 are separated by 87 nucleotides
GFF134 - Transcriptional regulatory protein DegU, at 146,228 to 146,902
GFF134
GFF135 - hypothetical protein, at 147,008 to 147,481
GFF135
GFF136 - Acetyl esterase, at 147,589 to 148,545
GFF136
GFF137 - hypothetical protein, at 148,752 to 149,219
GFF137
GFF138 - 2-hydroxychromene-2-carboxylate isomerase, at 149,307 to 149,951
GFF138
Position (kb) 147
148
149 Strain fitness (log2 ratio) -1
0
1 at 146.677 kb on - strand, within GFF134 at 147.087 kb on + strand, within GFF135 at 147.523 kb on + strand at 147.563 kb on + strand at 147.863 kb on - strand, within GFF136 at 147.971 kb on - strand, within GFF136 at 147.971 kb on - strand, within GFF136 at 148.190 kb on - strand, within GFF136 at 148.280 kb on - strand, within GFF136 at 148.280 kb on - strand, within GFF136 at 148.454 kb on - strand at 148.454 kb on - strand at 148.528 kb on + strand at 148.814 kb on - strand, within GFF137 at 148.963 kb on + strand, within GFF137 at 148.964 kb on - strand, within GFF137 at 148.964 kb on - strand, within GFF137 at 149.270 kb on - strand at 149.407 kb on + strand, within GFF138 at 149.408 kb on - strand, within GFF138 at 149.462 kb on - strand, within GFF138
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 146,677 - GFF134 0.67 +1.0 147,087 + GFF135 0.17 -0.4 147,523 + -0.9 147,563 + +0.1 147,863 - GFF136 0.29 -0.1 147,971 - GFF136 0.40 +0.9 147,971 - GFF136 0.40 +0.2 148,190 - GFF136 0.63 +0.4 148,280 - GFF136 0.72 -0.2 148,280 - GFF136 0.72 +0.7 148,454 - +1.5 148,454 - +1.7 148,528 + -0.6 148,814 - GFF137 0.13 -0.5 148,963 + GFF137 0.45 -0.0 148,964 - GFF137 0.45 -0.2 148,964 - GFF137 0.45 +0.8 149,270 - -0.5 149,407 + GFF138 0.16 +0.6 149,408 - GFF138 0.16 -0.3 149,462 - GFF138 0.24 +0.4
Or see this region's nucleotide sequence