Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1300 and GFF1301 are separated by 36 nucleotides GFF1301 and GFF1302 are separated by 54 nucleotides GFF1302 and GFF1303 overlap by 14 nucleotides GFF1303 and GFF1304 overlap by 4 nucleotides
GFF1300 - Protein YffB, at 1,406,351 to 1,406,698
GFF1300
GFF1301 - hypothetical protein, at 1,406,735 to 1,407,208
GFF1301
GFF1302 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 1,407,263 to 1,407,970
GFF1302
GFF1303 - Lipopolysaccharide export system ATP-binding protein LptB, at 1,407,957 to 1,408,709
GFF1303
GFF1304 - hypothetical protein, at 1,408,706 to 1,409,713
GFF1304
Position (kb)
1407
1408 Strain fitness (log2 ratio)
-2
-1
0
1 at 1406.366 kb on + strand at 1406.366 kb on + strand at 1406.366 kb on + strand at 1406.366 kb on + strand at 1406.367 kb on - strand at 1406.433 kb on - strand, within GFF1300 at 1406.433 kb on - strand, within GFF1300 at 1406.447 kb on + strand, within GFF1300 at 1406.448 kb on - strand, within GFF1300 at 1406.448 kb on - strand, within GFF1300 at 1406.678 kb on + strand at 1406.678 kb on + strand at 1406.890 kb on - strand, within GFF1301 at 1406.890 kb on - strand, within GFF1301 at 1407.079 kb on - strand, within GFF1301 at 1407.079 kb on - strand, within GFF1301 at 1407.079 kb on - strand, within GFF1301 at 1407.276 kb on + strand at 1407.276 kb on + strand at 1407.276 kb on + strand at 1407.276 kb on + strand at 1407.277 kb on - strand at 1407.508 kb on + strand, within GFF1302 at 1407.617 kb on + strand, within GFF1302 at 1407.726 kb on + strand, within GFF1302 at 1407.727 kb on - strand, within GFF1302 at 1407.727 kb on - strand, within GFF1302 at 1407.964 kb on + strand at 1407.964 kb on + strand at 1407.965 kb on - strand at 1407.985 kb on + strand at 1408.379 kb on - strand, within GFF1303 at 1408.456 kb on + strand, within GFF1303
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 1,406,366 + +0.2 1,406,366 + +0.5 1,406,366 + +0.6 1,406,366 + -0.1 1,406,367 - -0.4 1,406,433 - GFF1300 0.24 -0.3 1,406,433 - GFF1300 0.24 -1.4 1,406,447 + GFF1300 0.28 +0.6 1,406,448 - GFF1300 0.28 -1.3 1,406,448 - GFF1300 0.28 +0.3 1,406,678 + +1.0 1,406,678 + -0.7 1,406,890 - GFF1301 0.33 -0.8 1,406,890 - GFF1301 0.33 +0.2 1,407,079 - GFF1301 0.73 -1.8 1,407,079 - GFF1301 0.73 -0.2 1,407,079 - GFF1301 0.73 +1.5 1,407,276 + +1.0 1,407,276 + +0.2 1,407,276 + +0.6 1,407,276 + +1.0 1,407,277 - -0.5 1,407,508 + GFF1302 0.35 -0.7 1,407,617 + GFF1302 0.50 +0.6 1,407,726 + GFF1302 0.65 -0.6 1,407,727 - GFF1302 0.66 -0.7 1,407,727 - GFF1302 0.66 -0.9 1,407,964 + +0.6 1,407,964 + +0.3 1,407,965 - -0.3 1,407,985 + +0.1 1,408,379 - GFF1303 0.56 -1.1 1,408,456 + GFF1303 0.66 +0.2
Or see this region's nucleotide sequence