Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF108 and GFF109 are separated by 94 nucleotides GFF109 and GFF110 are separated by 5 nucleotides GFF110 and GFF111 are separated by 89 nucleotides GFF111 and GFF112 are separated by 123 nucleotides
GFF108 - hypothetical protein, at 118,492 to 120,372
GFF108
GFF109 - tRNA-specific adenosine deaminase, at 120,467 to 120,895
GFF109
GFF110 - Fluoroacetate dehalogenase, at 120,901 to 121,812
GFF110
GFF111 - hypothetical protein, at 121,902 to 122,189
GFF111
GFF112 - Penicillin-insensitive murein endopeptidase, at 122,313 to 123,194
GFF112
Position (kb) 120
121
122 Strain fitness (log2 ratio) -2
-1
0
1 at 120.140 kb on - strand, within GFF108 at 120.140 kb on - strand, within GFF108 at 120.894 kb on - strand at 120.903 kb on - strand at 120.903 kb on - strand at 121.352 kb on + strand, within GFF110 at 121.352 kb on + strand, within GFF110 at 121.398 kb on - strand, within GFF110 at 122.032 kb on + strand, within GFF111 at 122.032 kb on + strand, within GFF111 at 122.058 kb on - strand, within GFF111
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 120,140 - GFF108 0.88 -1.3 120,140 - GFF108 0.88 -0.7 120,894 - -1.4 120,903 - -1.3 120,903 - -1.4 121,352 + GFF110 0.49 -1.8 121,352 + GFF110 0.49 -0.4 121,398 - GFF110 0.54 -1.0 122,032 + GFF111 0.45 -1.0 122,032 + GFF111 0.45 +0.2 122,058 - GFF111 0.54 -0.1
Or see this region's nucleotide sequence