Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1059 and GFF1060 are separated by 31 nucleotides GFF1060 and GFF1061 are separated by 170 nucleotides GFF1061 and GFF1062 are separated by 100 nucleotides GFF1062 and GFF1063 are separated by 59 nucleotides
GFF1059 - Phosphoribosylformylglycinamidine synthase subunit PurL, at 1,138,688 to 1,140,904
GFF1059
GFF1060 - putative protein RP812, at 1,140,936 to 1,141,172
GFF1060
GFF1061 - Glutaredoxin 4, at 1,141,343 to 1,141,681
GFF1061
GFF1062 - hypothetical protein, at 1,141,782 to 1,142,039
GFF1062
GFF1063 - Bifunctional protein FolD protein, at 1,142,099 to 1,142,974
GFF1063
Position (kb) 1140
1141
1142 Strain fitness (log2 ratio) -2
-1
0
1 at 1141.032 kb on + strand, within GFF1060 at 1141.098 kb on + strand, within GFF1060 at 1141.098 kb on + strand, within GFF1060 at 1141.098 kb on + strand, within GFF1060 at 1141.099 kb on - strand, within GFF1060 at 1141.171 kb on - strand at 1141.563 kb on - strand, within GFF1061 at 1142.037 kb on + strand at 1142.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 1,141,032 + GFF1060 0.41 +0.4 1,141,098 + GFF1060 0.68 -0.9 1,141,098 + GFF1060 0.68 -1.8 1,141,098 + GFF1060 0.68 -1.4 1,141,099 - GFF1060 0.69 +1.2 1,141,171 - +0.8 1,141,563 - GFF1061 0.65 +0.6 1,142,037 + +1.2 1,142,101 - -0.4
Or see this region's nucleotide sequence