Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3766 and GFF3767 are separated by 85 nucleotides GFF3767 and GFF3768 are separated by 36 nucleotides GFF3768 and GFF3769 are separated by 16 nucleotides
GFF3766 - O-acetyl-ADP-ribose deacetylase, at 113,241 to 113,810
GFF3766
GFF3767 - hypothetical protein, at 113,896 to 114,423
GFF3767
GFF3768 - hypothetical protein, at 114,460 to 115,500
GFF3768
GFF3769 - D-beta-hydroxybutyrate dehydrogenase, at 115,517 to 116,299
GFF3769
Position (kb) 114
115
116 Strain fitness (log2 ratio) -2
-1
0
1 at 113.845 kb on + strand at 113.845 kb on + strand at 113.857 kb on + strand at 113.884 kb on + strand at 114.777 kb on + strand, within GFF3768 at 114.872 kb on + strand, within GFF3768 at 114.902 kb on + strand, within GFF3768 at 114.903 kb on - strand, within GFF3768 at 115.026 kb on + strand, within GFF3768 at 115.027 kb on - strand, within GFF3768 at 115.181 kb on + strand, within GFF3768 at 115.740 kb on + strand, within GFF3769 at 115.740 kb on + strand, within GFF3769 at 115.836 kb on + strand, within GFF3769 at 115.836 kb on + strand, within GFF3769 at 115.837 kb on - strand, within GFF3769 at 115.837 kb on - strand, within GFF3769 at 116.044 kb on + strand, within GFF3769 at 116.045 kb on - strand, within GFF3769 at 116.173 kb on - strand, within GFF3769 at 116.241 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 113,845 + -1.5 113,845 + -0.0 113,857 + -1.3 113,884 + -0.0 114,777 + GFF3768 0.30 -0.4 114,872 + GFF3768 0.40 +0.3 114,902 + GFF3768 0.42 +0.3 114,903 - GFF3768 0.43 +1.4 115,026 + GFF3768 0.54 -0.2 115,027 - GFF3768 0.54 -0.4 115,181 + GFF3768 0.69 +0.8 115,740 + GFF3769 0.28 +0.9 115,740 + GFF3769 0.28 +0.0 115,836 + GFF3769 0.41 -0.3 115,836 + GFF3769 0.41 +0.5 115,837 - GFF3769 0.41 -0.9 115,837 - GFF3769 0.41 -0.0 116,044 + GFF3769 0.67 -0.7 116,045 - GFF3769 0.67 +0.3 116,173 - GFF3769 0.84 +0.4 116,241 + -2.7
Or see this region's nucleotide sequence