Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1519 and GFF1520 are separated by 119 nucleotides GFF1520 and GFF1521 are separated by 97 nucleotides
GFF1519 - Betaine aldehyde dehydrogenase, at 158,404 to 159,861
GFF1519
GFF1520 - Imidazolonepropionase, at 159,981 to 161,237
GFF1520
GFF1521 - putative nicotinate-nucleotide pyrophosphorylase [carboxylating], at 161,335 to 162,225
GFF1521
Position (kb) 159
160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2 at 159.234 kb on - strand, within GFF1519 at 159.423 kb on - strand, within GFF1519 at 159.634 kb on + strand, within GFF1519 at 159.635 kb on - strand, within GFF1519 at 159.635 kb on - strand, within GFF1519 at 159.807 kb on - strand at 159.860 kb on - strand at 159.980 kb on + strand at 159.981 kb on - strand at 160.070 kb on + strand at 160.186 kb on - strand, within GFF1520 at 160.186 kb on - strand, within GFF1520 at 160.186 kb on - strand, within GFF1520 at 160.230 kb on + strand, within GFF1520 at 160.230 kb on + strand, within GFF1520 at 160.231 kb on - strand, within GFF1520 at 160.231 kb on - strand, within GFF1520 at 160.411 kb on - strand, within GFF1520 at 160.881 kb on + strand, within GFF1520 at 161.288 kb on + strand at 161.288 kb on + strand at 161.295 kb on + strand at 161.296 kb on - strand at 161.333 kb on + strand at 161.333 kb on + strand at 161.333 kb on + strand at 161.333 kb on + strand at 161.334 kb on - strand at 161.353 kb on - strand at 161.394 kb on - strand at 161.455 kb on - strand, within GFF1521 at 161.455 kb on - strand, within GFF1521 at 161.455 kb on - strand, within GFF1521 at 161.458 kb on + strand, within GFF1521 at 161.458 kb on + strand, within GFF1521 at 161.458 kb on + strand, within GFF1521 at 161.687 kb on - strand, within GFF1521 at 161.767 kb on + strand, within GFF1521 at 161.767 kb on + strand, within GFF1521 at 161.767 kb on + strand, within GFF1521 at 161.767 kb on + strand, within GFF1521 at 161.768 kb on - strand, within GFF1521 at 162.080 kb on + strand, within GFF1521 at 162.080 kb on + strand, within GFF1521
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 159,234 - GFF1519 0.57 +0.2 159,423 - GFF1519 0.70 -0.1 159,634 + GFF1519 0.84 +0.3 159,635 - GFF1519 0.84 -0.2 159,635 - GFF1519 0.84 -1.1 159,807 - -0.0 159,860 - +0.9 159,980 + +1.2 159,981 - -0.0 160,070 + +0.6 160,186 - GFF1520 0.16 -0.8 160,186 - GFF1520 0.16 -0.0 160,186 - GFF1520 0.16 +0.3 160,230 + GFF1520 0.20 -0.0 160,230 + GFF1520 0.20 +0.1 160,231 - GFF1520 0.20 +1.9 160,231 - GFF1520 0.20 -0.1 160,411 - GFF1520 0.34 -0.7 160,881 + GFF1520 0.72 -0.6 161,288 + -0.1 161,288 + -0.6 161,295 + -1.9 161,296 - +0.5 161,333 + -0.5 161,333 + +2.1 161,333 + +0.1 161,333 + +0.2 161,334 - +0.3 161,353 - +0.1 161,394 - +0.4 161,455 - GFF1521 0.13 -0.7 161,455 - GFF1521 0.13 -0.7 161,455 - GFF1521 0.13 +0.4 161,458 + GFF1521 0.14 -0.1 161,458 + GFF1521 0.14 -0.3 161,458 + GFF1521 0.14 -0.5 161,687 - GFF1521 0.40 -0.2 161,767 + GFF1521 0.48 +1.4 161,767 + GFF1521 0.48 +1.1 161,767 + GFF1521 0.48 -0.1 161,767 + GFF1521 0.48 -1.2 161,768 - GFF1521 0.49 -0.2 162,080 + GFF1521 0.84 +0.8 162,080 + GFF1521 0.84 -0.6
Or see this region's nucleotide sequence