Strain Fitness in Xanthobacter sp. DMC5 around GFF85

Experiment: Methanol and casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF84 and GFF85 are separated by 42 nucleotidesGFF85 and GFF86 are separated by 324 nucleotidesGFF86 and GFF87 are separated by 4 nucleotides GFF84 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 93,073 to 93,894 GFF84 GFF85 - 5-formyltetrahydrofolate cyclo-ligase, at 93,937 to 94,464 GFF85 GFF86 - hypothetical protein, at 94,789 to 95,172 GFF86 GFF87 - hypothetical protein, at 95,177 to 95,482 GFF87 Position (kb) 93 94 95Strain fitness (log2 ratio) -1 0 1at 92.991 kb on + strandat 92.992 kb on - strandat 92.992 kb on - strandat 93.312 kb on - strand, within GFF84at 93.312 kb on - strand, within GFF84at 93.537 kb on + strand, within GFF84at 94.047 kb on - strand, within GFF85at 94.047 kb on - strand, within GFF85at 94.100 kb on + strand, within GFF85at 94.101 kb on - strand, within GFF85at 94.464 kb on + strandat 94.623 kb on - strandat 94.629 kb on + strandat 94.642 kb on + strandat 94.642 kb on + strandat 94.642 kb on + strandat 94.643 kb on - strandat 94.745 kb on + strandat 94.746 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol and casaminos (C)
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92,991 + +0.5
92,992 - -0.7
92,992 - -0.6
93,312 - GFF84 0.29 -0.2
93,312 - GFF84 0.29 -1.5
93,537 + GFF84 0.56 -0.9
94,047 - GFF85 0.21 -1.6
94,047 - GFF85 0.21 +0.1
94,100 + GFF85 0.31 -0.4
94,101 - GFF85 0.31 +0.4
94,464 + +0.1
94,623 - +0.1
94,629 + +0.8
94,642 + +0.6
94,642 + -0.1
94,642 + -0.1
94,643 - +0.8
94,745 + -0.3
94,746 - -0.8

Or see this region's nucleotide sequence