Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF829 and GFF830 are separated by 119 nucleotides GFF830 and GFF831 overlap by 4 nucleotides GFF831 and GFF832 are separated by 174 nucleotides
GFF829 - Outer membrane protein assembly factor BamE, at 892,557 to 893,093
GFF829
GFF830 - hypothetical protein, at 893,213 to 893,776
GFF830
GFF831 - hypothetical protein, at 893,773 to 894,279
GFF831
GFF832 - Phosphate acyltransferase, at 894,454 to 895,524
GFF832
Position (kb) 893
894
895 Strain fitness (log2 ratio) -1
0
1 at 893.274 kb on - strand, within GFF830 at 893.335 kb on + strand, within GFF830 at 893.411 kb on - strand, within GFF830 at 893.537 kb on + strand, within GFF830 at 893.561 kb on - strand, within GFF830 at 893.614 kb on + strand, within GFF830 at 893.615 kb on - strand, within GFF830 at 893.664 kb on - strand, within GFF830 at 893.789 kb on - strand at 893.789 kb on - strand at 893.789 kb on - strand at 894.067 kb on + strand, within GFF831 at 894.067 kb on + strand, within GFF831 at 894.068 kb on - strand, within GFF831 at 894.068 kb on - strand, within GFF831 at 894.068 kb on - strand, within GFF831 at 894.068 kb on - strand, within GFF831 at 894.068 kb on - strand, within GFF831 at 894.068 kb on - strand, within GFF831 at 894.070 kb on - strand, within GFF831 at 894.274 kb on + strand at 894.277 kb on + strand at 894.278 kb on - strand at 894.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 893,274 - GFF830 0.11 +0.7 893,335 + GFF830 0.22 -1.3 893,411 - GFF830 0.35 +0.4 893,537 + GFF830 0.57 -0.9 893,561 - GFF830 0.62 -0.7 893,614 + GFF830 0.71 -0.5 893,615 - GFF830 0.71 -0.4 893,664 - GFF830 0.80 -0.1 893,789 - -0.0 893,789 - -0.2 893,789 - -1.0 894,067 + GFF831 0.58 -0.4 894,067 + GFF831 0.58 +0.1 894,068 - GFF831 0.58 -0.2 894,068 - GFF831 0.58 +0.4 894,068 - GFF831 0.58 +0.3 894,068 - GFF831 0.58 +0.3 894,068 - GFF831 0.58 -0.5 894,068 - GFF831 0.58 -0.1 894,070 - GFF831 0.59 +0.5 894,274 + -0.9 894,277 + +0.1 894,278 - +0.3 894,326 + +0.7
Or see this region's nucleotide sequence