Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF653 and GFF654 are separated by 18 nucleotides GFF654 and GFF655 are separated by 293 nucleotides GFF655 and GFF656 overlap by 4 nucleotides GFF656 and GFF657 are separated by 305 nucleotides
GFF653 - Ferredoxin--NADP reductase, at 701,509 to 702,282
GFF653
GFF654 - Ferredoxin-2, at 702,301 to 702,633
GFF654
GFF655 - Nicotinate dehydrogenase subunit B, at 702,927 to 706,475
GFF655
GFF656 - Nicotinate dehydrogenase subunit A, at 706,472 to 706,960
GFF656
GFF657 - 6-hydroxynicotinate reductase, at 707,266 to 708,747
GFF657
Position (kb) 702
703
704
705
706
707 Strain fitness (log2 ratio) -1
0
1
2 at 701.951 kb on + strand, within GFF653 at 701.951 kb on + strand, within GFF653 at 701.968 kb on + strand, within GFF653 at 702.126 kb on - strand, within GFF653 at 702.303 kb on - strand at 702.417 kb on - strand, within GFF654 at 702.555 kb on - strand, within GFF654 at 702.593 kb on + strand, within GFF654 at 702.594 kb on - strand, within GFF654 at 702.626 kb on + strand at 702.626 kb on + strand at 702.627 kb on - strand at 702.627 kb on - strand at 702.989 kb on + strand at 703.348 kb on + strand, within GFF655 at 703.348 kb on + strand, within GFF655 at 703.349 kb on - strand, within GFF655 at 703.804 kb on + strand, within GFF655 at 703.804 kb on + strand, within GFF655 at 703.804 kb on + strand, within GFF655 at 703.849 kb on + strand, within GFF655 at 703.849 kb on + strand, within GFF655 at 703.922 kb on - strand, within GFF655 at 703.922 kb on - strand, within GFF655 at 704.133 kb on + strand, within GFF655 at 704.134 kb on - strand, within GFF655 at 704.452 kb on + strand, within GFF655 at 704.701 kb on + strand, within GFF655 at 704.702 kb on - strand, within GFF655 at 704.702 kb on - strand, within GFF655 at 704.702 kb on - strand, within GFF655 at 704.711 kb on - strand, within GFF655 at 704.893 kb on + strand, within GFF655 at 704.894 kb on - strand, within GFF655 at 704.995 kb on + strand, within GFF655 at 704.996 kb on - strand, within GFF655 at 705.002 kb on - strand, within GFF655 at 705.035 kb on - strand, within GFF655 at 705.035 kb on - strand, within GFF655 at 705.298 kb on + strand, within GFF655 at 705.299 kb on - strand, within GFF655 at 705.485 kb on - strand, within GFF655 at 705.803 kb on - strand, within GFF655 at 705.859 kb on + strand, within GFF655 at 705.860 kb on - strand, within GFF655 at 705.860 kb on - strand, within GFF655 at 705.890 kb on + strand, within GFF655 at 706.001 kb on - strand, within GFF655 at 706.656 kb on + strand, within GFF656 at 706.884 kb on + strand, within GFF656 at 706.885 kb on - strand, within GFF656 at 706.885 kb on - strand, within GFF656 at 707.218 kb on + strand at 707.219 kb on - strand at 707.341 kb on + strand at 707.341 kb on + strand at 707.341 kb on + strand at 707.342 kb on - strand at 707.342 kb on - strand at 707.377 kb on + strand at 707.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 701,951 + GFF653 0.57 +0.5 701,951 + GFF653 0.57 +0.0 701,968 + GFF653 0.59 +0.3 702,126 - GFF653 0.80 +0.3 702,303 - +1.0 702,417 - GFF654 0.35 -0.5 702,555 - GFF654 0.76 -0.0 702,593 + GFF654 0.88 -0.5 702,594 - GFF654 0.88 +0.4 702,626 + -0.0 702,626 + +0.3 702,627 - -0.4 702,627 - -0.3 702,989 + +0.3 703,348 + GFF655 0.12 -0.2 703,348 + GFF655 0.12 +0.5 703,349 - GFF655 0.12 -0.3 703,804 + GFF655 0.25 -0.3 703,804 + GFF655 0.25 -1.4 703,804 + GFF655 0.25 +0.1 703,849 + GFF655 0.26 -0.7 703,849 + GFF655 0.26 +1.8 703,922 - GFF655 0.28 -0.5 703,922 - GFF655 0.28 +1.1 704,133 + GFF655 0.34 -0.6 704,134 - GFF655 0.34 -1.1 704,452 + GFF655 0.43 +0.5 704,701 + GFF655 0.50 +0.5 704,702 - GFF655 0.50 -0.6 704,702 - GFF655 0.50 -0.2 704,702 - GFF655 0.50 +1.6 704,711 - GFF655 0.50 -1.3 704,893 + GFF655 0.55 -0.7 704,894 - GFF655 0.55 -0.0 704,995 + GFF655 0.58 -0.0 704,996 - GFF655 0.58 -0.0 705,002 - GFF655 0.58 +0.4 705,035 - GFF655 0.59 +0.7 705,035 - GFF655 0.59 -0.3 705,298 + GFF655 0.67 +1.5 705,299 - GFF655 0.67 -0.1 705,485 - GFF655 0.72 +0.2 705,803 - GFF655 0.81 -0.3 705,859 + GFF655 0.83 +0.3 705,860 - GFF655 0.83 +0.2 705,860 - GFF655 0.83 +0.6 705,890 + GFF655 0.83 +0.4 706,001 - GFF655 0.87 -0.6 706,656 + GFF656 0.38 +0.5 706,884 + GFF656 0.84 -0.6 706,885 - GFF656 0.84 +0.4 706,885 - GFF656 0.84 +2.1 707,218 + +1.1 707,219 - -0.2 707,341 + -0.8 707,341 + -0.3 707,341 + -0.4 707,342 - -0.0 707,342 - -0.8 707,377 + +0.2 707,378 - -0.0
Or see this region's nucleotide sequence