Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4705 and GFF4706 overlap by 8 nucleotides GFF4706 and GFF4707 are separated by 9 nucleotides GFF4707 and GFF4708 are separated by 80 nucleotides
GFF4705 - 6'''-hydroxyparomomycin C oxidase, at 116,657 to 118,303
GFF4705
GFF4706 - hypothetical protein, at 118,296 to 118,643
GFF4706
GFF4707 - Galactose-proton symporter, at 118,653 to 120,017
GFF4707
GFF4708 - 8-amino-3,8-dideoxy-alpha-D-manno-octulosonate transaminase, at 120,098 to 121,420
GFF4708
Position (kb) 118
119
120
121 Strain fitness (log2 ratio) -2
-1
0
1 at 117.753 kb on + strand, within GFF4705 at 117.825 kb on + strand, within GFF4705 at 117.825 kb on + strand, within GFF4705 at 117.826 kb on - strand, within GFF4705 at 117.826 kb on - strand, within GFF4705 at 117.826 kb on - strand, within GFF4705 at 117.898 kb on - strand, within GFF4705 at 117.898 kb on - strand, within GFF4705 at 118.298 kb on - strand at 118.400 kb on - strand, within GFF4706 at 118.400 kb on - strand, within GFF4706 at 118.570 kb on + strand, within GFF4706 at 118.913 kb on + strand, within GFF4707 at 118.981 kb on + strand, within GFF4707 at 118.982 kb on - strand, within GFF4707 at 118.982 kb on - strand, within GFF4707 at 118.982 kb on - strand, within GFF4707 at 118.982 kb on - strand, within GFF4707 at 119.230 kb on + strand, within GFF4707 at 119.233 kb on + strand, within GFF4707 at 119.234 kb on - strand, within GFF4707 at 119.234 kb on - strand, within GFF4707 at 119.380 kb on + strand, within GFF4707 at 119.585 kb on - strand, within GFF4707 at 119.620 kb on + strand, within GFF4707 at 119.671 kb on + strand, within GFF4707 at 119.671 kb on + strand, within GFF4707 at 119.671 kb on + strand, within GFF4707 at 119.671 kb on + strand, within GFF4707 at 119.671 kb on + strand, within GFF4707 at 119.672 kb on - strand, within GFF4707 at 120.037 kb on + strand at 120.238 kb on - strand, within GFF4708 at 120.243 kb on + strand, within GFF4708 at 120.243 kb on + strand, within GFF4708 at 120.244 kb on - strand, within GFF4708 at 120.333 kb on + strand, within GFF4708 at 120.333 kb on + strand, within GFF4708 at 120.334 kb on - strand, within GFF4708 at 120.571 kb on + strand, within GFF4708
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 117,753 + GFF4705 0.67 -0.5 117,825 + GFF4705 0.71 -0.4 117,825 + GFF4705 0.71 -0.2 117,826 - GFF4705 0.71 +0.4 117,826 - GFF4705 0.71 -0.0 117,826 - GFF4705 0.71 -2.6 117,898 - GFF4705 0.75 -0.0 117,898 - GFF4705 0.75 -0.1 118,298 - -0.4 118,400 - GFF4706 0.30 +0.1 118,400 - GFF4706 0.30 -0.3 118,570 + GFF4706 0.79 +1.2 118,913 + GFF4707 0.19 -0.2 118,981 + GFF4707 0.24 +0.3 118,982 - GFF4707 0.24 +0.1 118,982 - GFF4707 0.24 +0.4 118,982 - GFF4707 0.24 +0.8 118,982 - GFF4707 0.24 -0.1 119,230 + GFF4707 0.42 -0.4 119,233 + GFF4707 0.42 +0.2 119,234 - GFF4707 0.43 -0.3 119,234 - GFF4707 0.43 -1.3 119,380 + GFF4707 0.53 +0.4 119,585 - GFF4707 0.68 -0.3 119,620 + GFF4707 0.71 +0.5 119,671 + GFF4707 0.75 -0.6 119,671 + GFF4707 0.75 +0.5 119,671 + GFF4707 0.75 +0.5 119,671 + GFF4707 0.75 -0.1 119,671 + GFF4707 0.75 +0.2 119,672 - GFF4707 0.75 -1.6 120,037 + -1.0 120,238 - GFF4708 0.11 -0.6 120,243 + GFF4708 0.11 +0.2 120,243 + GFF4708 0.11 -0.2 120,244 - GFF4708 0.11 +0.6 120,333 + GFF4708 0.18 +0.2 120,333 + GFF4708 0.18 -0.1 120,334 - GFF4708 0.18 -0.7 120,571 + GFF4708 0.36 +0.6
Or see this region's nucleotide sequence