Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4649 and GFF4650 are separated by 18 nucleotides GFF4650 and GFF4651 are separated by 132 nucleotides GFF4651 and GFF4652 are separated by 326 nucleotides GFF4652 and GFF4653 are separated by 58 nucleotides
GFF4649 - NADP/NAD-dependent aldehyde dehydrogenase PuuC, at 57,783 to 59,264
GFF4649
GFF4650 - Thiopurine S-methyltransferase, at 59,283 to 59,921
GFF4650
GFF4651 - Putrescine--pyruvate aminotransferase, at 60,054 to 61,487
GFF4651
GFF4652 - hypothetical protein, at 61,814 to 62,215
GFF4652
GFF4653 - putative GST-like protein YibF, at 62,274 to 62,873
GFF4653
Position (kb) 60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 59.130 kb on + strand at 59.130 kb on + strand at 59.229 kb on + strand at 59.378 kb on - strand, within GFF4650 at 59.452 kb on + strand, within GFF4650 at 59.513 kb on - strand, within GFF4650 at 59.561 kb on - strand, within GFF4650 at 59.761 kb on + strand, within GFF4650 at 59.761 kb on + strand, within GFF4650 at 59.849 kb on - strand, within GFF4650 at 59.849 kb on - strand, within GFF4650 at 59.961 kb on + strand at 59.962 kb on - strand at 60.189 kb on + strand at 60.189 kb on + strand at 60.189 kb on + strand at 60.190 kb on - strand at 60.190 kb on - strand at 60.190 kb on - strand at 60.190 kb on - strand at 60.313 kb on - strand, within GFF4651 at 60.315 kb on + strand, within GFF4651 at 60.315 kb on + strand, within GFF4651 at 60.316 kb on - strand, within GFF4651 at 60.316 kb on - strand, within GFF4651 at 60.438 kb on - strand, within GFF4651 at 60.498 kb on + strand, within GFF4651 at 60.621 kb on + strand, within GFF4651 at 60.622 kb on - strand, within GFF4651 at 60.783 kb on + strand, within GFF4651 at 60.784 kb on - strand, within GFF4651 at 60.784 kb on - strand, within GFF4651 at 60.942 kb on + strand, within GFF4651 at 60.943 kb on - strand, within GFF4651 at 61.023 kb on + strand, within GFF4651 at 61.024 kb on - strand, within GFF4651 at 61.024 kb on - strand, within GFF4651 at 61.024 kb on - strand, within GFF4651 at 61.030 kb on - strand, within GFF4651 at 61.129 kb on - strand, within GFF4651 at 61.622 kb on + strand at 61.623 kb on - strand at 61.623 kb on - strand at 61.739 kb on + strand at 61.739 kb on + strand at 61.810 kb on + strand at 61.810 kb on + strand at 61.814 kb on - strand at 62.030 kb on + strand, within GFF4652 at 62.030 kb on + strand, within GFF4652 at 62.030 kb on + strand, within GFF4652 at 62.031 kb on - strand, within GFF4652 at 62.115 kb on - strand, within GFF4652 at 62.115 kb on - strand, within GFF4652 at 62.115 kb on - strand, within GFF4652 at 62.139 kb on - strand, within GFF4652 at 62.144 kb on + strand, within GFF4652 at 62.201 kb on + strand at 62.201 kb on + strand at 62.202 kb on - strand at 62.202 kb on - strand at 62.440 kb on + strand, within GFF4653
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 59,130 + -0.4 59,130 + -0.9 59,229 + -0.0 59,378 - GFF4650 0.15 -0.6 59,452 + GFF4650 0.26 +0.2 59,513 - GFF4650 0.36 -0.4 59,561 - GFF4650 0.44 -0.3 59,761 + GFF4650 0.75 +0.5 59,761 + GFF4650 0.75 +0.7 59,849 - GFF4650 0.89 +0.1 59,849 - GFF4650 0.89 -0.4 59,961 + +0.2 59,962 - +0.4 60,189 + -0.9 60,189 + +0.5 60,189 + -0.3 60,190 - -1.2 60,190 - -1.8 60,190 - -2.8 60,190 - -0.9 60,313 - GFF4651 0.18 -1.0 60,315 + GFF4651 0.18 -0.9 60,315 + GFF4651 0.18 -1.1 60,316 - GFF4651 0.18 -1.6 60,316 - GFF4651 0.18 -0.4 60,438 - GFF4651 0.27 -1.8 60,498 + GFF4651 0.31 -0.3 60,621 + GFF4651 0.40 -1.4 60,622 - GFF4651 0.40 +0.5 60,783 + GFF4651 0.51 -0.7 60,784 - GFF4651 0.51 -0.5 60,784 - GFF4651 0.51 +0.2 60,942 + GFF4651 0.62 +0.5 60,943 - GFF4651 0.62 -0.3 61,023 + GFF4651 0.68 -0.8 61,024 - GFF4651 0.68 +0.3 61,024 - GFF4651 0.68 -0.3 61,024 - GFF4651 0.68 +0.6 61,030 - GFF4651 0.68 -2.1 61,129 - GFF4651 0.75 +0.8 61,622 + +0.6 61,623 - -0.5 61,623 - -0.1 61,739 + -0.0 61,739 + +0.3 61,810 + -0.4 61,810 + +0.1 61,814 - +0.1 62,030 + GFF4652 0.54 -1.0 62,030 + GFF4652 0.54 +0.5 62,030 + GFF4652 0.54 -0.4 62,031 - GFF4652 0.54 -0.8 62,115 - GFF4652 0.75 +0.8 62,115 - GFF4652 0.75 +0.2 62,115 - GFF4652 0.75 +1.5 62,139 - GFF4652 0.81 -0.1 62,144 + GFF4652 0.82 -0.3 62,201 + -0.1 62,201 + -0.6 62,202 - -0.0 62,202 - -0.5 62,440 + GFF4653 0.28 +0.3
Or see this region's nucleotide sequence