Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4647 and GFF4648 are separated by 28 nucleotides GFF4648 and GFF4649 are separated by 82 nucleotides GFF4649 and GFF4650 are separated by 18 nucleotides GFF4650 and GFF4651 are separated by 132 nucleotides
GFF4647 - hypothetical protein, at 56,155 to 57,033
GFF4647
GFF4648 - hypothetical protein, at 57,062 to 57,700
GFF4648
GFF4649 - NADP/NAD-dependent aldehyde dehydrogenase PuuC, at 57,783 to 59,264
GFF4649
GFF4650 - Thiopurine S-methyltransferase, at 59,283 to 59,921
GFF4650
GFF4651 - Putrescine--pyruvate aminotransferase, at 60,054 to 61,487
GFF4651
Position (kb) 57
58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 56.939 kb on - strand, within GFF4647 at 57.087 kb on + strand at 57.087 kb on + strand at 57.097 kb on + strand at 57.200 kb on + strand, within GFF4648 at 57.200 kb on + strand, within GFF4648 at 57.201 kb on - strand, within GFF4648 at 57.201 kb on - strand, within GFF4648 at 57.240 kb on + strand, within GFF4648 at 57.240 kb on + strand, within GFF4648 at 57.241 kb on - strand, within GFF4648 at 57.248 kb on + strand, within GFF4648 at 57.249 kb on - strand, within GFF4648 at 57.398 kb on + strand, within GFF4648 at 57.423 kb on - strand, within GFF4648 at 57.423 kb on - strand, within GFF4648 at 57.423 kb on - strand, within GFF4648 at 57.423 kb on - strand, within GFF4648 at 57.674 kb on + strand at 57.674 kb on + strand at 57.674 kb on + strand at 57.675 kb on - strand at 57.675 kb on - strand at 57.675 kb on - strand at 57.675 kb on - strand at 58.158 kb on + strand, within GFF4649 at 58.158 kb on + strand, within GFF4649 at 58.159 kb on - strand, within GFF4649 at 58.161 kb on + strand, within GFF4649 at 58.185 kb on + strand, within GFF4649 at 58.185 kb on + strand, within GFF4649 at 58.186 kb on - strand, within GFF4649 at 58.522 kb on - strand, within GFF4649 at 58.654 kb on - strand, within GFF4649 at 58.827 kb on - strand, within GFF4649 at 58.827 kb on - strand, within GFF4649 at 59.049 kb on + strand, within GFF4649 at 59.049 kb on + strand, within GFF4649 at 59.049 kb on + strand, within GFF4649 at 59.130 kb on + strand at 59.130 kb on + strand at 59.229 kb on + strand at 59.378 kb on - strand, within GFF4650 at 59.452 kb on + strand, within GFF4650 at 59.513 kb on - strand, within GFF4650 at 59.561 kb on - strand, within GFF4650 at 59.761 kb on + strand, within GFF4650 at 59.761 kb on + strand, within GFF4650 at 59.849 kb on - strand, within GFF4650 at 59.849 kb on - strand, within GFF4650 at 59.961 kb on + strand at 59.962 kb on - strand at 60.189 kb on + strand at 60.189 kb on + strand at 60.189 kb on + strand at 60.190 kb on - strand at 60.190 kb on - strand at 60.190 kb on - strand at 60.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 56,939 - GFF4647 0.89 -0.3 57,087 + +0.2 57,087 + +0.8 57,097 + +0.3 57,200 + GFF4648 0.22 +0.1 57,200 + GFF4648 0.22 -0.5 57,201 - GFF4648 0.22 -0.3 57,201 - GFF4648 0.22 +0.1 57,240 + GFF4648 0.28 +0.3 57,240 + GFF4648 0.28 -0.5 57,241 - GFF4648 0.28 +0.5 57,248 + GFF4648 0.29 -0.0 57,249 - GFF4648 0.29 +0.1 57,398 + GFF4648 0.53 +0.6 57,423 - GFF4648 0.56 +0.6 57,423 - GFF4648 0.56 -0.9 57,423 - GFF4648 0.56 -0.4 57,423 - GFF4648 0.56 +0.4 57,674 + +0.0 57,674 + -1.1 57,674 + +1.1 57,675 - -0.1 57,675 - -1.0 57,675 - +1.2 57,675 - -0.2 58,158 + GFF4649 0.25 -1.1 58,158 + GFF4649 0.25 -1.2 58,159 - GFF4649 0.25 -0.4 58,161 + GFF4649 0.26 -0.2 58,185 + GFF4649 0.27 -0.6 58,185 + GFF4649 0.27 +0.9 58,186 - GFF4649 0.27 -0.1 58,522 - GFF4649 0.50 -0.3 58,654 - GFF4649 0.59 -0.4 58,827 - GFF4649 0.70 -0.1 58,827 - GFF4649 0.70 -0.2 59,049 + GFF4649 0.85 +1.5 59,049 + GFF4649 0.85 +0.1 59,049 + GFF4649 0.85 +0.5 59,130 + -0.4 59,130 + -0.9 59,229 + -0.0 59,378 - GFF4650 0.15 -0.6 59,452 + GFF4650 0.26 +0.2 59,513 - GFF4650 0.36 -0.4 59,561 - GFF4650 0.44 -0.3 59,761 + GFF4650 0.75 +0.5 59,761 + GFF4650 0.75 +0.7 59,849 - GFF4650 0.89 +0.1 59,849 - GFF4650 0.89 -0.4 59,961 + +0.2 59,962 - +0.4 60,189 + -0.9 60,189 + +0.5 60,189 + -0.3 60,190 - -1.2 60,190 - -1.8 60,190 - -2.8 60,190 - -0.9
Or see this region's nucleotide sequence