Strain Fitness in Xanthobacter sp. DMC5 around GFF4545

Experiment: Methanol and casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4543 and GFF4544 overlap by 4 nucleotidesGFF4544 and GFF4545 are separated by 68 nucleotidesGFF4545 and GFF4546 are separated by 15 nucleotidesGFF4546 and GFF4547 overlap by 4 nucleotides GFF4543 - Shikimate dehydrogenase (NADP(+)), at 123,056 to 123,859 GFF4543 GFF4544 - 2-oxo-hept-4-ene-1,7-dioate hydratase, at 123,856 to 124,644 GFF4544 GFF4545 - hypothetical protein, at 124,713 to 126,686 GFF4545 GFF4546 - Short-chain-enoyl-CoA hydratase, at 126,702 to 127,493 GFF4546 GFF4547 - 2-hydroxy-3-oxopropionate reductase, at 127,490 to 128,401 GFF4547 Position (kb) 124 125 126 127Strain fitness (log2 ratio) -2 -1 0 1at 123.828 kb on - strandat 123.982 kb on + strand, within GFF4544at 123.982 kb on + strand, within GFF4544at 123.983 kb on - strand, within GFF4544at 123.983 kb on - strand, within GFF4544at 123.983 kb on - strand, within GFF4544at 123.983 kb on - strand, within GFF4544at 123.983 kb on - strand, within GFF4544at 123.983 kb on - strand, within GFF4544at 124.035 kb on + strand, within GFF4544at 124.226 kb on - strand, within GFF4544at 124.508 kb on + strand, within GFF4544at 124.509 kb on - strand, within GFF4544at 124.509 kb on - strand, within GFF4544at 124.509 kb on - strand, within GFF4544at 124.647 kb on + strandat 124.647 kb on + strandat 124.653 kb on - strandat 124.721 kb on - strandat 124.752 kb on + strandat 124.752 kb on + strandat 124.753 kb on - strandat 124.782 kb on + strandat 124.782 kb on + strandat 124.783 kb on - strandat 124.841 kb on + strandat 125.104 kb on - strand, within GFF4545at 125.114 kb on + strand, within GFF4545at 125.175 kb on + strand, within GFF4545at 125.235 kb on + strand, within GFF4545at 125.290 kb on - strand, within GFF4545at 125.301 kb on + strand, within GFF4545at 125.301 kb on + strand, within GFF4545at 125.302 kb on - strand, within GFF4545at 125.302 kb on - strand, within GFF4545at 125.318 kb on - strand, within GFF4545at 125.328 kb on - strand, within GFF4545at 125.328 kb on - strand, within GFF4545at 125.340 kb on + strand, within GFF4545at 125.340 kb on + strand, within GFF4545at 125.341 kb on - strand, within GFF4545at 125.341 kb on - strand, within GFF4545at 125.398 kb on + strand, within GFF4545at 125.398 kb on + strand, within GFF4545at 125.398 kb on + strand, within GFF4545at 125.399 kb on - strand, within GFF4545at 125.399 kb on - strand, within GFF4545at 125.499 kb on - strand, within GFF4545at 125.525 kb on + strand, within GFF4545at 125.538 kb on - strand, within GFF4545at 125.622 kb on - strand, within GFF4545at 125.710 kb on - strand, within GFF4545at 125.711 kb on - strand, within GFF4545at 125.738 kb on + strand, within GFF4545at 125.740 kb on + strand, within GFF4545at 125.740 kb on + strand, within GFF4545at 125.793 kb on + strand, within GFF4545at 125.794 kb on - strand, within GFF4545at 125.794 kb on - strand, within GFF4545at 125.821 kb on + strand, within GFF4545at 125.821 kb on + strand, within GFF4545at 125.879 kb on + strand, within GFF4545at 125.886 kb on + strand, within GFF4545at 125.918 kb on + strand, within GFF4545at 125.995 kb on - strand, within GFF4545at 126.073 kb on + strand, within GFF4545at 126.107 kb on - strand, within GFF4545at 126.136 kb on + strand, within GFF4545at 126.176 kb on + strand, within GFF4545at 126.224 kb on - strand, within GFF4545at 126.300 kb on - strand, within GFF4545at 126.376 kb on + strand, within GFF4545at 126.376 kb on + strand, within GFF4545at 126.405 kb on - strand, within GFF4545at 126.557 kb on + strandat 126.558 kb on - strandat 126.577 kb on - strandat 126.591 kb on - strandat 126.606 kb on - strandat 127.211 kb on - strand, within GFF4546at 127.684 kb on - strand, within GFF4547at 127.684 kb on - strand, within GFF4547

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol and casaminos (C)
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123,828 - -2.7
123,982 + GFF4544 0.16 +1.3
123,982 + GFF4544 0.16 -0.1
123,983 - GFF4544 0.16 +0.4
123,983 - GFF4544 0.16 -1.3
123,983 - GFF4544 0.16 -0.6
123,983 - GFF4544 0.16 -0.1
123,983 - GFF4544 0.16 -0.2
123,983 - GFF4544 0.16 -0.8
124,035 + GFF4544 0.23 -1.8
124,226 - GFF4544 0.47 -1.2
124,508 + GFF4544 0.83 +0.3
124,509 - GFF4544 0.83 -0.7
124,509 - GFF4544 0.83 +0.1
124,509 - GFF4544 0.83 -0.2
124,647 + -1.0
124,647 + -0.3
124,653 - +0.9
124,721 - +0.9
124,752 + +0.1
124,752 + -0.6
124,753 - -0.2
124,782 + -0.4
124,782 + +0.0
124,783 - -0.2
124,841 + +0.1
125,104 - GFF4545 0.20 -0.2
125,114 + GFF4545 0.20 -0.0
125,175 + GFF4545 0.23 +1.0
125,235 + GFF4545 0.26 -0.0
125,290 - GFF4545 0.29 -0.2
125,301 + GFF4545 0.30 -1.2
125,301 + GFF4545 0.30 -0.0
125,302 - GFF4545 0.30 -0.1
125,302 - GFF4545 0.30 -0.6
125,318 - GFF4545 0.31 +0.1
125,328 - GFF4545 0.31 +0.1
125,328 - GFF4545 0.31 -0.5
125,340 + GFF4545 0.32 -0.0
125,340 + GFF4545 0.32 +0.1
125,341 - GFF4545 0.32 -0.4
125,341 - GFF4545 0.32 +0.4
125,398 + GFF4545 0.35 +0.0
125,398 + GFF4545 0.35 -1.7
125,398 + GFF4545 0.35 -0.6
125,399 - GFF4545 0.35 -1.1
125,399 - GFF4545 0.35 -1.2
125,499 - GFF4545 0.40 -1.1
125,525 + GFF4545 0.41 -1.1
125,538 - GFF4545 0.42 +0.9
125,622 - GFF4545 0.46 +0.2
125,710 - GFF4545 0.51 -0.2
125,711 - GFF4545 0.51 +0.6
125,738 + GFF4545 0.52 -1.3
125,740 + GFF4545 0.52 +0.1
125,740 + GFF4545 0.52 -0.5
125,793 + GFF4545 0.55 +0.3
125,794 - GFF4545 0.55 -0.3
125,794 - GFF4545 0.55 +1.3
125,821 + GFF4545 0.56 -0.4
125,821 + GFF4545 0.56 -0.9
125,879 + GFF4545 0.59 -0.3
125,886 + GFF4545 0.59 +1.0
125,918 + GFF4545 0.61 -0.2
125,995 - GFF4545 0.65 +0.2
126,073 + GFF4545 0.69 -0.6
126,107 - GFF4545 0.71 +0.7
126,136 + GFF4545 0.72 -0.3
126,176 + GFF4545 0.74 +0.0
126,224 - GFF4545 0.77 +0.1
126,300 - GFF4545 0.80 +0.8
126,376 + GFF4545 0.84 +0.6
126,376 + GFF4545 0.84 -0.5
126,405 - GFF4545 0.86 -0.0
126,557 + -0.5
126,558 - +0.2
126,577 - +0.1
126,591 - +0.3
126,606 - +0.4
127,211 - GFF4546 0.64 +0.5
127,684 - GFF4547 0.21 -0.6
127,684 - GFF4547 0.21 -1.0

Or see this region's nucleotide sequence