Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF44 and GFF45 are separated by 484 nucleotides GFF45 and GFF46 are separated by 9 nucleotides GFF46 and GFF47 overlap by 4 nucleotides
GFF44 - HTH-type transcriptional regulator GltR, at 52,795 to 53,715
GFF44
GFF45 - Spermidine/putrescine import ATP-binding protein PotA, at 54,200 to 55,378
GFF45
GFF46 - Putrescine transport system permease protein PotH, at 55,388 to 56,305
GFF46
GFF47 - Inner membrane ABC transporter permease protein YdcV, at 56,302 to 57,117
GFF47
Position (kb)
54
55
56 Strain fitness (log2 ratio)
-1
0
1 at 53.231 kb on + strand, within GFF44 at 53.231 kb on + strand, within GFF44 at 53.231 kb on + strand, within GFF44 at 53.231 kb on + strand, within GFF44 at 53.232 kb on - strand, within GFF44 at 53.232 kb on - strand, within GFF44 at 53.232 kb on - strand, within GFF44 at 53.411 kb on + strand, within GFF44 at 53.412 kb on - strand, within GFF44 at 53.412 kb on - strand, within GFF44 at 53.428 kb on - strand, within GFF44 at 53.826 kb on - strand at 53.826 kb on - strand at 53.844 kb on + strand at 53.879 kb on + strand at 53.880 kb on - strand at 53.882 kb on - strand at 54.087 kb on + strand at 54.365 kb on - strand, within GFF45 at 54.368 kb on + strand, within GFF45 at 54.368 kb on + strand, within GFF45 at 54.368 kb on + strand, within GFF45 at 54.368 kb on + strand, within GFF45 at 54.369 kb on - strand, within GFF45 at 54.369 kb on - strand, within GFF45 at 54.369 kb on - strand, within GFF45 at 54.369 kb on - strand, within GFF45 at 54.953 kb on + strand, within GFF45 at 54.974 kb on + strand, within GFF45 at 54.974 kb on + strand, within GFF45 at 54.974 kb on + strand, within GFF45 at 54.975 kb on - strand, within GFF45 at 54.975 kb on - strand, within GFF45 at 54.975 kb on - strand, within GFF45 at 55.238 kb on + strand, within GFF45 at 55.238 kb on + strand, within GFF45 at 55.238 kb on + strand, within GFF45 at 55.238 kb on + strand, within GFF45 at 55.239 kb on - strand, within GFF45 at 55.376 kb on + strand at 55.376 kb on + strand at 55.377 kb on - strand at 55.442 kb on + strand at 55.443 kb on - strand at 55.443 kb on - strand at 55.529 kb on + strand, within GFF46 at 55.604 kb on + strand, within GFF46 at 55.610 kb on + strand, within GFF46 at 55.611 kb on - strand, within GFF46 at 55.611 kb on - strand, within GFF46 at 55.646 kb on + strand, within GFF46 at 55.715 kb on + strand, within GFF46 at 55.716 kb on - strand, within GFF46 at 55.913 kb on + strand, within GFF46 at 55.914 kb on - strand, within GFF46 at 55.928 kb on + strand, within GFF46 at 55.928 kb on + strand, within GFF46 at 55.935 kb on - strand, within GFF46 at 55.962 kb on - strand, within GFF46 at 55.962 kb on - strand, within GFF46 at 56.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 53,231 + GFF44 0.47 +0.6 53,231 + GFF44 0.47 +1.3 53,231 + GFF44 0.47 +0.6 53,231 + GFF44 0.47 -0.0 53,232 - GFF44 0.47 +0.1 53,232 - GFF44 0.47 -0.6 53,232 - GFF44 0.47 -0.4 53,411 + GFF44 0.67 -0.4 53,412 - GFF44 0.67 +0.3 53,412 - GFF44 0.67 -0.5 53,428 - GFF44 0.69 +0.0 53,826 - +1.0 53,826 - +0.3 53,844 + +0.1 53,879 + +0.2 53,880 - +0.4 53,882 - -0.4 54,087 + +1.0 54,365 - GFF45 0.14 -0.4 54,368 + GFF45 0.14 -0.8 54,368 + GFF45 0.14 -0.5 54,368 + GFF45 0.14 -0.9 54,368 + GFF45 0.14 -1.4 54,369 - GFF45 0.14 -0.6 54,369 - GFF45 0.14 -0.0 54,369 - GFF45 0.14 -0.7 54,369 - GFF45 0.14 -0.7 54,953 + GFF45 0.64 -0.2 54,974 + GFF45 0.66 -0.8 54,974 + GFF45 0.66 -0.7 54,974 + GFF45 0.66 -0.7 54,975 - GFF45 0.66 -1.0 54,975 - GFF45 0.66 +0.4 54,975 - GFF45 0.66 -0.6 55,238 + GFF45 0.88 -0.2 55,238 + GFF45 0.88 -0.3 55,238 + GFF45 0.88 +0.1 55,238 + GFF45 0.88 +0.4 55,239 - GFF45 0.88 +0.4 55,376 + -1.4 55,376 + -0.4 55,377 - -0.7 55,442 + -1.6 55,443 - -0.4 55,443 - -0.6 55,529 + GFF46 0.15 -0.8 55,604 + GFF46 0.24 +0.5 55,610 + GFF46 0.24 +0.2 55,611 - GFF46 0.24 +1.2 55,611 - GFF46 0.24 +0.4 55,646 + GFF46 0.28 -0.2 55,715 + GFF46 0.36 -0.3 55,716 - GFF46 0.36 -0.3 55,913 + GFF46 0.57 -0.3 55,914 - GFF46 0.57 -0.9 55,928 + GFF46 0.59 -0.1 55,928 + GFF46 0.59 -0.8 55,935 - GFF46 0.60 -0.1 55,962 - GFF46 0.63 -0.6 55,962 - GFF46 0.63 -1.5 56,265 - -0.1
Or see this region's nucleotide sequence