Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4460 and GFF4461 overlap by 4 nucleotides GFF4461 and GFF4462 are separated by 4 nucleotides GFF4462 and GFF4463 are separated by 106 nucleotides GFF4463 and GFF4464 are separated by 147 nucleotides
GFF4460 - hypothetical protein, at 40,563 to 40,748
GFF4460
GFF4461 - hypothetical protein, at 40,745 to 41,566
GFF4461
GFF4462 - RNA pyrophosphohydrolase, at 41,571 to 42,104
GFF4462
GFF4463 - hypothetical protein, at 42,211 to 42,840
GFF4463
GFF4464 - 50S ribosomal protein L27, at 42,988 to 43,260
GFF4464
Position (kb) 41
42
43 Strain fitness (log2 ratio) -1
0
1
2 at 40.875 kb on - strand, within GFF4461 at 40.875 kb on - strand, within GFF4461 at 41.185 kb on - strand, within GFF4461 at 41.186 kb on + strand, within GFF4461 at 41.187 kb on - strand, within GFF4461 at 41.187 kb on - strand, within GFF4461 at 41.309 kb on + strand, within GFF4461 at 41.310 kb on - strand, within GFF4461 at 41.310 kb on - strand, within GFF4461 at 41.614 kb on + strand at 41.614 kb on + strand at 41.614 kb on + strand at 41.615 kb on - strand at 41.827 kb on + strand, within GFF4462 at 41.827 kb on + strand, within GFF4462 at 41.828 kb on - strand, within GFF4462 at 41.828 kb on - strand, within GFF4462 at 41.828 kb on - strand, within GFF4462 at 41.887 kb on + strand, within GFF4462 at 41.887 kb on + strand, within GFF4462 at 41.911 kb on + strand, within GFF4462 at 41.911 kb on + strand, within GFF4462 at 41.911 kb on + strand, within GFF4462 at 41.912 kb on - strand, within GFF4462 at 42.446 kb on + strand, within GFF4463 at 42.447 kb on - strand, within GFF4463 at 42.552 kb on - strand, within GFF4463 at 42.668 kb on + strand, within GFF4463 at 42.668 kb on + strand, within GFF4463 at 42.669 kb on - strand, within GFF4463 at 42.669 kb on - strand, within GFF4463 at 42.747 kb on + strand, within GFF4463 at 42.747 kb on + strand, within GFF4463 at 42.792 kb on + strand at 42.793 kb on - strand at 42.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 40,875 - GFF4461 0.16 -0.1 40,875 - GFF4461 0.16 +0.4 41,185 - GFF4461 0.54 +0.1 41,186 + GFF4461 0.54 +0.7 41,187 - GFF4461 0.54 -0.6 41,187 - GFF4461 0.54 +0.1 41,309 + GFF4461 0.69 +0.2 41,310 - GFF4461 0.69 -0.0 41,310 - GFF4461 0.69 +0.0 41,614 + +0.1 41,614 + -0.1 41,614 + +1.1 41,615 - -0.9 41,827 + GFF4462 0.48 -0.1 41,827 + GFF4462 0.48 -0.2 41,828 - GFF4462 0.48 -0.3 41,828 - GFF4462 0.48 -1.2 41,828 - GFF4462 0.48 -0.6 41,887 + GFF4462 0.59 -0.8 41,887 + GFF4462 0.59 -0.0 41,911 + GFF4462 0.64 -0.1 41,911 + GFF4462 0.64 -1.7 41,911 + GFF4462 0.64 -0.8 41,912 - GFF4462 0.64 -0.5 42,446 + GFF4463 0.37 +0.9 42,447 - GFF4463 0.37 +1.4 42,552 - GFF4463 0.54 +1.2 42,668 + GFF4463 0.73 +2.7 42,668 + GFF4463 0.73 -0.3 42,669 - GFF4463 0.73 +1.1 42,669 - GFF4463 0.73 +1.1 42,747 + GFF4463 0.85 +0.7 42,747 + GFF4463 0.85 +0.0 42,792 + +0.1 42,793 - -0.2 42,793 - +0.6
Or see this region's nucleotide sequence