Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4412 and GFF4413 are separated by 39 nucleotides GFF4413 and GFF4414 are separated by 3 nucleotides GFF4414 and GFF4415 are separated by 381 nucleotides
GFF4412 - Nitrogenase-stabilizing/protective protein NifW, at 219,641 to 219,982
GFF4412
GFF4413 - Serine acetyltransferase, at 220,022 to 220,801
GFF4413
GFF4414 - 2-phosphonomethylmalate synthase, at 220,805 to 222,052
GFF4414
GFF4415 - Nicotinate-nucleotide pyrophosphorylase [carboxylating], at 222,434 to 223,276
GFF4415
Position (kb) 220
221
222
223 Strain fitness (log2 ratio) -1
0
1 at 219.810 kb on + strand, within GFF4412 at 219.811 kb on - strand, within GFF4412 at 219.811 kb on - strand, within GFF4412 at 219.855 kb on + strand, within GFF4412 at 219.856 kb on - strand, within GFF4412 at 219.856 kb on - strand, within GFF4412 at 219.856 kb on - strand, within GFF4412 at 219.912 kb on + strand, within GFF4412 at 220.421 kb on + strand, within GFF4413 at 220.630 kb on - strand, within GFF4413 at 220.772 kb on - strand at 220.849 kb on - strand at 220.866 kb on + strand at 220.866 kb on + strand at 220.867 kb on - strand at 221.070 kb on + strand, within GFF4414 at 221.071 kb on - strand, within GFF4414 at 221.440 kb on - strand, within GFF4414 at 221.440 kb on - strand, within GFF4414 at 221.697 kb on + strand, within GFF4414 at 221.698 kb on - strand, within GFF4414 at 222.068 kb on + strand at 222.079 kb on + strand at 222.079 kb on + strand at 222.079 kb on + strand at 222.079 kb on + strand at 222.080 kb on - strand at 222.080 kb on - strand at 222.080 kb on - strand at 222.215 kb on + strand at 222.327 kb on + strand at 222.327 kb on + strand at 222.327 kb on + strand at 222.328 kb on - strand at 222.328 kb on - strand at 222.328 kb on - strand at 222.330 kb on - strand at 222.330 kb on - strand at 222.481 kb on - strand at 222.481 kb on - strand at 222.966 kb on + strand, within GFF4415
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 219,810 + GFF4412 0.49 +0.4 219,811 - GFF4412 0.50 -0.7 219,811 - GFF4412 0.50 -1.6 219,855 + GFF4412 0.63 +0.0 219,856 - GFF4412 0.63 -0.2 219,856 - GFF4412 0.63 +0.5 219,856 - GFF4412 0.63 -0.6 219,912 + GFF4412 0.79 -0.6 220,421 + GFF4413 0.51 -0.9 220,630 - GFF4413 0.78 +0.8 220,772 - -0.7 220,849 - +0.2 220,866 + -0.2 220,866 + +1.1 220,867 - -0.1 221,070 + GFF4414 0.21 -0.7 221,071 - GFF4414 0.21 +0.3 221,440 - GFF4414 0.51 -0.6 221,440 - GFF4414 0.51 +0.2 221,697 + GFF4414 0.71 -0.3 221,698 - GFF4414 0.72 -0.7 222,068 + +0.2 222,079 + -0.5 222,079 + -0.9 222,079 + +0.2 222,079 + +0.6 222,080 - +0.7 222,080 - -0.5 222,080 - -0.5 222,215 + -0.3 222,327 + -0.4 222,327 + +0.4 222,327 + +0.6 222,328 - -0.4 222,328 - +0.2 222,328 - +0.3 222,330 - +1.2 222,330 - -0.6 222,481 - -0.8 222,481 - -0.1 222,966 + GFF4415 0.63 +0.2
Or see this region's nucleotide sequence