Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF414 and GFF415 are separated by 13 nucleotides GFF415 and GFF416 are separated by 160 nucleotides GFF416 and GFF417 are separated by 164 nucleotides
GFF414 - DNA-3-methyladenine glycosylase, at 425,842 to 426,492
GFF414
GFF415 - hypothetical protein, at 426,506 to 427,210
GFF415
GFF416 - hypothetical protein, at 427,371 to 427,928
GFF416
GFF417 - 3-mercaptopyruvate sulfurtransferase, at 428,093 to 428,965
GFF417
Position (kb) 427
428 Strain fitness (log2 ratio) -2
-1
0
1 at 426.452 kb on - strand at 426.452 kb on - strand at 426.455 kb on - strand at 426.617 kb on + strand, within GFF415 at 426.839 kb on + strand, within GFF415 at 426.839 kb on + strand, within GFF415 at 426.840 kb on - strand, within GFF415 at 427.246 kb on + strand at 427.305 kb on + strand at 427.306 kb on - strand at 427.306 kb on - strand at 427.306 kb on - strand at 427.440 kb on + strand, within GFF416 at 427.515 kb on + strand, within GFF416 at 427.516 kb on - strand, within GFF416 at 427.518 kb on + strand, within GFF416 at 427.656 kb on + strand, within GFF416 at 427.778 kb on + strand, within GFF416 at 427.779 kb on - strand, within GFF416 at 427.872 kb on + strand, within GFF416 at 427.897 kb on - strand at 428.656 kb on - strand, within GFF417 at 428.727 kb on - strand, within GFF417 at 428.808 kb on + strand, within GFF417
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 426,452 - +0.0 426,452 - -0.4 426,455 - -1.1 426,617 + GFF415 0.16 +0.5 426,839 + GFF415 0.47 +0.2 426,839 + GFF415 0.47 -0.1 426,840 - GFF415 0.47 +0.5 427,246 + -0.3 427,305 + +1.0 427,306 - +0.0 427,306 - -0.8 427,306 - -0.2 427,440 + GFF416 0.12 -0.5 427,515 + GFF416 0.26 -0.2 427,516 - GFF416 0.26 +0.7 427,518 + GFF416 0.26 +0.0 427,656 + GFF416 0.51 -2.3 427,778 + GFF416 0.73 -2.4 427,779 - GFF416 0.73 -0.4 427,872 + GFF416 0.90 -1.2 427,897 - -0.4 428,656 - GFF417 0.64 -0.6 428,727 - GFF417 0.73 -0.0 428,808 + GFF417 0.82 -0.0
Or see this region's nucleotide sequence