Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3953 and GFF3954 are separated by 135 nucleotides GFF3954 and GFF3955 are separated by 33 nucleotides GFF3955 and GFF3956 are separated by 454 nucleotides
GFF3953 - hypothetical protein, at 319,787 to 320,719
GFF3953
GFF3954 - hypothetical protein, at 320,855 to 321,184
GFF3954
GFF3955 - hypothetical protein, at 321,218 to 322,654
GFF3955
GFF3956 - hypothetical protein, at 323,109 to 325,109
GFF3956
Position (kb) 321
322
323 Strain fitness (log2 ratio) -1
0
1 at 320.224 kb on - strand, within GFF3953 at 320.559 kb on - strand, within GFF3953 at 320.684 kb on - strand at 321.238 kb on - strand at 321.238 kb on - strand at 321.238 kb on - strand at 321.264 kb on + strand at 321.265 kb on - strand at 321.265 kb on - strand at 321.406 kb on - strand, within GFF3955 at 321.527 kb on - strand, within GFF3955 at 321.606 kb on + strand, within GFF3955 at 321.679 kb on + strand, within GFF3955 at 321.815 kb on - strand, within GFF3955 at 322.204 kb on - strand, within GFF3955 at 322.718 kb on - strand at 323.207 kb on - strand at 323.260 kb on + strand at 323.261 kb on - strand at 323.374 kb on - strand, within GFF3956 at 323.425 kb on + strand, within GFF3956 at 323.476 kb on + strand, within GFF3956 at 323.476 kb on + strand, within GFF3956 at 323.478 kb on + strand, within GFF3956
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 320,224 - GFF3953 0.47 +0.2 320,559 - GFF3953 0.83 +0.1 320,684 - +0.1 321,238 - -0.3 321,238 - +1.0 321,238 - +0.0 321,264 + +0.5 321,265 - -0.1 321,265 - +0.1 321,406 - GFF3955 0.13 +0.8 321,527 - GFF3955 0.22 +1.2 321,606 + GFF3955 0.27 -0.8 321,679 + GFF3955 0.32 -0.7 321,815 - GFF3955 0.42 +0.7 322,204 - GFF3955 0.69 +0.5 322,718 - +0.5 323,207 - -1.1 323,260 + +0.9 323,261 - -0.0 323,374 - GFF3956 0.13 -1.0 323,425 + GFF3956 0.16 +0.7 323,476 + GFF3956 0.18 -0.5 323,476 + GFF3956 0.18 +0.4 323,478 + GFF3956 0.18 -0.5
Or see this region's nucleotide sequence