Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3696 and GFF3697 are separated by 91 nucleotides GFF3697 and GFF3698 are separated by 13 nucleotides GFF3698 and GFF3699 are separated by 18 nucleotides
GFF3696 - NAD(P)-dependent methylenetetrahydromethanopterin dehydrogenase, at 39,343 to 40,245
GFF3696
GFF3697 - Methenyltetrahydromethanopterin cyclohydrolase, at 40,337 to 41,347
GFF3697
GFF3698 - hypothetical protein, at 41,361 to 42,245
GFF3698
GFF3699 - hypothetical protein, at 42,264 to 43,214
GFF3699
Position (kb) 40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 39.360 kb on + strand at 39.360 kb on + strand at 39.628 kb on + strand, within GFF3696 at 39.629 kb on - strand, within GFF3696 at 39.745 kb on + strand, within GFF3696 at 41.210 kb on + strand, within GFF3697 at 41.211 kb on - strand, within GFF3697 at 41.211 kb on - strand, within GFF3697 at 41.226 kb on - strand, within GFF3697 at 41.866 kb on - strand, within GFF3698 at 41.866 kb on - strand, within GFF3698 at 41.869 kb on - strand, within GFF3698 at 41.869 kb on - strand, within GFF3698 at 42.285 kb on + strand at 42.285 kb on + strand at 42.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 39,360 + +0.4 39,360 + -0.1 39,628 + GFF3696 0.32 +0.2 39,629 - GFF3696 0.32 +0.8 39,745 + GFF3696 0.45 +0.8 41,210 + GFF3697 0.86 -0.6 41,211 - GFF3697 0.86 -0.3 41,211 - GFF3697 0.86 -0.8 41,226 - GFF3697 0.88 -1.1 41,866 - GFF3698 0.57 -2.4 41,866 - GFF3698 0.57 -2.8 41,869 - GFF3698 0.57 -2.1 41,869 - GFF3698 0.57 -1.1 42,285 + -0.3 42,285 + -2.0 42,286 - -3.0
Or see this region's nucleotide sequence