Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3542 and GFF3543 are separated by 1 nucleotides GFF3543 and GFF3544 are separated by 94 nucleotides GFF3544 and GFF3545 overlap by 70 nucleotides
GFF3542 - Polyphosphate kinase, at 397,007 to 399,193
GFF3542
GFF3543 - Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 399,195 to 400,721
GFF3543
GFF3544 - hypothetical protein, at 400,816 to 401,115
GFF3544
GFF3545 - hypothetical protein, at 401,046 to 401,900
GFF3545
Position (kb) 399
400
401 Strain fitness (log2 ratio) -2
-1
0
1
2 at 399.098 kb on + strand at 399.099 kb on - strand at 399.279 kb on + strand at 399.279 kb on + strand at 399.666 kb on + strand, within GFF3543 at 399.761 kb on + strand, within GFF3543 at 399.761 kb on + strand, within GFF3543 at 399.807 kb on + strand, within GFF3543 at 399.811 kb on - strand, within GFF3543 at 400.095 kb on + strand, within GFF3543 at 400.095 kb on + strand, within GFF3543 at 400.392 kb on + strand, within GFF3543 at 400.393 kb on - strand, within GFF3543 at 400.524 kb on + strand, within GFF3543 at 400.524 kb on + strand, within GFF3543 at 400.524 kb on + strand, within GFF3543 at 400.524 kb on + strand, within GFF3543 at 400.525 kb on - strand, within GFF3543 at 400.525 kb on - strand, within GFF3543 at 400.525 kb on - strand, within GFF3543 at 400.625 kb on + strand at 400.626 kb on - strand at 400.960 kb on + strand, within GFF3544 at 400.961 kb on - strand, within GFF3544 at 401.047 kb on + strand at 401.048 kb on - strand at 401.216 kb on - strand, within GFF3545 at 401.431 kb on + strand, within GFF3545 at 401.431 kb on + strand, within GFF3545 at 401.432 kb on - strand, within GFF3545 at 401.450 kb on - strand, within GFF3545 at 401.668 kb on + strand, within GFF3545 at 401.668 kb on + strand, within GFF3545 at 401.668 kb on + strand, within GFF3545 at 401.669 kb on - strand, within GFF3545
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 399,098 + +1.8 399,099 - +0.9 399,279 + -0.7 399,279 + -0.2 399,666 + GFF3543 0.31 -1.4 399,761 + GFF3543 0.37 +0.6 399,761 + GFF3543 0.37 -0.4 399,807 + GFF3543 0.40 -1.3 399,811 - GFF3543 0.40 +0.7 400,095 + GFF3543 0.59 -1.0 400,095 + GFF3543 0.59 +0.1 400,392 + GFF3543 0.78 -0.2 400,393 - GFF3543 0.78 +0.4 400,524 + GFF3543 0.87 +0.1 400,524 + GFF3543 0.87 +0.6 400,524 + GFF3543 0.87 -1.3 400,524 + GFF3543 0.87 -0.2 400,525 - GFF3543 0.87 -0.4 400,525 - GFF3543 0.87 -1.0 400,525 - GFF3543 0.87 -0.6 400,625 + -0.2 400,626 - +0.2 400,960 + GFF3544 0.48 -0.5 400,961 - GFF3544 0.48 +0.0 401,047 + -1.9 401,048 - +0.8 401,216 - GFF3545 0.20 +0.1 401,431 + GFF3545 0.45 +0.1 401,431 + GFF3545 0.45 -0.7 401,432 - GFF3545 0.45 -0.3 401,450 - GFF3545 0.47 +0.3 401,668 + GFF3545 0.73 -1.9 401,668 + GFF3545 0.73 -0.8 401,668 + GFF3545 0.73 +0.9 401,669 - GFF3545 0.73 +0.9
Or see this region's nucleotide sequence