Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3348 and GFF3349 are separated by 117 nucleotides GFF3349 and GFF3350 overlap by 4 nucleotides GFF3350 and GFF3351 are separated by 3 nucleotides
GFF3348 - HTH-type transcriptional regulator GltC, at 192,601 to 193,482
GFF3348
GFF3349 - Lipopolysaccharide export system ATP-binding protein LptB, at 193,600 to 194,433
GFF3349
GFF3350 - Vitamin B12 import ATP-binding protein BtuD, at 194,430 to 195,188
GFF3350
GFF3351 - High-affinity branched-chain amino acid transport system permease protein LivH, at 195,192 to 196,055
GFF3351
Position (kb) 193
194
195 Strain fitness (log2 ratio) -1
0
1
2 at 192.688 kb on + strand at 192.887 kb on + strand, within GFF3348 at 193.166 kb on + strand, within GFF3348 at 193.167 kb on - strand, within GFF3348 at 193.167 kb on - strand, within GFF3348 at 193.709 kb on - strand, within GFF3349 at 194.414 kb on - strand at 194.715 kb on + strand, within GFF3350 at 194.715 kb on + strand, within GFF3350 at 194.715 kb on + strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 195.327 kb on + strand, within GFF3351 at 195.337 kb on - strand, within GFF3351
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 192,688 + -0.2 192,887 + GFF3348 0.32 +0.2 193,166 + GFF3348 0.64 -0.4 193,167 - GFF3348 0.64 -0.9 193,167 - GFF3348 0.64 -0.5 193,709 - GFF3349 0.13 +0.3 194,414 - +1.3 194,715 + GFF3350 0.38 +0.4 194,715 + GFF3350 0.38 -0.1 194,715 + GFF3350 0.38 +1.5 194,716 - GFF3350 0.38 -1.7 194,716 - GFF3350 0.38 +0.3 194,716 - GFF3350 0.38 -0.3 194,716 - GFF3350 0.38 +0.2 194,716 - GFF3350 0.38 +2.0 194,716 - GFF3350 0.38 +0.0 195,327 + GFF3351 0.16 -0.2 195,337 - GFF3351 0.17 -0.2
Or see this region's nucleotide sequence