Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3245 and GFF3246 are separated by 486 nucleotides GFF3246 and GFF3247 overlap by 4 nucleotides GFF3247 and GFF3248 overlap by 8 nucleotides
GFF3245 - Putative cysteine protease YraA, at 75,177 to 75,740
GFF3245
GFF3246 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 76,227 to 76,940
GFF3246
GFF3247 - Lipopolysaccharide export system ATP-binding protein LptB, at 76,937 to 77,686
GFF3247
GFF3248 - hypothetical protein, at 77,679 to 78,596
GFF3248
Position (kb) 76
77 Strain fitness (log2 ratio) -1
0
1 at 75.480 kb on + strand, within GFF3245 at 75.480 kb on + strand, within GFF3245 at 75.570 kb on + strand, within GFF3245 at 75.570 kb on + strand, within GFF3245 at 75.571 kb on - strand, within GFF3245 at 75.820 kb on - strand at 75.937 kb on + strand at 75.938 kb on - strand at 75.938 kb on - strand at 76.158 kb on + strand at 76.158 kb on + strand at 76.158 kb on + strand at 76.158 kb on + strand at 76.159 kb on - strand at 76.241 kb on - strand at 76.540 kb on + strand, within GFF3246 at 76.580 kb on - strand, within GFF3246 at 76.956 kb on + strand at 76.957 kb on - strand at 76.957 kb on - strand at 77.643 kb on + strand at 77.902 kb on + strand, within GFF3248 at 77.902 kb on + strand, within GFF3248 at 77.903 kb on - strand, within GFF3248 at 77.936 kb on - strand, within GFF3248
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 75,480 + GFF3245 0.54 +0.0 75,480 + GFF3245 0.54 -0.6 75,570 + GFF3245 0.70 -0.3 75,570 + GFF3245 0.70 -0.7 75,571 - GFF3245 0.70 -1.3 75,820 - -1.1 75,937 + +0.4 75,938 - -0.6 75,938 - -0.8 76,158 + -0.8 76,158 + -1.1 76,158 + -0.2 76,158 + -0.2 76,159 - -0.4 76,241 - +0.9 76,540 + GFF3246 0.44 +0.3 76,580 - GFF3246 0.49 -0.7 76,956 + -0.5 76,957 - -0.6 76,957 - -0.4 77,643 + +0.1 77,902 + GFF3248 0.24 +0.2 77,902 + GFF3248 0.24 -0.5 77,903 - GFF3248 0.24 -0.6 77,936 - GFF3248 0.28 +0.3
Or see this region's nucleotide sequence