Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3075 and GFF3076 are separated by 22 nucleotides GFF3076 and GFF3077 are separated by 97 nucleotides GFF3077 and GFF3078 overlap by 4 nucleotides
GFF3075 - Endonuclease III, at 807,149 to 808,096
GFF3075
GFF3076 - hypothetical protein, at 808,119 to 808,658
GFF3076
GFF3077 - Anti-sigma-E factor ChrR, at 808,756 to 809,451
GFF3077
GFF3078 - ECF RNA polymerase sigma factor RpoE, at 809,448 to 810,029
GFF3078
Position (kb)
808
809
810 Strain fitness (log2 ratio)
-1
0
1 at 808.108 kb on - strand at 808.188 kb on + strand, within GFF3076 at 808.188 kb on + strand, within GFF3076 at 808.188 kb on + strand, within GFF3076 at 808.189 kb on - strand, within GFF3076 at 808.347 kb on + strand, within GFF3076 at 808.885 kb on + strand, within GFF3077 at 808.885 kb on + strand, within GFF3077 at 808.885 kb on + strand, within GFF3077 at 808.886 kb on - strand, within GFF3077 at 808.904 kb on + strand, within GFF3077 at 809.408 kb on + strand at 809.707 kb on + strand, within GFF3078 at 809.708 kb on - strand, within GFF3078 at 809.711 kb on + strand, within GFF3078 at 809.711 kb on + strand, within GFF3078 at 809.712 kb on - strand, within GFF3078 at 809.807 kb on - strand, within GFF3078 at 809.891 kb on - strand, within GFF3078 at 809.891 kb on - strand, within GFF3078
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 808,108 - +0.3 808,188 + GFF3076 0.13 +0.7 808,188 + GFF3076 0.13 -0.9 808,188 + GFF3076 0.13 +0.1 808,189 - GFF3076 0.13 +0.1 808,347 + GFF3076 0.42 -0.6 808,885 + GFF3077 0.19 +0.4 808,885 + GFF3077 0.19 +0.4 808,885 + GFF3077 0.19 -1.1 808,886 - GFF3077 0.19 -0.1 808,904 + GFF3077 0.21 +1.0 809,408 + +0.2 809,707 + GFF3078 0.45 +0.9 809,708 - GFF3078 0.45 -1.5 809,711 + GFF3078 0.45 +0.3 809,711 + GFF3078 0.45 -0.0 809,712 - GFF3078 0.45 +0.0 809,807 - GFF3078 0.62 -0.0 809,891 - GFF3078 0.76 -0.1 809,891 - GFF3078 0.76 -0.8
Or see this region's nucleotide sequence