Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF300 and GFF301 overlap by 4 nucleotides GFF301 and GFF302 overlap by 4 nucleotides GFF302 and GFF303 are separated by 3 nucleotides GFF303 and GFF304 are separated by 1 nucleotides GFF304 and GFF305 overlap by 8 nucleotides GFF305 and GFF306 are separated by 151 nucleotides
GFF300 - ATP-dependent zinc metalloprotease FtsH, at 313,672 to 314,595
GFF300
GFF301 - hypothetical protein, at 314,592 to 315,494
GFF301
GFF302 - hypothetical protein, at 315,491 to 316,069
GFF302
GFF303 - hypothetical protein, at 316,073 to 316,333
GFF303
GFF304 - hypothetical protein, at 316,335 to 316,610
GFF304
GFF305 - hypothetical protein, at 316,603 to 316,866
GFF305
GFF306 - hypothetical protein, at 317,018 to 317,329
GFF306
Position (kb) 315
316
317 Strain fitness (log2 ratio) -2
-1
0
1 at 314.545 kb on - strand at 314.545 kb on - strand at 314.907 kb on + strand, within GFF301 at 314.907 kb on + strand, within GFF301 at 314.907 kb on + strand, within GFF301 at 315.084 kb on - strand, within GFF301 at 315.085 kb on + strand, within GFF301 at 315.086 kb on - strand, within GFF301 at 315.283 kb on + strand, within GFF301 at 315.283 kb on + strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.284 kb on - strand, within GFF301 at 315.461 kb on - strand at 315.461 kb on - strand at 315.526 kb on + strand at 315.526 kb on + strand at 315.921 kb on + strand, within GFF302 at 315.921 kb on + strand, within GFF302 at 315.921 kb on + strand, within GFF302 at 316.017 kb on + strand at 316.017 kb on + strand at 316.018 kb on - strand at 316.260 kb on + strand, within GFF303 at 316.495 kb on + strand, within GFF304 at 316.495 kb on + strand, within GFF304 at 316.688 kb on + strand, within GFF305 at 317.018 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 314,545 - -0.7 314,545 - -0.2 314,907 + GFF301 0.35 +0.8 314,907 + GFF301 0.35 -1.9 314,907 + GFF301 0.35 -0.9 315,084 - GFF301 0.54 +0.1 315,085 + GFF301 0.55 -0.2 315,086 - GFF301 0.55 +0.3 315,283 + GFF301 0.77 +0.1 315,283 + GFF301 0.77 -0.2 315,284 - GFF301 0.77 -0.9 315,284 - GFF301 0.77 -1.2 315,284 - GFF301 0.77 -0.2 315,284 - GFF301 0.77 -0.3 315,461 - -0.6 315,461 - -0.1 315,526 + -0.1 315,526 + +0.0 315,921 + GFF302 0.74 +0.1 315,921 + GFF302 0.74 +0.5 315,921 + GFF302 0.74 +0.3 316,017 + -0.6 316,017 + -0.7 316,018 - -0.5 316,260 + GFF303 0.72 -0.1 316,495 + GFF304 0.58 -0.0 316,495 + GFF304 0.58 -0.5 316,688 + GFF305 0.32 +0.2 317,018 - -0.1
Or see this region's nucleotide sequence