Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3016 and GFF3017 are separated by 147 nucleotides GFF3017 and GFF3018 are separated by 19 nucleotides GFF3018 and GFF3019 are separated by 64 nucleotides GFF3019 and GFF3020 are separated by 135 nucleotides
GFF3016 - Leu/Ile/Val-binding protein, at 740,543 to 741,793
GFF3016
GFF3017 - HTH-type transcriptional repressor RspR, at 741,941 to 742,624
GFF3017
GFF3018 - NAD/NADP-dependent betaine aldehyde dehydrogenase, at 742,644 to 744,164
GFF3018
GFF3019 - hypothetical protein, at 744,229 to 745,023
GFF3019
GFF3020 - Virginiamycin B lyase, at 745,159 to 746,091
GFF3020
Position (kb) 742
743
744
745 Strain fitness (log2 ratio) -2
-1
0
1 at 741.970 kb on + strand at 742.010 kb on + strand, within GFF3017 at 742.343 kb on + strand, within GFF3017 at 742.923 kb on + strand, within GFF3018 at 742.924 kb on - strand, within GFF3018 at 743.005 kb on - strand, within GFF3018 at 743.061 kb on + strand, within GFF3018 at 743.061 kb on + strand, within GFF3018 at 743.070 kb on + strand, within GFF3018 at 743.070 kb on + strand, within GFF3018 at 743.071 kb on - strand, within GFF3018 at 743.451 kb on + strand, within GFF3018 at 743.451 kb on + strand, within GFF3018 at 743.451 kb on + strand, within GFF3018 at 743.452 kb on - strand, within GFF3018 at 743.475 kb on + strand, within GFF3018 at 743.475 kb on + strand, within GFF3018 at 743.553 kb on + strand, within GFF3018 at 743.553 kb on + strand, within GFF3018 at 743.554 kb on - strand, within GFF3018 at 743.554 kb on - strand, within GFF3018 at 743.554 kb on - strand, within GFF3018 at 743.554 kb on - strand, within GFF3018 at 744.130 kb on - strand at 744.130 kb on - strand at 744.185 kb on + strand at 744.186 kb on - strand at 744.308 kb on + strand at 744.379 kb on + strand, within GFF3019 at 744.710 kb on + strand, within GFF3019 at 744.799 kb on - strand, within GFF3019 at 744.813 kb on - strand, within GFF3019 at 745.109 kb on + strand at 745.109 kb on + strand at 745.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 741,970 + +0.6 742,010 + GFF3017 0.10 -2.2 742,343 + GFF3017 0.59 +0.9 742,923 + GFF3018 0.18 +0.5 742,924 - GFF3018 0.18 -1.2 743,005 - GFF3018 0.24 -0.4 743,061 + GFF3018 0.27 +0.1 743,061 + GFF3018 0.27 -0.1 743,070 + GFF3018 0.28 -0.0 743,070 + GFF3018 0.28 -0.3 743,071 - GFF3018 0.28 +0.5 743,451 + GFF3018 0.53 +0.6 743,451 + GFF3018 0.53 -0.7 743,451 + GFF3018 0.53 -0.2 743,452 - GFF3018 0.53 -0.5 743,475 + GFF3018 0.55 -0.2 743,475 + GFF3018 0.55 -1.0 743,553 + GFF3018 0.60 -0.8 743,553 + GFF3018 0.60 +0.8 743,554 - GFF3018 0.60 +0.3 743,554 - GFF3018 0.60 -0.3 743,554 - GFF3018 0.60 -1.2 743,554 - GFF3018 0.60 +0.3 744,130 - +0.1 744,130 - -0.1 744,185 + -0.1 744,186 - +0.6 744,308 + -0.7 744,379 + GFF3019 0.19 -0.4 744,710 + GFF3019 0.61 +0.2 744,799 - GFF3019 0.72 +0.4 744,813 - GFF3019 0.73 +0.2 745,109 + -0.2 745,109 + +0.3 745,112 + -1.2
Or see this region's nucleotide sequence