Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3012 and GFF3013 overlap by 8 nucleotides GFF3013 and GFF3014 overlap by 8 nucleotides GFF3014 and GFF3015 overlap by 4 nucleotides
GFF3012 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 737,011 to 737,727
GFF3012
GFF3013 - Sulfate/thiosulfate import ATP-binding protein CysA, at 737,720 to 738,463
GFF3013
GFF3014 - hypothetical protein, at 738,456 to 739,442
GFF3014
GFF3015 - High-affinity branched-chain amino acid transport system permease protein LivH, at 739,439 to 740,299
GFF3015
Position (kb) 738
739
740 Strain fitness (log2 ratio) -2
-1
0
1 at 737.544 kb on - strand, within GFF3012 at 737.733 kb on + strand at 737.733 kb on + strand at 737.734 kb on - strand at 737.734 kb on - strand at 737.841 kb on + strand, within GFF3013 at 738.207 kb on + strand, within GFF3013 at 738.207 kb on + strand, within GFF3013 at 738.208 kb on - strand, within GFF3013 at 738.767 kb on - strand, within GFF3014 at 738.775 kb on + strand, within GFF3014 at 738.775 kb on + strand, within GFF3014 at 738.775 kb on + strand, within GFF3014 at 738.776 kb on - strand, within GFF3014 at 738.776 kb on - strand, within GFF3014 at 738.776 kb on - strand, within GFF3014 at 738.847 kb on + strand, within GFF3014 at 738.976 kb on + strand, within GFF3014 at 739.066 kb on + strand, within GFF3014 at 739.243 kb on + strand, within GFF3014 at 739.243 kb on + strand, within GFF3014 at 739.243 kb on + strand, within GFF3014 at 739.244 kb on - strand, within GFF3014 at 739.244 kb on - strand, within GFF3014 at 739.364 kb on - strand at 739.515 kb on + strand at 739.552 kb on - strand, within GFF3015 at 739.569 kb on + strand, within GFF3015 at 739.569 kb on + strand, within GFF3015 at 739.569 kb on + strand, within GFF3015 at 740.157 kb on + strand, within GFF3015 at 740.158 kb on - strand, within GFF3015 at 740.158 kb on - strand, within GFF3015 at 740.344 kb on + strand at 740.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 737,544 - GFF3012 0.74 -0.1 737,733 + -0.2 737,733 + +0.3 737,734 - +0.0 737,734 - -0.3 737,841 + GFF3013 0.16 -0.1 738,207 + GFF3013 0.65 +0.1 738,207 + GFF3013 0.65 +0.1 738,208 - GFF3013 0.66 +0.4 738,767 - GFF3014 0.32 -0.1 738,775 + GFF3014 0.32 +0.7 738,775 + GFF3014 0.32 +0.4 738,775 + GFF3014 0.32 +0.2 738,776 - GFF3014 0.32 +0.6 738,776 - GFF3014 0.32 +0.4 738,776 - GFF3014 0.32 +0.5 738,847 + GFF3014 0.40 -0.8 738,976 + GFF3014 0.53 +0.4 739,066 + GFF3014 0.62 +0.4 739,243 + GFF3014 0.80 -0.5 739,243 + GFF3014 0.80 -2.5 739,243 + GFF3014 0.80 +0.3 739,244 - GFF3014 0.80 -0.2 739,244 - GFF3014 0.80 +0.0 739,364 - -1.3 739,515 + -0.5 739,552 - GFF3015 0.13 -0.9 739,569 + GFF3015 0.15 +0.5 739,569 + GFF3015 0.15 -0.7 739,569 + GFF3015 0.15 +1.6 740,157 + GFF3015 0.83 -0.4 740,158 - GFF3015 0.84 +0.1 740,158 - GFF3015 0.84 +0.3 740,344 + +0.6 740,345 - +0.3
Or see this region's nucleotide sequence