Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF256 and GFF257 are separated by 369 nucleotides GFF257 and GFF258 are separated by 248 nucleotides
GFF256 - Biotin synthase, at 269,390 to 270,361
GFF256
GFF257 - Phosphomethylpyrimidine synthase, at 270,731 to 272,605
GFF257
GFF258 - tRNA dimethylallyltransferase, at 272,854 to 273,759
GFF258
Position (kb) 270
271
272
273 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 269.829 kb on + strand, within GFF256 at 269.830 kb on - strand, within GFF256 at 269.830 kb on - strand, within GFF256 at 269.890 kb on - strand, within GFF256 at 270.168 kb on + strand, within GFF256 at 270.168 kb on + strand, within GFF256 at 270.168 kb on + strand, within GFF256 at 270.402 kb on + strand at 270.500 kb on + strand at 270.500 kb on + strand at 270.502 kb on + strand at 270.503 kb on - strand at 270.551 kb on - strand at 270.679 kb on + strand at 270.679 kb on + strand at 270.680 kb on - strand at 270.680 kb on - strand at 270.685 kb on + strand at 270.685 kb on + strand at 270.885 kb on - strand at 270.885 kb on - strand at 270.885 kb on - strand at 270.903 kb on - strand at 270.905 kb on - strand at 270.905 kb on - strand at 270.905 kb on - strand at 270.906 kb on - strand at 271.124 kb on + strand, within GFF257 at 271.125 kb on - strand, within GFF257 at 271.160 kb on + strand, within GFF257 at 271.160 kb on + strand, within GFF257 at 271.160 kb on + strand, within GFF257 at 271.160 kb on + strand, within GFF257 at 271.161 kb on - strand, within GFF257 at 271.161 kb on - strand, within GFF257 at 271.379 kb on - strand, within GFF257 at 271.550 kb on + strand, within GFF257 at 271.550 kb on + strand, within GFF257 at 271.551 kb on - strand, within GFF257 at 271.551 kb on - strand, within GFF257 at 271.551 kb on - strand, within GFF257 at 271.646 kb on + strand, within GFF257 at 271.938 kb on - strand, within GFF257 at 271.938 kb on - strand, within GFF257 at 272.079 kb on - strand, within GFF257 at 272.144 kb on + strand, within GFF257 at 272.144 kb on + strand, within GFF257 at 272.144 kb on + strand, within GFF257 at 272.144 kb on + strand, within GFF257 at 272.145 kb on - strand, within GFF257 at 272.145 kb on - strand, within GFF257 at 272.210 kb on + strand, within GFF257 at 272.211 kb on - strand, within GFF257 at 272.550 kb on + strand at 272.550 kb on + strand at 272.550 kb on + strand at 272.551 kb on - strand at 272.551 kb on - strand at 272.555 kb on + strand at 272.555 kb on + strand at 272.556 kb on - strand at 272.937 kb on - strand at 273.458 kb on + strand, within GFF258 at 273.458 kb on + strand, within GFF258 at 273.487 kb on + strand, within GFF258 at 273.554 kb on + strand, within GFF258 at 273.581 kb on + strand, within GFF258
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 269,829 + GFF256 0.45 +0.7 269,830 - GFF256 0.45 +0.2 269,830 - GFF256 0.45 -1.5 269,890 - GFF256 0.51 +0.3 270,168 + GFF256 0.80 -0.1 270,168 + GFF256 0.80 -0.2 270,168 + GFF256 0.80 +0.3 270,402 + +0.0 270,500 + -0.6 270,500 + -2.2 270,502 + -0.4 270,503 - -1.8 270,551 - -2.1 270,679 + -0.5 270,679 + -2.7 270,680 - -0.9 270,680 - -1.2 270,685 + -0.7 270,685 + -0.6 270,885 - -1.1 270,885 - -1.0 270,885 - -1.5 270,903 - -1.2 270,905 - -1.0 270,905 - -0.8 270,905 - -2.1 270,906 - -2.4 271,124 + GFF257 0.21 -3.5 271,125 - GFF257 0.21 -2.0 271,160 + GFF257 0.23 -0.8 271,160 + GFF257 0.23 -1.9 271,160 + GFF257 0.23 -1.0 271,160 + GFF257 0.23 -1.1 271,161 - GFF257 0.23 -0.9 271,161 - GFF257 0.23 -2.5 271,379 - GFF257 0.35 -1.4 271,550 + GFF257 0.44 -1.8 271,550 + GFF257 0.44 -1.4 271,551 - GFF257 0.44 -0.5 271,551 - GFF257 0.44 -2.4 271,551 - GFF257 0.44 -1.6 271,646 + GFF257 0.49 -2.1 271,938 - GFF257 0.64 -0.7 271,938 - GFF257 0.64 -0.8 272,079 - GFF257 0.72 -1.1 272,144 + GFF257 0.75 -2.1 272,144 + GFF257 0.75 -2.8 272,144 + GFF257 0.75 -2.1 272,144 + GFF257 0.75 -2.0 272,145 - GFF257 0.75 -0.9 272,145 - GFF257 0.75 -1.4 272,210 + GFF257 0.79 -1.4 272,211 - GFF257 0.79 -1.9 272,550 + -0.4 272,550 + +0.0 272,550 + -0.7 272,551 - -0.2 272,551 - +0.4 272,555 + +0.3 272,555 + +0.9 272,556 - +0.6 272,937 - +0.2 273,458 + GFF258 0.67 +2.1 273,458 + GFF258 0.67 +2.6 273,487 + GFF258 0.70 +2.8 273,554 + GFF258 0.77 +2.3 273,581 + GFF258 0.80 +2.8
Or see this region's nucleotide sequence