Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2441 and GFF2442 are separated by 275 nucleotides GFF2442 and GFF2443 are separated by 220 nucleotides
GFF2441 - Ribulose-phosphate 3-epimerase, at 97,246 to 97,938
GFF2441
GFF2442 - Fructose-bisphosphate aldolase, at 98,214 to 99,278
GFF2442
GFF2443 - Transketolase 2, at 99,499 to 101,562
GFF2443
Position (kb) 98
99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 97.964 kb on - strand at 98.178 kb on + strand at 98.178 kb on + strand at 98.178 kb on + strand at 98.178 kb on + strand at 98.178 kb on + strand at 98.178 kb on + strand at 98.179 kb on - strand at 98.180 kb on + strand at 98.180 kb on + strand at 98.181 kb on - strand at 98.181 kb on - strand at 98.181 kb on - strand at 98.181 kb on - strand at 98.248 kb on + strand at 98.249 kb on - strand at 98.249 kb on - strand at 98.512 kb on + strand, within GFF2442 at 98.513 kb on - strand, within GFF2442 at 98.513 kb on - strand, within GFF2442 at 98.536 kb on + strand, within GFF2442 at 98.536 kb on + strand, within GFF2442 at 98.536 kb on + strand, within GFF2442 at 98.537 kb on - strand, within GFF2442 at 98.537 kb on - strand, within GFF2442 at 98.537 kb on - strand, within GFF2442 at 98.630 kb on + strand, within GFF2442 at 98.630 kb on + strand, within GFF2442 at 98.631 kb on - strand, within GFF2442 at 98.677 kb on + strand, within GFF2442 at 98.920 kb on + strand, within GFF2442 at 98.920 kb on + strand, within GFF2442 at 98.920 kb on + strand, within GFF2442 at 98.920 kb on + strand, within GFF2442 at 98.921 kb on - strand, within GFF2442 at 98.921 kb on - strand, within GFF2442 at 98.921 kb on - strand, within GFF2442 at 98.921 kb on - strand, within GFF2442 at 98.926 kb on + strand, within GFF2442 at 98.926 kb on + strand, within GFF2442 at 98.926 kb on + strand, within GFF2442 at 99.112 kb on + strand, within GFF2442 at 99.113 kb on - strand, within GFF2442 at 99.113 kb on - strand, within GFF2442 at 99.278 kb on + strand at 99.278 kb on + strand at 99.278 kb on + strand at 99.278 kb on + strand at 99.279 kb on - strand at 99.279 kb on - strand at 99.279 kb on - strand at 99.279 kb on - strand at 99.356 kb on + strand at 99.356 kb on + strand at 99.357 kb on - strand at 99.506 kb on - strand at 99.543 kb on + strand at 99.543 kb on + strand at 99.878 kb on + strand, within GFF2443 at 99.878 kb on + strand, within GFF2443 at 99.878 kb on + strand, within GFF2443 at 99.878 kb on + strand, within GFF2443 at 99.879 kb on - strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.130 kb on + strand, within GFF2443 at 100.131 kb on - strand, within GFF2443 at 100.131 kb on - strand, within GFF2443 at 100.131 kb on - strand, within GFF2443 at 100.131 kb on - strand, within GFF2443 at 100.131 kb on - strand, within GFF2443 at 100.181 kb on + strand, within GFF2443 at 100.182 kb on - strand, within GFF2443
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 97,964 - +0.6 98,178 + +0.3 98,178 + +1.3 98,178 + -0.1 98,178 + -0.9 98,178 + -0.1 98,178 + +0.2 98,179 - +0.1 98,180 + +0.7 98,180 + -0.4 98,181 - +0.2 98,181 - +0.4 98,181 - -0.3 98,181 - +0.4 98,248 + -0.9 98,249 - -0.7 98,249 - -2.3 98,512 + GFF2442 0.28 -0.8 98,513 - GFF2442 0.28 -1.2 98,513 - GFF2442 0.28 -1.6 98,536 + GFF2442 0.30 -1.9 98,536 + GFF2442 0.30 -0.5 98,536 + GFF2442 0.30 -0.6 98,537 - GFF2442 0.30 -1.3 98,537 - GFF2442 0.30 -1.3 98,537 - GFF2442 0.30 -0.7 98,630 + GFF2442 0.39 -0.7 98,630 + GFF2442 0.39 -0.4 98,631 - GFF2442 0.39 -1.3 98,677 + GFF2442 0.43 -0.3 98,920 + GFF2442 0.66 -0.9 98,920 + GFF2442 0.66 -1.6 98,920 + GFF2442 0.66 -0.1 98,920 + GFF2442 0.66 -0.4 98,921 - GFF2442 0.66 -1.9 98,921 - GFF2442 0.66 -1.2 98,921 - GFF2442 0.66 +0.1 98,921 - GFF2442 0.66 -2.0 98,926 + GFF2442 0.67 -0.7 98,926 + GFF2442 0.67 -1.0 98,926 + GFF2442 0.67 -2.2 99,112 + GFF2442 0.84 -1.8 99,113 - GFF2442 0.84 -0.8 99,113 - GFF2442 0.84 -3.3 99,278 + -0.3 99,278 + -0.6 99,278 + -1.7 99,278 + -1.4 99,279 - -0.9 99,279 - -0.6 99,279 - -1.3 99,279 - -1.4 99,356 + -0.4 99,356 + -0.4 99,357 - -0.0 99,506 - -0.5 99,543 + -1.0 99,543 + +0.4 99,878 + GFF2443 0.18 +0.3 99,878 + GFF2443 0.18 -0.0 99,878 + GFF2443 0.18 +0.1 99,878 + GFF2443 0.18 -0.5 99,879 - GFF2443 0.18 -0.4 100,130 + GFF2443 0.31 -0.9 100,130 + GFF2443 0.31 -0.4 100,130 + GFF2443 0.31 +0.5 100,130 + GFF2443 0.31 +0.4 100,130 + GFF2443 0.31 +0.8 100,130 + GFF2443 0.31 -0.2 100,130 + GFF2443 0.31 -0.6 100,130 + GFF2443 0.31 +0.2 100,131 - GFF2443 0.31 +1.1 100,131 - GFF2443 0.31 -0.7 100,131 - GFF2443 0.31 -0.1 100,131 - GFF2443 0.31 +0.7 100,131 - GFF2443 0.31 -0.3 100,181 + GFF2443 0.33 -0.8 100,182 - GFF2443 0.33 +0.6
Or see this region's nucleotide sequence