Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2399 and GFF2400 are separated by 341 nucleotides GFF2400 and GFF2401 are separated by 85 nucleotides
GFF2399 - hypothetical protein, at 50,622 to 51,620
GFF2399
GFF2400 - 2-isopropylmalate synthase, at 51,962 to 53,524
GFF2400
GFF2401 - hypothetical protein, at 53,610 to 55,652
GFF2401
Position (kb) 51
52
53
54 Strain fitness (log2 ratio) -2
-1
0
1
2 at 50.976 kb on + strand, within GFF2399 at 50.977 kb on - strand, within GFF2399 at 50.977 kb on - strand, within GFF2399 at 50.977 kb on - strand, within GFF2399 at 51.119 kb on - strand, within GFF2399 at 51.249 kb on + strand, within GFF2399 at 51.279 kb on + strand, within GFF2399 at 51.279 kb on + strand, within GFF2399 at 51.279 kb on + strand, within GFF2399 at 51.280 kb on - strand, within GFF2399 at 51.280 kb on - strand, within GFF2399 at 51.280 kb on - strand, within GFF2399 at 51.280 kb on - strand, within GFF2399 at 51.544 kb on - strand at 51.544 kb on - strand at 51.573 kb on + strand at 51.573 kb on + strand at 51.574 kb on - strand at 51.574 kb on - strand at 51.574 kb on - strand at 51.644 kb on + strand at 51.645 kb on - strand at 51.645 kb on - strand at 51.788 kb on + strand at 51.789 kb on - strand at 51.903 kb on + strand at 52.310 kb on + strand, within GFF2400 at 52.310 kb on + strand, within GFF2400 at 52.310 kb on + strand, within GFF2400 at 52.310 kb on + strand, within GFF2400 at 52.311 kb on - strand, within GFF2400 at 52.311 kb on - strand, within GFF2400 at 52.311 kb on - strand, within GFF2400 at 52.343 kb on + strand, within GFF2400 at 52.523 kb on + strand, within GFF2400 at 52.523 kb on + strand, within GFF2400 at 52.746 kb on + strand, within GFF2400 at 52.746 kb on + strand, within GFF2400 at 52.747 kb on - strand, within GFF2400 at 52.833 kb on - strand, within GFF2400 at 52.833 kb on - strand, within GFF2400 at 52.906 kb on + strand, within GFF2400 at 52.916 kb on + strand, within GFF2400 at 53.022 kb on - strand, within GFF2400 at 53.328 kb on - strand, within GFF2400 at 53.431 kb on + strand at 53.456 kb on + strand at 53.456 kb on + strand at 53.564 kb on + strand at 53.564 kb on + strand at 54.104 kb on + strand, within GFF2401
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 50,976 + GFF2399 0.35 -0.0 50,977 - GFF2399 0.36 -1.0 50,977 - GFF2399 0.36 +0.6 50,977 - GFF2399 0.36 +1.1 51,119 - GFF2399 0.50 +0.4 51,249 + GFF2399 0.63 +1.3 51,279 + GFF2399 0.66 +0.7 51,279 + GFF2399 0.66 +0.2 51,279 + GFF2399 0.66 +1.4 51,280 - GFF2399 0.66 -0.1 51,280 - GFF2399 0.66 -0.3 51,280 - GFF2399 0.66 -1.0 51,280 - GFF2399 0.66 +1.0 51,544 - +1.5 51,544 - +0.6 51,573 + +0.8 51,573 + +2.2 51,574 - +0.7 51,574 - +0.3 51,574 - +1.1 51,644 + +1.2 51,645 - +0.1 51,645 - -0.7 51,788 + +1.6 51,789 - +1.4 51,903 + +1.3 52,310 + GFF2400 0.22 +0.1 52,310 + GFF2400 0.22 -0.4 52,310 + GFF2400 0.22 -0.5 52,310 + GFF2400 0.22 -2.4 52,311 - GFF2400 0.22 -1.8 52,311 - GFF2400 0.22 -0.9 52,311 - GFF2400 0.22 -1.3 52,343 + GFF2400 0.24 +0.1 52,523 + GFF2400 0.36 -0.3 52,523 + GFF2400 0.36 -1.0 52,746 + GFF2400 0.50 +0.3 52,746 + GFF2400 0.50 -0.4 52,747 - GFF2400 0.50 -1.1 52,833 - GFF2400 0.56 +1.1 52,833 - GFF2400 0.56 -0.7 52,906 + GFF2400 0.60 -0.6 52,916 + GFF2400 0.61 -1.4 53,022 - GFF2400 0.68 -1.1 53,328 - GFF2400 0.87 +1.3 53,431 + +1.5 53,456 + +2.3 53,456 + +1.8 53,564 + +1.1 53,564 + +1.7 54,104 + GFF2401 0.24 +0.6
Or see this region's nucleotide sequence