Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2220 and GFF2221 are separated by 110 nucleotides GFF2221 and GFF2222 are separated by 20 nucleotides GFF2222 and GFF2223 are separated by 52 nucleotides GFF2223 and GFF2224 are separated by 17 nucleotides
GFF2220 - Intracellular septation protein, at 926,336 to 926,935
GFF2220
GFF2221 - Biosynthetic peptidoglycan transglycosylase, at 927,046 to 927,717
GFF2221
GFF2222 - Farnesyl diphosphate synthase, at 927,738 to 928,772
GFF2222
GFF2223 - 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase, at 928,825 to 929,448
GFF2223
GFF2224 - 4-hydroxy-tetrahydrodipicolinate reductase, at 929,466 to 930,293
GFF2224
Position (kb) 927
928
929 Strain fitness (log2 ratio) -1
0
1 at 926.812 kb on - strand, within GFF2220 at 926.812 kb on - strand, within GFF2220 at 927.296 kb on + strand, within GFF2221 at 927.297 kb on - strand, within GFF2221 at 927.374 kb on + strand, within GFF2221 at 927.374 kb on + strand, within GFF2221 at 927.375 kb on - strand, within GFF2221 at 927.650 kb on + strand at 927.651 kb on - strand at 927.685 kb on + strand at 927.685 kb on + strand at 927.685 kb on + strand at 927.686 kb on - strand at 927.835 kb on + strand at 928.101 kb on + strand, within GFF2222 at 928.101 kb on + strand, within GFF2222 at 928.102 kb on - strand, within GFF2222 at 928.102 kb on - strand, within GFF2222 at 928.179 kb on + strand, within GFF2222 at 928.179 kb on + strand, within GFF2222
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 926,812 - GFF2220 0.79 -1.0 926,812 - GFF2220 0.79 -0.3 927,296 + GFF2221 0.37 +0.7 927,297 - GFF2221 0.37 +0.7 927,374 + GFF2221 0.49 -0.7 927,374 + GFF2221 0.49 -1.3 927,375 - GFF2221 0.49 -0.1 927,650 + -0.2 927,651 - -0.5 927,685 + -0.2 927,685 + +0.4 927,685 + -0.1 927,686 - -0.1 927,835 + +0.1 928,101 + GFF2222 0.35 -0.1 928,101 + GFF2222 0.35 -1.1 928,102 - GFF2222 0.35 -1.7 928,102 - GFF2222 0.35 -0.1 928,179 + GFF2222 0.43 +0.3 928,179 + GFF2222 0.43 -0.7
Or see this region's nucleotide sequence