Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2064 and GFF2065 are separated by 275 nucleotides GFF2065 and GFF2066 are separated by 15 nucleotides GFF2066 and GFF2067 overlap by 4 nucleotides
GFF2064 - Aldehyde dehydrogenase, at 742,877 to 744,397
GFF2064
GFF2065 - Acetoin catabolism regulatory protein, at 744,673 to 746,610
GFF2065
GFF2066 - 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB, at 746,626 to 747,432
GFF2066
GFF2067 - HTH-type transcriptional regulator DegA, at 747,429 to 748,526
GFF2067
Position (kb) 744
745
746
747 Strain fitness (log2 ratio) -2
-1
0
1
2 at 743.946 kb on + strand, within GFF2064 at 743.947 kb on - strand, within GFF2064 at 743.952 kb on + strand, within GFF2064 at 744.399 kb on - strand at 744.471 kb on + strand at 744.560 kb on - strand at 744.634 kb on + strand at 744.634 kb on + strand at 744.634 kb on + strand at 744.634 kb on + strand at 745.151 kb on - strand, within GFF2065 at 745.151 kb on - strand, within GFF2065 at 746.123 kb on - strand, within GFF2065 at 746.140 kb on + strand, within GFF2065 at 746.140 kb on + strand, within GFF2065 at 746.140 kb on + strand, within GFF2065 at 746.141 kb on - strand, within GFF2065 at 746.141 kb on - strand, within GFF2065 at 746.141 kb on - strand, within GFF2065 at 746.278 kb on - strand, within GFF2065 at 746.627 kb on + strand at 746.628 kb on - strand at 746.628 kb on - strand at 747.024 kb on + strand, within GFF2066 at 747.024 kb on + strand, within GFF2066 at 747.024 kb on + strand, within GFF2066 at 747.024 kb on + strand, within GFF2066 at 747.024 kb on + strand, within GFF2066 at 747.418 kb on + strand at 747.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 743,946 + GFF2064 0.70 -2.3 743,947 - GFF2064 0.70 -0.2 743,952 + GFF2064 0.71 +2.1 744,399 - +0.1 744,471 + -1.2 744,560 - +0.2 744,634 + +0.6 744,634 + +1.3 744,634 + +0.1 744,634 + +0.4 745,151 - GFF2065 0.25 -0.2 745,151 - GFF2065 0.25 -0.6 746,123 - GFF2065 0.75 +1.1 746,140 + GFF2065 0.76 -0.3 746,140 + GFF2065 0.76 -0.0 746,140 + GFF2065 0.76 -0.5 746,141 - GFF2065 0.76 -0.9 746,141 - GFF2065 0.76 -0.3 746,141 - GFF2065 0.76 +0.3 746,278 - GFF2065 0.83 +0.3 746,627 + +0.1 746,628 - +0.5 746,628 - -0.1 747,024 + GFF2066 0.49 +0.1 747,024 + GFF2066 0.49 +0.2 747,024 + GFF2066 0.49 +0.2 747,024 + GFF2066 0.49 -0.3 747,024 + GFF2066 0.49 -0.6 747,418 + +0.1 747,433 - +0.4
Or see this region's nucleotide sequence