Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2043 and GFF2044 are separated by 37 nucleotides GFF2044 and GFF2045 are separated by 16 nucleotides GFF2045 and GFF2046 are separated by 142 nucleotides GFF2046 and GFF2047 are separated by 75 nucleotides GFF2047 and GFF2048 are separated by 85 nucleotides
GFF2043 - Peptide deformylase, at 716,847 to 717,365
GFF2043
GFF2044 - Methionyl-tRNA formyltransferase, at 717,403 to 718,326
GFF2044
GFF2045 - tRNA pseudouridine synthase A, at 718,343 to 719,080
GFF2045
GFF2046 - hypothetical protein, at 719,223 to 719,573
GFF2046
GFF2047 - Thioredoxin C-1, at 719,649 to 719,969
GFF2047
GFF2048 - RecBCD enzyme subunit RecB, at 720,055 to 723,573
GFF2048
Position (kb)
718
719
720 Strain fitness (log2 ratio)
-1
0
1 at 718.325 kb on - strand at 718.325 kb on - strand at 718.371 kb on - strand at 718.673 kb on + strand, within GFF2045 at 718.674 kb on - strand, within GFF2045 at 718.869 kb on + strand, within GFF2045 at 718.870 kb on - strand, within GFF2045 at 719.057 kb on + strand at 719.228 kb on + strand at 719.237 kb on + strand at 719.982 kb on - strand at 720.023 kb on - strand at 720.023 kb on - strand at 720.023 kb on - strand at 720.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 718,325 - -0.9 718,325 - +0.1 718,371 - -1.6 718,673 + GFF2045 0.45 -0.9 718,674 - GFF2045 0.45 +0.5 718,869 + GFF2045 0.71 -1.5 718,870 - GFF2045 0.71 -0.2 719,057 + -1.7 719,228 + -0.9 719,237 + -0.5 719,982 - +0.2 720,023 - -0.4 720,023 - -0.0 720,023 - -0.4 720,023 - +0.0
Or see this region's nucleotide sequence