Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF203 and GFF204 are separated by 68 nucleotides GFF204 and GFF205 are separated by 95 nucleotides GFF205 and GFF206 are separated by 82 nucleotides
GFF203 - Serine acetyltransferase, at 214,084 to 214,968
GFF203
GFF204 - hypothetical protein, at 215,037 to 215,423
GFF204
GFF205 - 3-oxoadipate enol-lactonase 2, at 215,519 to 216,292
GFF205
GFF206 - Cadmium, cobalt and zinc/H(+)-K(+) antiporter, at 216,375 to 217,358
GFF206
Position (kb) 215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 214.047 kb on + strand at 214.047 kb on + strand at 214.047 kb on + strand at 214.047 kb on + strand at 215.005 kb on - strand at 215.005 kb on - strand at 215.005 kb on - strand at 215.031 kb on + strand at 215.071 kb on - strand at 215.072 kb on + strand at 215.072 kb on + strand at 215.206 kb on - strand, within GFF204 at 215.247 kb on + strand, within GFF204 at 215.323 kb on - strand, within GFF204 at 215.481 kb on + strand at 215.718 kb on - strand, within GFF205 at 215.718 kb on - strand, within GFF205 at 215.718 kb on - strand, within GFF205 at 215.718 kb on - strand, within GFF205 at 215.718 kb on - strand, within GFF205 at 216.056 kb on + strand, within GFF205 at 216.057 kb on - strand, within GFF205 at 216.057 kb on - strand, within GFF205
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 214,047 + -0.1 214,047 + +1.2 214,047 + +0.0 214,047 + -0.1 215,005 - +0.3 215,005 - -2.1 215,005 - -0.2 215,031 + -2.4 215,071 - -0.7 215,072 + +0.6 215,072 + -1.0 215,206 - GFF204 0.44 -1.5 215,247 + GFF204 0.54 -0.3 215,323 - GFF204 0.74 -2.6 215,481 + +0.6 215,718 - GFF205 0.26 -0.2 215,718 - GFF205 0.26 -0.6 215,718 - GFF205 0.26 -0.0 215,718 - GFF205 0.26 -0.5 215,718 - GFF205 0.26 +0.4 216,056 + GFF205 0.69 +0.6 216,057 - GFF205 0.70 +0.4 216,057 - GFF205 0.70 +0.2
Or see this region's nucleotide sequence