Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1953 and GFF1954 are separated by 194 nucleotides GFF1954 and GFF1955 are separated by 232 nucleotides GFF1955 and GFF1956 are separated by 102 nucleotides GFF1956 and GFF1957 are separated by 47 nucleotides
GFF1953 - Nicotinate phosphoribosyltransferase, at 608,358 to 609,650
GFF1953
GFF1954 - hypothetical protein, at 609,845 to 610,132
GFF1954
GFF1955 - Glyoxylate carboligase, at 610,365 to 612,140
GFF1955
GFF1956 - Hydroxypyruvate isomerase, at 612,243 to 613,022
GFF1956
GFF1957 - 2-hydroxy-3-oxopropionate reductase, at 613,070 to 613,957
GFF1957
Position (kb) 610
611
612
613 Strain fitness (log2 ratio) -1
0
1 at 609.373 kb on + strand, within GFF1953 at 609.373 kb on + strand, within GFF1953 at 609.373 kb on + strand, within GFF1953 at 609.374 kb on - strand, within GFF1953 at 609.568 kb on + strand at 609.732 kb on + strand at 609.736 kb on + strand at 609.736 kb on + strand at 609.737 kb on - strand at 609.737 kb on - strand at 609.737 kb on - strand at 609.738 kb on + strand at 609.979 kb on - strand, within GFF1954 at 609.979 kb on - strand, within GFF1954 at 609.993 kb on + strand, within GFF1954 at 610.240 kb on + strand at 610.264 kb on - strand at 610.313 kb on + strand at 610.314 kb on - strand at 610.314 kb on - strand at 610.342 kb on + strand at 610.342 kb on + strand at 610.342 kb on + strand at 610.342 kb on + strand at 610.342 kb on + strand at 610.343 kb on - strand at 610.343 kb on - strand at 610.343 kb on - strand at 610.464 kb on + strand at 610.465 kb on - strand at 610.545 kb on + strand, within GFF1955 at 610.546 kb on - strand, within GFF1955 at 610.568 kb on + strand, within GFF1955 at 610.626 kb on + strand, within GFF1955 at 610.627 kb on - strand, within GFF1955 at 610.692 kb on + strand, within GFF1955 at 610.692 kb on + strand, within GFF1955 at 610.693 kb on - strand, within GFF1955 at 610.896 kb on + strand, within GFF1955 at 610.896 kb on + strand, within GFF1955 at 610.896 kb on + strand, within GFF1955 at 610.897 kb on - strand, within GFF1955 at 610.897 kb on - strand, within GFF1955 at 610.897 kb on - strand, within GFF1955 at 610.897 kb on - strand, within GFF1955 at 610.915 kb on - strand, within GFF1955 at 611.238 kb on + strand, within GFF1955 at 611.238 kb on + strand, within GFF1955 at 611.490 kb on + strand, within GFF1955 at 611.490 kb on + strand, within GFF1955 at 611.491 kb on - strand, within GFF1955 at 611.491 kb on - strand, within GFF1955 at 611.529 kb on + strand, within GFF1955 at 611.530 kb on - strand, within GFF1955 at 611.530 kb on - strand, within GFF1955 at 611.530 kb on - strand, within GFF1955 at 611.530 kb on - strand, within GFF1955 at 611.584 kb on - strand, within GFF1955 at 611.584 kb on - strand, within GFF1955 at 611.584 kb on - strand, within GFF1955 at 611.584 kb on - strand, within GFF1955 at 611.584 kb on - strand, within GFF1955 at 611.704 kb on - strand, within GFF1955 at 611.704 kb on - strand, within GFF1955 at 611.760 kb on + strand, within GFF1955 at 611.788 kb on - strand, within GFF1955 at 611.788 kb on - strand, within GFF1955 at 612.138 kb on + strand at 612.138 kb on + strand at 612.161 kb on + strand at 612.161 kb on + strand at 612.343 kb on - strand, within GFF1956 at 612.396 kb on + strand, within GFF1956 at 612.396 kb on + strand, within GFF1956 at 612.396 kb on + strand, within GFF1956 at 612.397 kb on - strand, within GFF1956 at 612.397 kb on - strand, within GFF1956 at 612.397 kb on - strand, within GFF1956 at 612.397 kb on - strand, within GFF1956 at 612.519 kb on + strand, within GFF1956 at 612.592 kb on - strand, within GFF1956 at 612.780 kb on + strand, within GFF1956 at 612.780 kb on + strand, within GFF1956 at 612.781 kb on - strand, within GFF1956 at 612.940 kb on - strand, within GFF1956 at 612.964 kb on - strand at 612.964 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 609,373 + GFF1953 0.78 +0.6 609,373 + GFF1953 0.78 -1.0 609,373 + GFF1953 0.78 +0.1 609,374 - GFF1953 0.79 -0.3 609,568 + -0.9 609,732 + -0.5 609,736 + +0.7 609,736 + -0.1 609,737 - -0.5 609,737 - +0.0 609,737 - +0.5 609,738 + -1.6 609,979 - GFF1954 0.47 -0.1 609,979 - GFF1954 0.47 -1.1 609,993 + GFF1954 0.51 -0.1 610,240 + +1.5 610,264 - +1.1 610,313 + -0.6 610,314 - -0.2 610,314 - +0.2 610,342 + +0.1 610,342 + -0.1 610,342 + +0.3 610,342 + -0.2 610,342 + +0.7 610,343 - +1.7 610,343 - +0.4 610,343 - +0.3 610,464 + +1.3 610,465 - +0.0 610,545 + GFF1955 0.10 +0.2 610,546 - GFF1955 0.10 +0.6 610,568 + GFF1955 0.11 +1.3 610,626 + GFF1955 0.15 -0.3 610,627 - GFF1955 0.15 +0.5 610,692 + GFF1955 0.18 +0.7 610,692 + GFF1955 0.18 -1.0 610,693 - GFF1955 0.18 -0.2 610,896 + GFF1955 0.30 -0.4 610,896 + GFF1955 0.30 +0.1 610,896 + GFF1955 0.30 -0.0 610,897 - GFF1955 0.30 -0.7 610,897 - GFF1955 0.30 +0.0 610,897 - GFF1955 0.30 -0.3 610,897 - GFF1955 0.30 +0.0 610,915 - GFF1955 0.31 +0.1 611,238 + GFF1955 0.49 +0.5 611,238 + GFF1955 0.49 +0.4 611,490 + GFF1955 0.63 +0.0 611,490 + GFF1955 0.63 +0.4 611,491 - GFF1955 0.63 +0.7 611,491 - GFF1955 0.63 -0.3 611,529 + GFF1955 0.66 +0.3 611,530 - GFF1955 0.66 +0.4 611,530 - GFF1955 0.66 +0.3 611,530 - GFF1955 0.66 -0.4 611,530 - GFF1955 0.66 +0.2 611,584 - GFF1955 0.69 +0.4 611,584 - GFF1955 0.69 -1.0 611,584 - GFF1955 0.69 -0.4 611,584 - GFF1955 0.69 +0.8 611,584 - GFF1955 0.69 +0.3 611,704 - GFF1955 0.75 -0.4 611,704 - GFF1955 0.75 +0.8 611,760 + GFF1955 0.79 -1.0 611,788 - GFF1955 0.80 -0.0 611,788 - GFF1955 0.80 +0.0 612,138 + -0.6 612,138 + -0.2 612,161 + -0.8 612,161 + -0.5 612,343 - GFF1956 0.13 +0.1 612,396 + GFF1956 0.20 +0.2 612,396 + GFF1956 0.20 +0.8 612,396 + GFF1956 0.20 -0.0 612,397 - GFF1956 0.20 +0.3 612,397 - GFF1956 0.20 +0.1 612,397 - GFF1956 0.20 -0.2 612,397 - GFF1956 0.20 +0.7 612,519 + GFF1956 0.35 +0.4 612,592 - GFF1956 0.45 +0.5 612,780 + GFF1956 0.69 +0.5 612,780 + GFF1956 0.69 -0.9 612,781 - GFF1956 0.69 -0.4 612,940 - GFF1956 0.89 -0.5 612,964 - +0.0 612,964 - +0.9
Or see this region's nucleotide sequence