Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1894 and GFF1895 are separated by 121 nucleotides GFF1895 and GFF1896 are separated by 18 nucleotides GFF1896 and GFF1897 are separated by 147 nucleotides GFF1897 and GFF1898 are separated by 191 nucleotides
GFF1894 - Heat-inducible transcription repressor HrcA, at 553,846 to 554,946
GFF1894
GFF1895 - Protein GrpE, at 555,068 to 555,715
GFF1895
GFF1896 - hypothetical protein, at 555,734 to 556,168
GFF1896
GFF1897 - Elongation factor P, at 556,316 to 556,885
GFF1897
GFF1898 - Elongation factor P--(R)-beta-lysine ligase, at 557,077 to 558,150
GFF1898
Position (kb) 555
556
557 Strain fitness (log2 ratio) -1
0
1 at 554.864 kb on - strand at 554.864 kb on - strand at 554.951 kb on + strand at 555.735 kb on + strand at 555.735 kb on + strand at 555.735 kb on + strand at 555.736 kb on - strand at 555.739 kb on + strand at 555.853 kb on + strand, within GFF1896 at 555.855 kb on + strand, within GFF1896 at 555.856 kb on - strand, within GFF1896 at 555.916 kb on - strand, within GFF1896 at 556.081 kb on - strand, within GFF1896 at 556.125 kb on + strand at 556.126 kb on - strand at 556.174 kb on + strand at 556.175 kb on - strand at 556.175 kb on - strand at 556.175 kb on - strand at 557.072 kb on - strand at 557.072 kb on - strand at 557.072 kb on - strand at 557.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 554,864 - -0.6 554,864 - -0.7 554,951 + -0.2 555,735 + +0.5 555,735 + -0.2 555,735 + -0.6 555,736 - +0.1 555,739 + -0.0 555,853 + GFF1896 0.27 -1.6 555,855 + GFF1896 0.28 +0.7 555,856 - GFF1896 0.28 -0.5 555,916 - GFF1896 0.42 -0.8 556,081 - GFF1896 0.80 -1.3 556,125 + -1.4 556,126 - -0.1 556,174 + -0.0 556,175 - -0.7 556,175 - +0.4 556,175 - -1.1 557,072 - -0.9 557,072 - +1.5 557,072 - -0.4 557,072 - -0.7
Or see this region's nucleotide sequence